厦门港浮游植物对磷酸盐吸收速率的研究

用无载体32P同位素示踪测定海水中浮游植物和细菌吸收磷酸盐速率的方法，对厦门港浮游植物和细菌进行了研究。结果表明，厦门港海水中吸收磷酸盐的主体是浮游植物（几乎占100％），平均吸收速率为4．28×10－5μmol／（L·s），并且存在着晚秋＜冬＜初春的季节变化，培养实验的结果表明吸收速率的季节变化主要是由于种类更替和温度效应所致。     

Transient biouptake flux and accumulation by microorganisms: The case of two types of sites with Langmuir adsorption

The uptake of a chemical species by an aquatic microorganism is modelled considering two kinds of sites where Langmuirian adsorption is followed by first order internalisation kinetics. Simpler models, such as only one internalisation route (while most of the adsorption takes place on non-internalising sites) or a linear isotherm for adsorption on one or both sites, become limiting cases of this double-Langmuirian model. The model considers the sites located on the spherical (or semi-spherical) surface of the organism, and takes diffusion from the medium into explicit account. The numerical solution for the internalisation flux shows a maximum. We provide an estimate for the time needed to reach a certain proximity to steady state. The transient solution confirms that the analytical expressions for the steady-state flux are usually valid and that the accumulated amounts reflect the impact of the short-time uptake. The Instantaneous Steady-State Approximation (ISSA), where an intercept of the linear regression of accumulated amount as a function of time is interpreted as an adsorbed amount, can be critically assessed with the transient numerical code for two cases: (i) when the total burden of metal on the cell is the input data and (ii) when an extraction procedure provides further information on the adsorbed and internalised amount.     

Bioconcentration of selected endocrine disrupting compounds in the Mediterranean mussel, Mytilus galloprovincialis

The occurrence of three endocrine disrupting compounds, 4-n-nonylphenol, triclosan and bisphenol A, was investigated in different bivalves originating from the Aegean Sea (Greece). The bioconcentration potential of these compounds was studied using the Mediterranean mussel (Mytilus galloprovincialis). Tissue samples were extracted by sonication. Analysis was performed by gas chromatography-mass spectrometry. According to the field survey results, the average concentrations of 4-n-nonylphenol, triclosan and bisphenol A, were 158, 461 and 404 ng g⁻¹ (dry weight), respectively. During 28 days of exposure at 300 ng L⁻¹, the tissue concentrations of compounds were constantly increased. Steady state was not observed up to the end of the experiment. Kinetic bioconcentration factors varied from 1.7 (4-n-nonylphenol and triclosan) to 4.5 L g⁻¹ (bisphenol A). Following exposure, mussels were relocated to clean water for 28 days. This experiment revealed that depuration rates for all of the target compounds were lower than uptake rates. The biological half-lives of each compound ranged between 12 days (triclosan) and 26 days (bisphenol A).     

Uptake of platinum group elements by the marine macroalga, Ulva lactuca

Uptake of environmentally relevant platinum group elements (PGE) by the marine macroalga, Ulva lactuca, has been studied. Removal of nM concentrations of Rh(III), Pd(II) and Pt(IV) added to filtered sea water appeared to proceed via pseudo-first-order kinetics, with respective forward rate constants of either 0.0039 or 0.0042 h^− 1, 0.0058 or 0.0096 h^− 1 and 0.0017 or 0.0032 h^− 1, depending on whether an irreversible or reversible reaction was invoked. The (quasi-) equilibrium distribution coefficients, derived from linear fits to uptake (sorption) isotherms, were about 1400, 900 and 350 mL g^− 1 on a dry mass basis for Rh, Pd and Pt, respectively. With increasing sea water pH, over the range 7.9 to 8.4, uptake of Rh by Ulva increased considerably, whereas a small increase in Pt removal was observed; in contrast, uptake of Pd exhibited no clear dependence on pH. The percentage of metal taken up that was internalised within cells, evaluated by washing selected algal samples in 3 mM EDTA, was about 40% for Rh, 80% for Pd and 95% for Pt. Results of this study were interpreted in terms of what is known about the aqueous speciation of PGE in sea water. Thus, Rh exists as cationic hydrated chloride complexes which are readily adsorbed at the algal surface. Palladium has an exceptional affinity for organic ligands, and uptake (and internalisation) appears to be governed by competition for Pd²⁺ from aqueous and algal binding sites. Platinum (IV) exists predominantly as a series of (mainly) negatively charged chloride and mixed hydroxychloride complexes that have little propensity to interact with the algal surface; however, its high degree of internalisation requires at least some interaction with specific and perhaps physiologically active sites.     

