光裸方格星虫增养殖对滩涂底质的影响

基于2016年春、秋两个季节的现场实验数据, 研究光裸方格星虫(Sipunculus nudus Linnaeus)滩涂增养殖对滩涂底质的影响。实验对方格星虫单养区(S组)、方格星虫-贝类混养区(P组)和无星虫对照区(C组)进行了有机质、粒径组成、氧化还原电位(ORP)等指标测定, 结果表明: 1) 秋季S组20~30cm层次有机质含量和细沙比例均显著高于春季, 而C组仅表层物质有所增加, 表明方格星虫具有较强的表层物质迁移能力, 且其迁移深度为20~30cm。2) 秋季C组各层氧化还原电位值(ORP)均无明显降低趋势, 而S组仅10~20cm层次有一定程度降低, 表明方格星虫增养殖并不会明显降低滩涂底质状况。3) P组各层有机质以及细沙含量增加, 表明皱肋文蛤的过滤沉积作用可以提高滩涂颗粒物质的积累, 且方格星虫混养会加速颗粒物质的向下迁移; P组皱肋文蛤的栖息层次(0~3cm、3~10cm) ORP明显低于S组(p<0.05), 表明高密度的皱肋文蛤代谢活动会加剧0~10cm的底质恶化。4) 方格星虫与皱肋文蛤具有生态互补作用, 在同一区域进行增养殖可以提高滩涂物质利用率及碳汇功能; 方格星虫扰动引入富氧水可以缓解皱肋文蛤栖息层次的底质。     

浮床无土栽培植物控制池塘富营养化水质

采用浮床无土栽培技术,在池塘水面种植景观植物--陆生植物美人蕉(Cana generalis     

暗纹东方鲀温室养殖技术及其经济效益分析

利用养鳗温室养殖暗纹东方,经过一年半的养殖,共养殖商品河鱼5272．5公斤,每平方米生产河鱼2．3公斤,成活率达72．8％,盈利64．88万元,取得了较好的经济效益。同时,总结了一套较为有效的温室养殖技术。     

两种沉水植物对黄颡鱼(Pseudobagrus fulvidraco)夏花培育水体主要水质因子的影响

以黄颡鱼夏花培育水体为实验用养殖污水,伊乐藻、轮叶黑藻作为净化水质的沉水植物材料,建立封闭型(非换水)和交换型(定期换水)的两种鱼草共生的生态系统并与传统的商业性养殖系统模式作同步比较,分析了养鱼水体水质主要因子的变化及对鱼存活率的影响．实验结果如下：1．作为实验组的鱼草共生系统两种水体水质优良,鱼类生长良好,交换型水体中D0值≥8．0,NH4—N≤0．34,COD≤18mg/L,悬浮物≤12mg/L夏花成活率为93．3％;封闭型水体中D0值≥7．5,总NH4—N≤0.92,COD≤28mg/L,悬浮物毒20mg/L夏花成活率为60．0%．2．作为对照组有色无草的封闭系统中水质逐渐恶化,鱼类生长受到抑制甚至生存也不能保障,其中,D0值≥2．5,NH4-N≤3.22,COD≤88mg/L,悬浮物≤55mg/L,夏花成活率仅为6．7％;交换系统中D0值≥4．0,总NH4—N≤2．41,COD≤66mg/L,悬浮物≤51mg/L,即使定期换水夏花成活率也仅达66．7％;研究结果表明：鱼草共生系统中栽培伊乐藻、轮叶黑藻等沉水植物可有效地净化水质,不仅确保了夏花生产良性运行,还节约了水资源并达到无污染排放．     

A Multi-Generation Schmakeriapoplesia Culturing System for Use in Ecotoxicological Study

Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary, ecosystems. We have established a multi-generation cultivation system for zooplankton ruth which future experiments on the biological effects of pollutants in marine and estuary environments can be better performed. A population of calanoid copepod, Sehmakeria poplesia, was collected in December 2003 and maintained in a static system through all stages （eggs to adults）. The population ex- hinted an average developmental time of 13.6 d in conditions corresponding to the natural environment （water temperature 20~C salinity 15）. A series of experiments were performed to examine copepod egg production and hatching success as functions of food type and feeding concentration. Results in our study showed that Isoehrysis galbana was more favored for the reproduction of cope- pods than Phaeodactylum tricornutum, and 10xl0%ellsmL-1 was the most practical algae concentration. We have demonstrated that the Schmakeria poplesia population can be maintained in the laboratory through multiple generations. In addition, methods to control egg production through changes in food concentration have been established, making it feasible to control the start date of exposure experiments or the timing of the collection of oftspring to initiate a new generation.     

A multi-generation Schmakeria poplesia culturing system for use in ecotoxicological study

Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary ecosystems. We have established a multi-generation cultivation system for zooplankton with which future experiments on the biological effects of pollutants in marine and estuary environments can be better performed. A population of calanoid copepod, Schmakeria poplesia, was collected in December 2003 and maintained in a static system through all stages (eggs to adults). The population exhibited an average developmental time of 13.6 d in conditions corresponding to the natural environment (water temperature 20°C, salinity 15). A series of experiments were performed to examine copepod egg production and hatching success as functions of food type and feeding concentration. Results in our study showed that Isochrysis galbana was more favored for the reproduction of copepods than Phaeodactylum tricornutum, and 10×10⁴cells mL⁻¹ was the most practical algae concentration. We have demonstrated that the Schmakeria poplesia population can be maintained in the laboratory through multiple generations. In addition, methods to control egg production through changes in food concentration have been established, making it feasible to control the start date of exposure experiments or the timing of the collection of offspring to initiate a new generation.     

生物磁性纳米材料——磁小体培养优化及形成过程研究

采用正交试验的方法优化磁小体培养条件,结果使趋磁细菌OD600达到0.440(生物量为1.166×109cells/ml),同时运用磁收集传代法,使趋磁细菌Cmag值稳定在1.9—2.0。培养时用透射电镜观察磁小体形成过程,结果发现培养24h细菌体内已有较小晶体形成且沿长轴分布;48h晶体长大且形成分段链沿长轴排列;72h晶体进一步成熟仍以分段链沿长轴排列;随后细菌逐渐衰亡磁小体变小,168h可见部分自溶细菌中仍有磁小体链;192h细菌自溶磁小体链分散到环境中。还观察到磁小体在细菌分裂时采用两种分离方式,一种为磁小体分配到两个子细胞;另一种为磁小体只分配到一个子细胞。无磁小体的子细胞,一部分逐渐产生磁小体,另一部分不产生磁小体。     

缩短栉孔扇贝养殖周期新措施的探讨

本文主要介绍了利用栉孔扇贝三倍体，升温苗的养成及强化养成管理等新措施来达到缩短栉孔扇贝养殖周期，并取得了较好的效果。     

羊栖菜幼孢子体苗海区培育的研究

在不同的海区培育羊栖菜的幼孢子体苗，研究不同时间下海的幼苗在不同海区、不同的水层生长与附着情况，再结合海区的生态因子的分析观察，表明羊栖菜的幼孢子体苗可以在浙江海区培育成为羊栖菜栽培的种苗，10月下旬前后下海培育比较合适，小于透明度的培养水层是适宜的，平挂的效果优于吊挂。     

马氏珠母贝二次插核的研究

为探索珍珠培育的新途径 ,提高珍珠养殖的经济效益 ,利用马氏珠母贝(PinctadamartensiiDunker)进行第二次插核育珠 ,结果表明 :二次插核珠的珍珠层6个月就达418μm±105μm ,明显高于对照组 ,且优质珍珠的产生率达40% ,育珠贝的存活率达95% ,在每只贝插一珠核的情况下留核率仍高达0.95 ,二次插核对马氏珠母贝的重复利用率为34%。因此 ,马氏珠母贝二次插核培育珍珠技术 ,能重复利用贝体 ,缩短育珠期 ,提高珍珠的产量和质量。