中国对虾血细胞酶细胞化学的初步研究

运用电镜酶细胞化学技术 ,对中国对虾 (Penaeus chinensis)的血细胞和血清中的酚氧化酶、酸性磷酸酶、碱性磷酸酶和过氧化物酶的酶活性部位进行标记。实验结果显示 :酚氧化酶主要以酶原形式存在于大颗粒细胞的颗粒体中 ,酸性磷酸酶和碱性磷酸酶以酶原形式存在于小颗粒细胞的颗粒体中。健康虾和患病虾血细胞中酶活性的表现形式不尽相同 ,在病理情况下 ,颗粒细胞能以细胞解体的方式 ,排出细胞内的颗粒 ,转化成有活性的酶 ,以此提高血淋巴中这几种酶的活性。透明细胞可以吞噬排放颗粒后的颗粒细胞 ,透明细胞中由高尔基体合成的酸性磷酸酶等溶酶体酶主要用于透明细胞的细胞内消化作用。研究认为 :3种血细胞因含有不同的免疫酶以及酶的作用方式不同而具有不同的免疫功能     

Control strategies for the Clam Wave Energy Device

A promising wave energy device being currently investigated is the ‘clam’. The clam extracts energy by pumping air through a specially designed (Wells) turbine. Although operation of the Wells turbine does not require a rectified air flow, some additional control will be necessary to optimize the phase of the clam motion for good efficiencies. An examination of the equation of motion in the time domain suggests the possibility of phase control by mechanical, power take-off, or pneumatic latching. Latching can be shown to increase the efficiency of the device in the longer wavelengths of the wave spectrum, i.e. those of high incident wave power. Equivalently latching could be used to keep the device efficiency high while reducing its size, possibly resulting in cheaper power extraction.     

Effects of tributyltin at environmental levels on monooxygenase system of digestive gland in hard clam Meretrix meretrix

The effects on ethoxyresorufin O-deethylase （EROD）, NADPH-cytochrome c reductase and NADH cytochrorne b5 reductase activities of digestive gland in Meretrix meretrix exposed to tributyltin （TBT） at environmental levels （0.1,1.0,10.0 ng/dm as stannum concentration）,in experimental condition, were evaluated. The EROD, NADH cytochrorne b5 reductase activities were significantly inhibited after exposure to 10.0 ng/dm^3 TBT for 8 and 20 d, the NADPH cytochrorne c reductase activities were significantly inhibited after exposure to 0.1,1.0 and 10.0 ng/dm TBT for 8 d and to 1.0 and 10.0 ng/dm for 20 d, as compared with the matched control, while NADH cytochrorne b5 reductases and NADPH cytochrome c reductase activities were induced after exposure to 10.0 ng/dm^3 TBT for 2 d. The EROD activity in the 10 ng/dm^3 group,and the NADH cytochrome b5 reductases activities in 1.0 and 10.0 ng/dm groups, were significantly induced when transferred to clean recovery tanks for 7 d. The three enzymic activities in the clams exposed to TBT were recovered to the level corresponding to that of the control group after transfer to clean recovery tanks for 20 d. NADPH cytochrome c reductase activity in Meretrix meretrix seems to be more sensitive to exposure of TBT than that of the EROD and NADH cytochrome b5 reductases. The results suggest that induction and inhibition by TBT to the monooxygenase system enzymic activity in Meretrix meretrix would simultaneously exist. The enzymic activities were inhibited by exposure for a long time. The results suggest that inhibition of the monooxygenase system should be an indication of the exposure to environmentally relevant concentrations of TBT for a long time.     

Heavy metal contents in soil of major towns in the east coast of Peninsular Malaysia

A total of 63 soil samples from 3 different soil profiles （urban, suburban and industrial areas） in major towns in the east coast of Peninsular Malaysia were analyzed for the total concentrations of Cu, Zn, Pb, Ni and Cr. The soil samples were subjected to acid digestion and the concentrations of total metals extracted were measured or flame atomic absorption spectrometry and inductively coupled plasma - atomic emission spectrometry. According to the result of this study, Pb and Zn concentrations in urban soils are much higher than those of industrial and suburban soils. Total concentrations of Cu and Cr in industrial soil samples are high compared to other two soil profiles and Ni concentrations in the suburban area are slightly higher those of urban and industrial soils. Since Malaysia has not yet to come up with her own soil maximum allowable limit, the heavy metal concentrations were compared with the Dutch maximum allowable limit. The results indicated that the median of heavy metals values in the three different soil profiles is still below the Dutch system limit. From the maximum allowable value obtained from the Dutch system, a contamination/pollution （C/p） index for each site was calculated for the set of these five heavy metals. An advantage of using this method is to make a differentiation between pollution （C/p〉1） and contamination （C/p〈1） status in soils as well as being able to characterize each status into 5 different categories （slight, moderate, severe, very severe and excessive）.     

Changes in manganese and lead in the environment and young children associated with the introduction of MMT in gasoline

This is a 4-year longitudinal study to evaluate changes to the environment and exposure of young children associated with the introduction of methylcyclopentadienyl manganese tricarbonyl （MMT） into Australia in 2000. The cohort includes 57 females and 56 males; age range of 0.29 to 3.9 years. Samples are collected every 6 months from children in residences located at varying distances from major traffic thoroughfares in Sydney. Environmental samples： air, house and day care dustfall, soil, dust sweepings and gasoline. Samples from children： blood, urine, handwipes prior to and after playing outdoors, and a 6-day duplicate diet. All samples are analyzed for a suite of 20 elements using ICP-MS. Results are presented for the first three 6-month sampling periods for Pb and Mn. For dustfall accumulation, there was no significant change over the 3 sampling periods （time） for Pb or Mn, and a positive relationship between ‘traffic exposure＇ （traffic volume and proximity to the road） and Pb but not Mn. For handwipes, Pb and Mn in wipes taken from children after playing outdoors were usually significantly greater than for wipes taken prior to playing. There was no significant association between Pb or Mn in handwipes with traffic exposure. Dustfall accumulation was a significant predictor for Pb in the handwipes, and dust sweepings were a significant predictor of Mn in handwipes.     

