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1.
采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场,各20尾,取全血或肌肉组织,以酚-仿抽提方法提取基因组DNA,利用筛选获得的10对微卫星引物进行PCR扩增,反应产物经8%非变性聚丙烯酰胺凝胶电泳分离、EB显色,用ImageMasterl D Elite(Version 3.01)软件分析电泳结果,并计算了相应的遗传学参数。结果表明,在自然和养殖群体中,10个基因座位的等位基因平均数(α)分别为6.7和6.1,每个基因座位有效等位基因数(αe)分别为1.8—6.8和2.5—6.7,群体平均杂合度(H)分别为0.8120和0.7310;两个群体间的遗传相似性系数、遗传距离和基因分化系数为0.8558、0.1557和0.0558;自然群体内每个座位上的多态信息含量(PIC)为0.59—0.84、个体识别率(DP)为0.54—0.86、非父排除率(PPE)为0.41—0.72,其累积个体识别率和非父排除率均达到0.9999,表明所选座位属中高识别力的遗传标记,可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。  相似文献   
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This study examines the status and exploitation level of 31 northern European stocks targeted by fisheries certified by the Marine Stewardship Council (MSC) as being sustainable and well managed. In the first year of certification, 11 stocks (52% of stocks with available data) were exploited above the maximum sustainable level and four stocks (16% of stocks with available data) were outside of safe biological limits. MSC states that it certifies sub-standard stocks because they will improve once they are in their program. However, after a duration of certification of one to ten years (average four years), no significant changes in fishing pressure or stock size were detected. In the last certified year with available data, seven stocks (44% of stocks with available data) were subject to overfishing and five stocks (21% of stocks with available data) were outside of safe biological limits. Certification should guarantee that fishing quotas are set correctly and are enforced. However, in 11 stocks quotas were set 20–60% above the level that fishers were taking, whereas in three stocks landings exceeded quotas by 30–50%. The study concludes that MSC should change its rules such that overfishing or unsafe stock sizes lead to immediate suspension of certification and that no certification is issued in the first place for a stock that is already in such a situation.  相似文献   
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红鳍笛鲷亲鱼培育及产卵技术研究   总被引:2,自引:0,他引:2  
采用控温、营养强化的方法培育红鳍笛鲷(Lutjanuserythropterus)亲鱼 ,并结合激素诱导使其在1周年内每个月不间断产卵。实验亲鱼454尾 ,培育成活率为96% ,共采卵147150×104粒 ,平均每尾雌鱼年产卵量为541.0×104粒 ,人工催产卵的受精率为40 %~86.2 % ,自然产卵的受精率为68.3%~91.2%。  相似文献   
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According to the 1995 United Nations Fish Stocks Agreement (UNFSA), straddling fish stocks and highly migratory fish stocks are to be managed by Regional Fisheries Management Organisations (RFMOs), consisting of coastal states and relevant Distant Water Fishing Nations (DWFNs). In the North East Atlantic there are several straddling stocks, including herring, mackerel, blue whiting, redfish and numerous deep sea stocks that are exploited both within coastal states’ 200 mile Exclusive Economic Zones and on the high seas. Management of such stocks poses special management problems. In this area, the North East Atlantic Fisheries Commission (NEAFC) represents the relevant RFMO. So far the literature has devoted little attention to RFMOs in general and to NEAFC in particular. The purpose of this report is, first, to provide an overview of the organisation, structure, and objectives of NEAFC and, second, to consider its performance with regard to resource management.  相似文献   
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Two juvenile slender tuna Allothunnus fallai Serventy, 278 mm and 282 mm fork length, were identified from a catch by a purse seiner 23 km off the Moko Hinau Islands, New Zealand, on 23 January 1974. As the slender tuna were observed to school in association with skipjack tuna Katsuwonus pelamis L., it is probable that adults also school, and could be available as a yet unexploited resource to New Zealand fishermen, especially to the south‐east of New Zealand.  相似文献   
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Five plant communities in Lake Rotoiti, North Island, New Zealand (38° 02’ S, 176° 24’ E) are described. In shallow water (0–2 m depth) partly protected from the prevailing westerly winds, some indigenous species form characteristic mounds. From 2 to 6 m depth the exotic macrophyte Lagarosiphon major (Ridley) Moss is the dominant and forms dense beds which appear to have completely replaced any native vegetation. Elodea canadensis Michx., a longer‐established exotic, may form a minor component of this zone, but may become the dominant species in water above and below the Lagarosiphon zone. Lagarosiphon appears to be primarily restricted to silty sand, but on pure silt areas it is replaced by Elodea and/or Nitella hookeri A. Braun. These zonations are probably static rather than successional. On underwater cliff faces and boulder shores a seasonal succession of algae was the major vegetation. Only filamentous cyanophytes grew within 1–2m of geothermal springs in the lake.  相似文献   
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