A biodiversity evaluation framework for restoration of aquatic macrophyte communities in shallow lakes driven by hydrological process management

Macrophyte community diversity and composition respond to ecosystem conservation and local environmental factors. In this study, we developed a multidimensional diversity framework for macrophyte communities, including the taxonomic and functional alpha and beta diversity. We used the framework to explore the relationships among water level regimes and these diversity parameters in a case study of China's Baiyangdian Lake. Analysis of indicators of hydrologic alteration divided the water level from 1959 to 2019 into four regimes (dry, <6.42 m; low, 6.42–7.23 m; medium, 7.23–8.19 m; high, >8.19 m). Alpha and beta diversity were significantly higher in the medium regime than in the low and high regimes. Redundancy analysis indicated that the maximum water depth significantly affected taxonomic alpha diversity, and total nitrogen (TN) and chemical oxygen demand (COD) concentration significantly affected functional alpha diversity, respectively. Mantel tests showed that TN, Secchi depth (SD), and water depth in the high water level regime significantly increased the total beta diversity and turnover components. TN was the main factor that increased total taxonomic beta diversity. Water level regime mainly influenced interspecific relationships by changing the TN and COD concentration. The water level should be maintained between the medium and high water level regimes to promote restoration of the macrophyte community and improve ecosystem stability. The biodiversity evaluation framework would provide a deeper insight into the hydrological process management for restoration of aquatic macrophyte communities in shallow lakes.     

Upwelling and river runoff as sources of dissolved nitrous oxide to the Alsea estuary,Oregon

Surface waters of Alsea Bay, an unpolluted estuary on the Oregon coast, were analysed for nitrous oxide, nitrate and nitrite on a weekly or biweekly basis during the summer of 1979. The estuary was found to be a variable source of N₂O to the atmosphere. Large and rapid increases in the concentrations of N₂O, NO₃^?, and NO₂^? occurred at the beginning of the sampling period and are attributed to the influx of nutrient-rich upwelling water into the estuary with the tide. The subsequent decline in concentrations of nitrate, nitrite and nitrous oxide over the remainder of the summer is attributed to a decrease in upwelling intensity, a decline in nitrification rates and to assimilatory nitrate reduction. Measurements of nitrous oxide at six stations along the Alsea River were also made in September and October before and after the onset of the rainy season. Samples taken after flood conditions were established were systematically 50% higher than pre-flood samples. The data suggest that soil runoff results in elevated concentrations of N₂O in rivers.     

A taxonomical and ecological survey of rotiffer communities in Krottensee (Austria)

The composition and distribution of benthic, periphytic and planktonic rotifers in different habitats in Krottensee were investigated. Sixty-five rotifer taxa were identified, their relative abundance estimated. Classification of the data set by cluster analysis allowed the recognition of distinct habitat groups confined to acid bog ponds, macrophytes, sediments and open water. Five groups of rotifer taxa were identified on the basis of their habitat preferences and the occurrence of the taxa. Highest diversities were found on macrophytes and in acid bog ponds.     

Distribution and Diversity of Ibla cumingi Darwin [Crustacea, Cirripedia) from the Gulf of Elat (Red Sea)

Abstract. The distribution of Ibla cumingi DARWIN on different types of hard substrata along 30 km of coast in the Gulf of Elat was studied. I. cumingi was found among and underneath Tetraclita squamosa rufotincta , in oyster beds, underneath slabs of beachrock, and in fissures in igneous rocks. In the Tetraclita belt, I. cumingi is most frequent in empty shells of dead Tetraclita while in the oyster bed it is common inside the meshwork made by the oysters. The distribution is regarded as clustered distribution. The density and size of the clusters of I. cumingi is determined by the available sheltered space within the midlittoral zone. The form of the habitat determines the shape of the animals. Those found in the oyster bed and fissures are usually longer than those found in the Tetraclita belt due to the depth of the fissures or to the bigger spaces within the oyster bed. I. cumingi is orientated so that the capitulum with the cirri is projecting towards the open water, and the water current. It is concluded that I. cumingi can withstand the physical stress encountered in a tropical intertidal zone, but that the distribution pattern is caused by predation.     