Inorganic and organic nitrogen cycling in the Southern California Bight

On the basis of mass balance calculations performed for nitrogen (N) uptake experiments in the Southern California Bight (SCB), it has been suggested that a significant portion of dissolved inorganic N (DIN) uptake results in the production of dissolved organic N (DON). To investigate this process, the fate of ammonium (NH₄⁺) and nitrate (NO₃⁻) uptake was quantified within the euphotic zone at three coastal stations in the SCB using ¹⁵N tracer techniques. Several trends in the fate of DIN and the production of DON were observed. First, production of particulate N (PN), from both NH₄⁺ and NO₃⁻, was quantitatively more important in near surface waters, while DON release dominated within the nitracline. Second, the percentage of gross N uptake released as DON was generally higher when NO₃⁻, rather than NH₄⁺, was the substrate. Third, the percentage of N released as DON was higher at night, relative to the day. Fourth, rates of DON release were significantly correlated to NH₄⁺ regeneration, suggesting that similar mechanisms are responsible for both processes—presumably grazing. The results of this study indicate that the DON pool is a sink for DIN uptake on the time scale of hours. One implication of this finding is that new production estimates based on ¹⁵NO₃⁻ uptake rates will likely underestimate particle flux out of the surface layer because the rate of NO₃⁻ uptake is underestimated due to loss of DO¹⁵N during the incubation. On time scales of months to years, however, the N that is taken up as NO₃⁻ and released as DON will likely contribute to export flux via incorporation of the dissolved phase during seasonal mixing into sinking particles or transport. The export of DON on these time scales argues for the use of gross uptake rates to calculate f-ratios.     

Forest fire ash impact on micro- and macroalgae in the receiving waters of the east coast of South Korea

Mountain forest fire ash flushed into the eastern coastal waters of South Korea is known to contain cadmium as one of its significant constituents. To study its impact, two representatives of the micro- and macroalgal communities, Ulva pertusa and Nannochloropsis oculata, were exposed to the concentration range of Cd indicated from the forest fire localities. At low concentrations of 0.224–0.448 mg L⁻¹, a 20% reduction in growth rate of N. oculata was observed. Chlorophyll a pigment concentration was proportionate to the growth reduction while at higher concentrations (1.792 mg L⁻¹) pigments were completely leached. In the macrophyte U. pertusa, a similar trend of pigment leaching was observed. Bioaccumulation factors obtained for these rapidly growing algal species revealed excessive bioconcentration of Cd. Variations in the concentration of Cd among the samples collected along the Korean coast clearly indicated the additional source of metal influx from the forest fires.     

Organotin accumulation in an estuarine food chain: comparing field measurements with model estimations

The bioaccumulation model OMEGA (optimal modelling for ecotoxicological applications) is used to explore accumulation of organotins in the Western Scheldt food chain, consisting of herbi-detritivores, primary and secondary carnivorous fish and a piscivorous bird. Organotins studied are tributyltin (TBT) and triphenyltin (TPT) and the respective di- and mono-organotin metabolites. Empirical elimination rate constants are compared to model predictions for organic substances and metals. It is found that field bioaccumulation ratios are higher than predicted based on elimination kinetics relevant for organic compounds. The results indicate that uptake of organotins mainly occurs via hydrophobic mechanisms, whereas elimination may occur via metal-like kinetics. This results in very low elimination rates, which are comparable to model predictions for metals.     

菊花江蓠(Gracilaria lichenoides)对N、P吸收效应及其细胞超微结构变化

通过设置5个N/P浓度梯度和3个N浓度下的4个N:P梯度,进行了江蓠对N、P的短期(O-4h)和长期(第14天)吸收速率(SNUR和LNUR)以及短期和长期吸收效率(SNUE和LNUE)的研究,同时观察了5个N/P浓度梯度对江蓠细胞超微结构的影响.结果表明:N∶P比为16∶1时,江蓠对N、P的SNUR和LNUR均最大(P＜0.05); N/P为1200/75和960/60μmol/L浓度时,分别对N和P的SNUR最大,达12.85和0.79μmol/(gDW·h),但此N/P浓度下长期培养的江蓠叶绿体结构存在不同程度的损伤;而N/P浓度480/30μmol/L时,对N和P的LNUR均最大值4.23和0.31 μmol/(gDW·h).江蓠对N、P的SNUE和LNUE在介质N/P 60/3.75μmol/L时为最高,而在1200/75 μmol/L时为最低(P＜0.05).     

贺兰山地区沙尘暴沙尘起动和垂直输送物理因子的综合研究

根据 4a来在贺兰山两侧沙漠和沙地测站积累的微气象和沙尘谱观测资料 ,结合有关文献 ,综合分析了沙尘起动和垂直输送的物理机制 ;讨论了强风的动力作用、近地面不稳定层结的热力作用和沙尘物质源特征 ;概述了不同尺度天气系统中上升运动特征 ,沙尘的沉降末速计算、沙漠地表的湍流输送特征和垂直速度资料、沙漠地表的超绝热梯度出现的频率和强度及逆湿现象 ,沙漠和沙地的沙尘粒度、地表性质和沙土含水率对起动风速的影响。     

Rhizosphere: A new frontier for soil biogeochemistry

A range of key biological functions of plant roots such as uptake, respiration and exudation can considerably alter biogeochemical parameters of the soil in the vicinity of the roots, i.e., the rhizosphere: concentrations of nutrients, toxic elements (e.g., aluminium) and pollutants, concentrations of complexing or chelating compounds, pH and redox potential, partial pressures of gases such as O₂ and CO₂, etc. Such parameters can also be directly influenced by the activities of soil microorganisms that are known to be stimulated by root exudation in the rhizosphere. Changes of biogeochemical parameters of the soil solution occurring in the rhizosphere influence a whole range of reactions at the soil solid/soil solution interface. Higher plants thereby play a key role in (i) the weathering of soil minerals, (ii) soil formation processes (pedogenesis) and (iii) the biogeochemistry of elements that are either beneficial or toxic to themselves and other soil biota.