Toxicities of p-nitrotoluenes and their effects on blood corpuscles and ovaries in Crusian carps (Carassius auratus)

p-nitrotoluene is an important organic intermediate widely used in pesticide, foamed plastics, dyestuff and medicine industries. In this paper, Crusian carps (Carassius auratus) were exposed to dilute p-nitrotoluene solutions with different concentrations of 0, 20, 40, 80, 120, 160, 240 and 320 μg/L; the subsequent physiological responses to the chemical were observed, and the variation characteristics of blood corpuscles, ovaries and oocytes were studied by bio-microscopy. Results showed that even very low concentrations of p-nitrotoluene would lead to toxicosis and even the death of Carassius auratus. The bio-microscopic structures revealed that, compared with the compartment of Carassius auratus growing in the p-nitrotoluene-free water (the control group), the lower concentrations of p-nitrotoluene (for example no more than 80 μg/L) would result in the faster disintegration of erythrocytes and the significant enlargement of oocytes, while the higher contents of p-nitrotoluene (no less than 240 μg/L in this case) would inhibit the division of cells and their further growth, even degenerate blood corpuscles and ovaries, suggesting stronger estrogenic activities. The study is financially supported by the Development Foundation of Science and Technology under Shandong Provincial Education Department (No. J05D51).     

南昌市呼吸道和脑血管疾病与天气类型关系的初步分析

使用南昌市4家大医院(江西医学院第二附属医院、江西省肺科医院、南昌市第一医院、南昌市中西医结合医院)1999年呼吸道和脑血管两大类常见疾病的住院病例数,挑选出发病高峰期,对照相应日期的天气图进行分析,发现夏季的低气压和其它季节的冷空气是诱发或加重呼吸道和脑血管疾病的主要因素,气温或气压的变化对疾病的诱发或加重具有一定的滞后性。     

In situ sampling reveals rapid uptake and depuration of polycyclic aromatic hydrocarbons by surf clams (Paphies subtriangulata) affected by the Rena oil spill

ABSTRACT

To investigate the uptake and depuration of polycyclic aromatic hydrocarbons associated with the Rena oil spill we sampled the surf clam Paphies subtriangulata at two open coast locations (6?km apart) just prior to oil coming ashore (7 October 2011), then at 1–3 week intervals for the next 4 months. Total polycyclic aromatic hydrocarbons (tPAH) increased at both sites from 1 to 96–124?µg?kg^?1 (wet weight) by 18 October before declining to low levels (<4?µg?kg^?1) by February 2012. Ongoing sampling throughout 2012–2014 included three additional sites to the north east (up to 30?km away) and a site 5?km to the south east revealing tPAH levels generally <10?µg?kg^?1 except in October 2013 where levels ranged between 39–45?µg?kg^?1 at all sites. A comparison of PAH component profiles with oil-contaminated beach sediment indicated that the high levels observed in surf clams between October–December 2011 were clearly associated with the Rena spill. However, the October 2013 peak had a PAH profile inconsistent with weathered Rena oil, suggesting an alternative source of contamination. Our results highlight the potential for P. subtriangulata as a PAH monitoring tool but recognise more study is needed to better quantify baseline levels and uptake and depuration dynamics.     

黑鱾血液的生理生化指标研究

本文采用F-820半自动血细胞分析仪和全自动生化分析仪,对黑纪的血液常规指标及血清中的17项生化指标进行了分析测定,并用Wright’s染色法制作血涂片对白细胞进行分类计数．结果表明：（1）黑纪的血红细胞数量为（2．49±0．47）×10^12个／dm^3;白细胞数量为（1．08±0．21）×10^11个／dm^3;在白细胞中以嗜中性粒细胞数量最多,占52．42％±4．67％,其次为淋巴细胞,占25．72％±2．88％;单核细胞体积最大,占总数的3．27％±0．62％．（2）黑纪红细胞脆性大,抗性差,在0．65％～0．70％的NaCl溶液中开始溶血,在0．50％～0．55％的NaCl溶液中完全溶血．（3）黑纪的血红蛋白（HGB）为109．11±15．72g／dm^3,高于一般鱼类,与其较强的耐低氧生理能力相适应．（4）血清中血糖浓度为4．72±1．35mmol／dm^3,明显低于凶猛肉食性鱼类,与其岛礁性生态习性和杂食性相吻合．（5）雌雄鱼的乳酸脱氢酶分别为332．67±188．48U／dm^3和905．66±257．85U／dm^3,雌鱼显著高于雄鱼,可能与繁殖活动相关．     

天津沿海菲律宾蛤仔人工育苗技术的初步研究

利用自然海水和天然饵料,采用无泥砂采苗方法,在天津沿海首次成功开展了菲律宾蛤仔工厂化育苗。幼虫初始壳长93.8μm,壳长平均日增长8.3μm,成活率41.0%;第15-23 d为变态期,壳长平均日增长12.9μm,变态率47.8%;变态后至第55 d,壳长平均日增长33.2μm,成活率76.0%。本次育苗试验充分利用天津沿海水质条件与环境特点,改进育苗方法,降低生产成本,建立了适于天津海区的菲律宾蛤仔人工育苗工艺,共得到双水管稚贝约1 800万枚。