两种江蓠果孢子的融合研究

本研究以真江蓠(Gracilaria asiatica Zhang et Xia)和龙须菜(Gracilaria sjoes-tedtii Kylin)两个不同物种为材料,探索了果孢子的融合方法和条件。由成熟囊果的藻体经阴干刺激后,放散出大量果孢子。两个物种的果孢子在大小和颜色上都有差异。对这两个物种的果孢子用PEG—高Ca~(++)高pH法诱导融合,首次获得成功,融合细胞能再生成杂种植株。     

温度、光照强度和盐度对海柏果孢子放散与附着的影响

在实验室条件下,研究了不同温度、光照强度和盐度对海柏(Polyopes polyideoides)果孢子的放散及附着的影响。结果表明:温度10~26℃的条件下,海柏果孢子均能够完成放散附着;18℃时,海柏果孢子放散附着速度最快且总量最大,而当温度低于5℃或者高于30℃时,海柏果孢子的放散附着受到抑制,几乎不放散附着;海柏果孢子放散附着的适宜光照条件为80?mol/(m2·s),光照强度过高时海柏果孢子的放散附着明显受到抑制;盐度为22~36之间利于果孢子的放散附着,当盐度低于8,高于40的时候,海柏果孢子由于受到了盐胁迫,几乎不放散及附着。     

Early development of grateloupia turuturu (Halymeniaceae, Rhodophyta)

Grateloupia turuturu is a commercial red alga with potential value in nutraceuticals and pharmaceuticals. To supplement information on its life history and verify whether carpospores can be used for seedling culture, early development of G. turuturu was investigated under culture conditions (27°C, 10-13 mol/(m2.s) in irradiance, photoperiod 10:14 h L:D). Three physiological stages were recognized by continuous microscopic observation: division stage, discoid crust stage, and juvenile seedling stage. At the beginning of the division stage, the carpospores developed germ tubes into which the carpospore protoplasm was evacuated, and then the carpospore protoplasm in the germ tubes began to divide continuously until discoid crusts formed. Finally, upright thalli appeared on the discoid crusts and developed into juvenile seedlings. It took about 60 days for carpospores to develop into juvenile seedlings. The growth parameters, including germination rate for carpospores and discoid crust diameter, were recorded. These results contribute more information on the life cycle, and at the same time are of great significance in the scaling-up of artificial seedling cultures of G. turuturu.