Multiple longshore sand bars in the upper Chesapeake Bay

In the upper Chesapeake Bay (Maryland, U.S.A.) field surveys were conducted at 18 multiple longshore sand bar sites. The multiple bar systems were found in water depths less than approximately 2 m (mean sea level), and exhibited mild bottom slopes of 0·0052 or less. The number of bars composing each system ranged from four to 17 and the spacing between the crests typically increased in the offshore direction, ranging from 12 to 70 m. Bar height also typically increased with distance offshore and ranged from 0·03 to 0·61 m. A grain size analysis of crest and trough sediment did not reveal any significant differences and the sediment was categorized as ‘fine sand’. A review of the literature data indicated that the Chesapeake Bay multiple bars possessed similar characteristics to those found in Gelding Bay (Baltic Sea); similarities in fetch, wave height and tidal range between the two bays may account for this finding. The surf-scaling parameter indicated that the multiple bar systems were extremely dissipative with regard to wave energy, and wave height appeared to be an important factor in controlling bar spacing and bar height. A multiple wave break point hypothesis was discussed as a possible mechanism for the formation of Chesapeake Bay multiple longshore bars, and limited observational evidence appeared to support such a mechanism.     

Use of fallout 137Cs for documenting the chronology of overbank sediments from the river Sava, Croatia, and interpreting their geochemical patterns

This paper attempts to determine the impact of former Pb-Zn-Ba mining activities in Slovenian drainage basin on overbank sediments of the Sava river (West Croatia). Among the four studied sequences (S7, S8, S9, S10), the most upstream profile S7 showed the most pronounced impact of ore mining. Mercury was enriched more than 100-fold; Pb and Zn had a strong positive correlation (r > 0.99). Two factor models clearly revealed the Pb-Ba-Hg association in the lower-lying carbonate (CA) population of all four profiles, but only in the overlying silicate (SI) population of profile S7. Factor analysis confirmed this association in the total sediment (90 samples) as well as separately in the CA (33 samples) and SI (57 samples) populations. Based on a comparison with an undisturbed soil profile, the vertical distribution of ¹³⁷Cs in profile S7 was interpreted to be the combination of caesium deposition caused by a flood in 1964 and by post-depositional redistribution.     

溶藻弧菌asp基因在大肠杆菌中表达活性研究及条件优化

为进一步研究溶藻弧菌碱性丝氨酸蛋白酶(ASP)蛋白生物学活性和功能,将构建的pET23d-asp在大肠杆菌BL21(DE3)中表达,对表达产物进行纯化分析,并优化表达条件。结果表明:在咪唑洗脱缓冲液浓度为100 mmol/L时,表达的可溶性ASP被大量洗脱,纯化的ASP相对分子质量约为42 000,具有蛋白活性;在诱导温度28℃、异丙基-β-D硫代半乳糖苷(Isopropyl-β-D-thiogalactopyranoside,IPTG)浓度1 mmol/L及诱导时间16 h时,表达的活性ASP蛋白最多。     

整合多源异构的城市规划数据进行认知交流的研究

针对整个城市规划过程的参与者对规划数据的不同种类、不同层次认知需求,本文利用本体思想对包括GIS数据、规划文档、规划图件等多源异构的规划数据进行关系重构,设计本体模型要素的数据语义关系,定义数据间的关联关系,实现基于本体方法的规划领域语义表达算法,方便了各类规划参与者对规划方案认识的交流:同时根据用户特征分类,进行本体关系的逻辑推理,过滤冗余信息,进行多源异构规划数据的可视化。     

黄河三角洲潮滩剖面特征

利用验潮、潮滩水准测量、沉积物分析和遥感资料,分析了黄河三角洲不同岸段潮滩的特征。水准测量表明,侵蚀潮滩剖面形态为下凹,而淤积潮滩则上凸,由高潮线至低潮线,潮滩沉积物均变粗,这种变粗趋势在北部侵蚀岸段和河口侧缘明显,在河口南侧稳定海岸则不明显。潮滩沉积物含水量为12.2%~32.2%,平均为21%,海滩表层沉积物不排水剪切强度在0.1~0.35 kg/cm2之间。受局限岸滩的平面形态和剖面形态受到周围大坝地形影响,具有和砂质海滩类似的形态,显示波浪对该潮滩的作用强烈,根据剖面测量结果与遥感图像,发现河口北侧有一个小的冲积扇。     

转兔防御素基因小球藻培养的营养模式研究

在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。     

Density profiles and luminosity functions of the globular clusters M3 and M92

Tautenburg Schmidt plates with the globular clusters M3 and M92 were measured on the APM facility in Cambridge, mainly for astrometric purposes. The data were used to derive the radial density profiles and the luminosity functions of these clusters. There is a good agreement of our results with formerly ones derived by other authors. In both clusters effects of mass segregation are observed.     

雄性半滑舌鳎雄激素受体基因的克隆与表达分析

利用RT-PCR方法从半滑舌鳎(Cynoglossus semilaevis)中克隆了雄激素受体(Androgen receptor,AR)基因cDNA的部分序列(434 bp),经BLAST比对与狼鲈(Dicentrarchus labrax)、花溪(Kryptolebias marmoratus)、三斑海猪鱼(Halichoeres trimaculatus)和三刺鱼(Gasterosteus aculeatus)的雄激素受体同源性分别为84%,82%,82%和81%.组织表达分析表明,AR基因在半滑舌鳎的性腺、肝、胃、脾、肾、头肾、肠、鳃、心、脑和肌肉这11种组织中均有表达,表达量有所差异.肾中表达量最丰富,鳃中最少.根据其他硬骨鱼类表达模式,推测半滑舌鳎可能只含有1种AR.     

雨生红球藻八氢番茄红素脱氢酶pds基因上游序列的分离和分析

在某些蓝藻、绿藻和高等植物中,八氢番茄红素脱氢酶是β-胡萝卜素合成过程中的一个关键酶之一,应用巢式PCR方法从雨生红球藻中克隆了八氢番茄红素脱氢酶基因约1kb的5’上游序列。通过生物信息学方法进行序列分析,发现pds基因上游序列中包含了一些可能的顺式元件,如ABRE调控元件、C-repeat/DRE调控元件等。同时,构建了由pds-启动子控制报告基因的重组载体,并转化到雨生红球藻细胞中,进行了报告基因瞬间表达的检测。结果表明,克隆的pds启动子区域具有启动子活性,可以驱使报告基因进行瞬间表达。