不同培养条件下多刺裸腹溞的生殖和种群增长参数

于 １９９６年在实验室对多刺探腹溞（Ｍｂｉｎａ　ｍａｃｒｏｃｏｐａ）种群的增长率及相关生态学参数进行了研究．结果表明,３个温度梯度（２４、２８和３２℃）和３个面包酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓ　ｃｅｒｅｖｉｓｉ－ａｅ）密度（０．２５、２ ５和２５ ｘ １０_６ｃｅｌｌ．ｍＬ~（－１）下,以２８℃时内禀增长力（ｒ_ｍ）最高;２８℃时,面包酵母的投喂密度为２５×１０_６ｃｅｌｌ．ｍＬ_（－１）时,其ｒ_ｍ最大．温度２８℃,面包酵母密度为２５×１０_６ｃｅｌｌ．ｍＬ~（－１）时,该溞的繁殖前期最短,繁殖期和寿命均最长,并且怀卵量（每窝产仔数）最大．该溞的种群密度和瞬时增长率分别在接种后第７ｄ和第３－４ｄ达高峰．该搔批量培养时采收时间以第５ｄ为宜．     

利用酒精酵母细胞表面展示技术制备活疫苗及其潜在应用

最近几年,出现把有关的基因在宿主表达之后的目的蛋白分泌并固定在细菌、病毒和酵母菌细胞表面上的技术一表面展示技术。在这些宿主中,酒精酵母菌是最佳的微生物。本文对利用酒精酵母细胞表面展示技术制备活疫苗的基本原理,在酵母细胞表面展示蛋白质的优点,以及哈维氏弧菌溶血素蛋白在酵母菌细胞表面上展示及其作为活疫苗的潜在应用作了综述。研究结果表明在酒精酵母菌细胞表面上展示的哈维氏弧菌溶血素蛋白在海洋动物中具有很好的免疫原性和免疫保护性。     

Equilibrium,Thermodynamic, and Kinetic Studies on Lead (II) Biosorption from Aqueous Solution by Saccharomyces cerevisiae Biomass

This paper presents a biosorption procedure for the preconcentration of Pb²⁺ ions using Saccharomyces cerevisiae biomass. The influence of several factors including pH, biomass dosage, contact time, and temperature on biosorption efficiency were optimized. At optimum value of all the equilibrium, thermodynamic, and kinetic parameters of Pb²⁺ ion biosorption was investigated by testing the Langmuir and Freundlich models and first and second order kinetic models were applied. The biosorption capacity of S. cerevisiae biomass was determined 89.6 mg/g, while the retained Pb²⁺ ions by S. cerevisiae were reversibly eluted using 5 mol/L HNO₃. Due to the high stability of S. cerevisiae the applied biomass can be used successively ten times with a slightly decrease (about 20%) in the recovery of Pb²⁺ ions. The calculated thermodynamic parameters, ΔG°, ΔH°, and ΔS° showed that the biosorption of Pb²⁺ ion onto S. cerevisiae biomass was feasible, spontaneous, and endothermic under examined conditions. The results of kinetic analysis showed that the biosorption processes of Pb²⁺ ions onto S. cerevisiae biomass followed pseudo second order kinetics.     

Utilization of Banana Stem Juice as a Feedstock Material for Bioethanol Production

Bananas are widely cultivated in tropical and subtropical countries and about 220 tons of biomass waste is produced per hectare of banana plantation. Banana pseudostem contains nearly 90% of moisture and about 4–5 m³ sap is generated from one ton of dried stem with high chemical oxygen demand(COD) and biological oxygen demand (BOD). The feasibility of using banana sap as a feedstock to produce ethanol is evaluated in this study. Banana sap is obtained by crushing the pseudostems and concentrated ten times and supplementing with other industrial byproducts such as corn steep liquor(CSL), spent wash (SW), and yeast extract (YE) for ethanol production. Acid and alkali hydrolyzes are performed to enhance the sugar levels of the sap before fermentation. Two different strains of Saccharomyces cerevisiae (MTCC170 and MTCC180) are used for fermentation. In general, supplementation of banana sap with industrial byproducts significantly enhanced the ethanol production. The maximum ethanol production (2.5 g ^?1) is observed with concentrated banana sap supplemented with 25% SW (v/v) with MTCC170, which is 16‐fold higher than banana sap alone. Theethanol content is also higher in alkali‐hydrolyzed banana sap supplemented with 25% SW compared to control. These results suggest that banana sap can be used as a renewable source to produce ethanol by supplementing with other industrial byproducts.