黑龙江哈尔滨白渔泡国家湿地公园沼泽、林地和农田土壤物理、化学和生物性质差异

为了揭示黑龙江哈尔滨白渔泡国家湿地公园沼泽、林地和农田土壤物理、化学和生物性质的差异,于2018年7月25日~8月2日,在湿地公园内,在天然芦苇(Phragmites australis)沼泽、林地、旱田和水田中设置采样地,采集不同深度(0~10 cm、10~20 cm和20~30 cm)的土壤样品,测定土壤样品的物理、化学和生物指标。研究结果表明,白渔泡国家湿地公园不同采样地土壤指标存在差异;与天然芦苇沼泽土壤相比,其它采样地土壤的含水量明显偏低,土壤全氮、全磷、碱解氮和有机质含量都明显偏小,水田土壤速效磷含量偏大;天然芦苇沼泽土壤脲酶、硝酸还原酶、纤维素酶、蛋白酶和β-葡萄糖苷酶活性都高于林地和农田土壤,水田0~10 cm和10~20 cm深度土壤的硝酸还原酶活性显著高于旱田和林地;与天然芦苇沼泽土壤相比,旱田土壤小于0.25 mm的小团聚体含量偏大,而其它采样地土壤的各粒级团聚体的比例变化较小,水田土壤团聚体平均重量直径比天然芦苇沼泽和旱田土壤低。     

双酶法制备低黏度辛烯基琥珀酸淀粉钠的工艺条件

对双酶法制备用作微胶囊壁材的低黏度辛烯基琥珀酸淀粉钠的工艺路线与工艺条件进行了研究。正交实验结果表明 ,在酯化淀粉乳质量浓度 2 0 %、每 g淀粉α 淀粉酶的用量为 6SKBU的条件下 ,影响产品性能的各因素中 ,糖化酶的用量对产品的DE值和流度的影响最大 ,各因素的重要性依次是 :糖化酶的用量、糖化酶作用时间、α 淀粉酶保温作用时间。最佳工艺条件是 :α 淀粉酶保温作用时间 4min ,每 g淀粉糖化酶用量 0 .2GAU ,糖化酶作用时间 2 .5h ,此时产品的DE值为 30 ,流度为 80左右     

Environmental contamination of Zn,Cd, Ni,Cu, and Pb from industrial areas in Hamadan Province,western Iran

Eleven surface soil samples from calcareous soils of industrial areas in Hamadan Province, western Iran were analyzed for total concentrations of Zn, Cd, Ni, Cu and Pb and were sequentially extracted into six fractions to determine the bioavailability of various heavy metal forms. Total Zn, Cd, Ni, Cu and Pb concentrations of the contaminated soils were 658 (57–5,803), 125.8 (1.18–1,361), 45.6 (30.7–64.4), 29.7 (11.7–83.5) and 2,419 (66–24,850) mg kg⁻¹, respectively. The soils were polluted with Zn, Pb, and Cu to some extent and heavily polluted with Cd. Nickel values were not above regulatory limits. Copper existed in soil mainly in residual (RES) and organic (OM) fractions (about 42 and 33%, respectively), whereas Zn occurred essentially as RES fraction (about 69%). The considerable presence of Cd (30.8%) and Pb (39%) in the CARB fraction suggests these elements have high potential biavailability and leachability in soils from contaminated soils. The mobile and bioavailable (EXCH and CARB) fractions of Zn, Cd, Ni, Cu, and Pb in contaminated soils averaged (7.3, 40.4, 16, 12.9 and 40.8%), respectively, which suggests that the mobility and bioavailability of the five metals probably decline in the following order: Cd = Pb > Ni > Cu > Zn.     

Regional distribution pattern of groundwater heavy metals resulting from agricultural activities

Contaminations of groundwater by heavy metals due to agricultural activities are growing recently. The objective of this study was to evaluate and map regional patterns of heavy metals (Cd, Zn and Cu) in groundwater on a plain with high agricultural activities. The study was conducted to investigate the concentration of heavy metals and distribution in groundwater in regions of Shush Danial and Andimeshk aquifers in the southern part of Iran. Presently, groundwater is the only appropriate and widely used source of drinking water for rural and urban communities in this region. The region covers an area of 1,100 km² between the Dez and Karkhe rivers, which lead to the Persian Gulf. For this study, the region was divided into four sub-regions A, B, C and D. Additionally, 168 groundwater samples were collected from 42 water wells during the earlier months of 2004. The flame atomic absorption spectrometry (AAS-Flame) was used to measure the concentration of heavy metals in water samples and the Surfer software was used for determination of the contour map of metal distribution. The results demonstrated that in all of the samples, Cd and Zn concentrations were below the EPA MCLG and EPA secondary standard, respectively. However, the Cu contents of 4.8 % of all samples were higher than EPA MCL. It is also indicated that the concentrations of metals were more pronounced at the southern part of the studied region than at the others. The analysis of fertilizers applied for agricultural activities at this region also indicated that a great majority of the above-mentioned heavy metals were discharged into the environment. Absence of confining layers, proximity to land surface, excess agricultural activities in the southern part and groundwater flow direction that is generally from the north to the southern parts in this area make the southern region of the Shush plain especially vulnerable to pollution by heavy metals than by other contaminants.     

