Minor planet short periodic perturbations: The indirect part of the disturbing function

Leverrier's development of the indirect part of the disturbing function has been extended to include terms up to degree 4 in eccentricity and inclination; the resulting series has been expressed with respect to a fixed plane, and in a computer readable form (a list of integers). Tests have been performed for the relative significance of the terms of degrees 2, 3 and 4, and estimates have been obtained for the accuracy of the short periodic perturbations of a minor planet, and of the corresponding mean orbital elements. It was found that: (i) even in extreme cases, the indirect part of the disturbing function gives rise to very small short periodic perturbations; (ii) bodies of very high eccentricity/inclination and those close to mean motion resonances are most significantly affected; (iii) indirect perturbations for minor planets can be computed up to the degree 2 terms only, without any significant loss of accuracy; and (iv) higher degree indirect perturbations appear to be important only for their contribution to the long periodic effects of higher order (with respect to the perturbing mass).     

Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay

Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size, 0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m, which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell⁻¹ obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29) and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas this fraction was reduced in the middle layer.     

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.     

藓羽藻(Bryopsis hypnoides)核酮糖-1，5-二磷酸羧化酶／加氧酶的分离与活性测定

采用硫酸铵分部沉淀与凝胶过滤的方法，进行藓羽藻Rubisco的分离研究。结果表明，分离的藓羽藻Rubisco经SDS-聚丙烯酰胺凝胶电泳检测呈两条清晰条带，分别为Rubisco大亚基与小亚基；与菠菜相比，藓羽藻Rubisco大亚基分子量与菠菜基本相同，而小亚基较之稍大一些。藓羽藻Rubisco活力测定结果表明，Rubisco分离过程中用硫酸铵分部沉淀后活力降低许多，分离后活力有所上升，但仍比粗提液活力弱；在Rubisco活力测定过程中，藓羽藻Rubisco的活化温度与其它物种Rubisco活化的温度不同，在低温下活化效果较好。这些结果说明Rubisco的酶活力受硫酸铵的影响而且藓羽藻Rubisco相对陆地高等植物结构不稳定。     

遥感技术在地质找矿中的应用及发展前景

遥感技术作为矿产勘查的一种手段应用于找矿,并取得了一定成就.遥感技术的直接应用是蚀变遥感信息的提取,遥感技术的间接应用包括地质构造信息、植被的光谱特征及矿床改造信息等方面.遥感找矿具有很大的发展前景的领域主要有:高光谱数据、数据融合技术、3S的紧密结合、计算机技术的发展.     

社会主义市场经济条件下的土地储备及其模式选择

社会主义市场经济建设的逐步完善，要求我们必须改变传统的土地管理思路，土地储备作为政府经营城市的一种重要手段，能够使政府在土地管理的实践中实现资源与资产并重的目标。在阐述土地储备的涵义、起源及其在我国的产生与发展的基础上，分析了土地储备的运行机制与最终目标，归纳了我国土地储备的基本做法，首次提出了城市土地储备的直接储备模式和间接土地储备模式概念，并提出了地方政府构建城市土地储备模式的基本原则和具体操作思路。     

新疆入境旅游市场竞争态分析

提出旅游业市场竞争态模型，运用此模型可以定量分析、对比旅游市场的现实竞争状态，在此基础上，进行市场研究，确定本地区在旅游高超中的地位，明确现在和未来市场竞争的优势和劣势，为地区旅游业发展规划提供参考依据。应用这一模型，分析了新疆入境旅游的客源市场竞争态及省内各地区目的地市场竞争态。     

CELLULOSE DECOMPOSTION IN TROPICAL PEAT SWAMPS

Given that organic soil is a complex substrate and there are many environmental factors which directly or indirectly control its decomposition processes, the use of standard substrate simplify the system in that the effect of substrate quality could be eliminated and influence of certain environmental conditions such as edaphic factors, acidity and moisture could be focused on. In addition to the forest floor, decomposition potential down the peat profile can also be examined. Cotton strip assay was used to estimate decomposition potentials in tropical peat swamp occupied by different Shorea Albida peat swamp forest communities, The 'Alan Batu' , the ' Alan Bunga' , the 'Alan Padang' and the ' mixed Alan' forest communities.Greatest decay rates on the peat surface took place during the wet period. The moist condition of the wet months appeared to favour the growth and stimulate activities of decomposer population and soil invertebrates. Generally, 50% of cotton tensile loss is achieved after four weeks     

土壤水力性质确定方法研究进展

介绍了确定土壤水力性质的各种直接方法(包括实验室方法和田间方法两大类)和间接方法如土壤转换函数方法、分形方法、形态学方法、数值反演方法和经验公式法等的研究进展和现状,指出了它们各自的优点和存在的不足.认为要不断地引入新的实验技术和改进测定设备,并把估计土壤水力性质的各种理论方法及实验信息有机地结合起来进行综合研究,以扩大它们的适用范围和提高预测精度.