Application of Microbial BOD Sensors in Marine Monitoring

A strain of yeast, which can endure high osmotic pressure, is employed for the sensitive material of the microbial BOD sensor. Two immobilization methods are used, I.e. Calcium alginate gel be ads and PV A gel beads. The results show that the PVA gel beads is better. The influences of osmosis and heavy metal ions on the yeast entrapped in the PVA gel beads are also studied in the experiment.     

海桑属红树植物遗传多样性和引种关系研究

以海南东寨港红树林自然保护区内无瓣海桑(Sonneratia apetala)、海南海桑(S.hainanensis)、拟海桑(S.paracaseolaris)、杯萼海桑(S.abla)、大叶海桑(S.ovata)、海桑(S.caseolaris)等6种海桑属红树植物为材料,对15个有效引物进行RAPD分析,共扩增出512条带,其中多态性条带为297,占总扩增条带的58.01%.Nei指数法分析和UPGMA统计分析表明,6种海桑属红树植物分为A,B,C3个组,平均遗传距离为0.38.A组包括无瓣海桑、海南海桑、大叶海桑、怀萼海桑,其中无瓣海桑、海南海桑、大叶海桑处于同一个亚组.B组包括拟海桑.C组包括海桑.对海南和福建无瓣海桑种群进行RAPD分析.对Shannon表型多样性指数统计结果表明,福建种群为0.669,海南种群为0.671,各种群遗传变异较大,这与无瓣海桑种群广泛的适应性相一致.对种群间的Shannon表型多样性指数分析表明,种群内的遗传变异占整个遗传变异的93.3%,而种群间的遗传变异仅占6.7%.这表明无瓣海桑种群的大部分遗传变异存在于种群内,而种群间的遗传变异较小.由此可见,无瓣海桑基因组丰富的多样性,是使其由海南成功引种到福建的重要因素.     

翘嘴红鲌(Erythroculter ilishaeformis Bleeker)种群遗传多样性的RAPD分析

采用RAPD技术对翘嘴红鲌养殖群体进行了DNA多态性检测.共采用40个随机引物进行扩增,其中14个引物可获得清晰且重复性好的扩增图谱,共扩增出121个RAPD位点,具有多态现象的位点为40个,多态位点比例为33.06%.翘嘴红鲌群体的遗传相似系数为 0.8878-0.9600,平均为0.9201,遗传变异度为0.0799.Shannon多样性指数为10.8824,Shannon多样性值为0.0899.研究表明,翘嘴红鲌目前的种质资源状况令人堪忧,改变目前人工繁育模式,建立原种、良种场,丰富物种遗传多样性是资源保护的基础.     

Study on genetic diversity of cultivated populations in 4 species of shrimps

Genetic diversity of cultivated populations was investigated using assay of vertical slabpolyacrylamide gel electrophoresis in 4 species of shrimps, which were Penaeus japonicus, Penaeus monodon , Penaeus vannamei and Metapenaeus ensis, The results showed that the mean number of alle-les per locus (A) is 1.3±0.2, 1.3±0.1, 1.3±0.1 and 1.3 ±0.1 respectively; the percentage of polymorphic loci (P0.95) is 12.5, 6.7, 20 and 23.5 respectively; the expected heterozygosity (He) is 0.042±0.034,0.042±0.031, 0.094±0.042 and 0.097±0.047 respectively; and the observed heterozygosity (H0)is 0.029±0.024, 0.028±0.023,0.154±0.082 and 0.150±0.084 respectively. The difference of genetic diversity is obvious in 4 species of shrimps. The degree of genetic diversity is M. ensis > P. vannamei > P. japonicus > P. monodon. In short, the lower level of genetic diversity is estimated in 4 species of shrimps.