冲绳海槽中段沉积岩芯碳酸盐和烧失量的古环境意义

对冲绳海槽中段 3个沉积岩芯碳酸盐和烧失量的系统分析得知 ,岩芯沉积物的CaCO3含量一般 >1 0 % ,最高含量接近 30 % ,烧失量大都在 1 0 %～ 30 %之间变化 ,反映了该海区处于溶跃面之上钙质生物极为发育的生物地球化学环境。结合δ1 8O曲线所反映的气候环境变化 ,探讨了 1 2 0kaB .P .以来碳酸盐旋回的“大西洋型”演变特点 ,同时对该区沉积地层中的火山热液活动印记作了一定的探讨。     

中国对虾(Penaeus chinensis)消化系统发生的研究

本文对中国对虾消化道和中肠腺的发生进行了研究。完整的中肠管形成于Ｎ３期，它是由幼虫腹面的内胚层板向背中线上卷合拢而形成。在Ｚ１期消化道完全贯通。至Ｍ１期，胃分化为贲门胃和幽门胃两部分，中肠前盲囊和中肠后盲囊也在此期出现，同时胃磨的雏形发生，但完整的胃磨形成于Ｐ期。另外，中肠腺始于Ｎ４－５期中肠前端突出的两对中肠腺盲囊。第一对中肠腺盲囊于Ｍ３期退化消失，而第二对中肠腺盲囊则发育为成体的中肠腺。中肠腺小管由４种不同类型的细胞（胚性细胞、吸收细胞、纤维细胞和泡状细胞）所组成     

大黄鱼病原弧菌拮抗放线菌的筛选与人工诱变

从厦门集美海滩底泥分离得到38株放线菌，用琼脂块法测定它们对大黄鱼病原弧菌-溶藻弧菌和副溶血弧菌的拮抗效果，选择两株拮抗效果较好的放线菌进行诱变，测定诱变后各菌株的抗菌效果，并选择抗菌效果较好的菌株进行第二次诱变，如此反复诱变3次，共得到97株放线菌，结果表明；38株放线菌中的22株对两株病原菌有一定的拮抗作用；用紫外线照射可以获得少量对两株病原菌拮抗作用加强的菌株。     

酶解条件对条斑紫菜单细胞成活率影响的比较研究

该文分别选用不同浓度的海螺酶 ;葡萄糖、蔗糖、甘露醇、山梨醇、氯化钠 5种渗透剂 ;维生素 C和甘露醇 2种抗氧化剂 ;对条斑紫菜酶解单细胞的成活率进行了研究。实验结果表明 :用浓度为 10 %的海螺酶酶解条斑紫菜 ,成活率最高 ;选用 2 mol/ L葡萄糖作渗透剂 ,效果最好 ,成活率达85.2 % ;加入抗氧化剂对细胞成活率没有影响     

产复合酶菌株Pseudomonas sp.NJ197产酶发酵条件的研究

从南极普利兹湾深海900m的沉积物中筛选到一株同时产多种低温复合酶的菌 株NJ197,作者对其进行碳源、氮源、无机盐、起始pH值、培养时间、接种量等发酵条 件的优化实验．实验结果证明,在以0．5％可溶性淀粉为碳源,以0．5％豆饼粉为氮 源,无机盐:0．424％NH4H2PO4、0．075％K2HPO4、0．02％MgSO4、0．5％CaCO3,起始 pH值8．5,接种36h种龄的种子10％,20℃、250r／min的条件下在旋转摇床中培养 72h,产复合酶菌株产脂肪酶和淀粉酶活性最高．最佳的生理条件下复合酶中的碱性 脂肪酶和淀粉酶的产量分别提高到22．4U／cm3和30．9U／cm3,该产复合酶菌株可以 作为诱变育种、基因工程菌改造的出发菌株,在洗涤和制革工业中有良好的应用前 景．     

莫桑比克罗非鱼孵卵期间口腔粘膜变化的研究

用组织学、组织化学和酶技术,对雌性莫桑比克罗非鱼在孵卵前后口腔粘膜的组织结构、抗菌酶的比活力变化进行初步研究。结果表明:在口腔粘膜的上皮中,杯状细胞是一个重要的结构,它随着雌鱼的生理状态的改变而变化。雄鱼、雌鱼和孵卵期的雌鱼口腔粘膜上皮中的杯状细胞数量上的差别显著。孵卵期的雌鱼口腔粘膜类似于哺乳动物的子宫粘膜也是受性激素影响的靶器官。该种鱼的口腔粘膜中存在着两种抗菌酶;蛋白质水解酶和壳多糖酶。孵卵前后,这两种酶的比活力分别提高1.4—2.03倍。说明孵卵时,口腔粘膜为使卵及仔鱼健康的发育,增强了抗菌能力。研究结果证明:抗菌力的增加与口腔粘膜中的杯状细胞的大量增生有着密切的关系。