长江口水域双带蛤科一新种

于１９８５－１９８６年在长江口的底栖生物取样中，采到大量双壳类软体动物标本。鉴定表明，其中一新种，被定名为中华团结蛤Ａｂｒａ ｓｉｍｉｃａ ｓｐ．ｎｏｖ。，属樱蛤超科中的双带科（Ｓｅｍｅｌｉｄａｅ）。模式标本保存于中国科学院海洋研究所。     

几种消毒剂对不同发育时期卤虫的影响

研究了几种常用的消毒剂（高锰酸钾、鲁歌氏碘液、新洁尔灭溶液、孔雀石绿）对山西两性卤虫不同发育时期的影响。结果表明：不同发育时期的卤虫对同一试剂有敏感性有明显的不同,其中后无节幼虫是最敏感时期;卡方检验结果表明,卤虫对所用试剂的安全剂量分别是：高锰酸钾≤１ｐｐｍ,鲁歌氏碘液≤１ｐｐｍ,新洁尔灭溶液≤２０ｐｐｍ,孔雀石绿≤０．２ｐｐｍ;由于不同试剂的药理作用不同,因此卤虫对不同试剂的耐受力,并不遵循一     

A new species of the genus Paramisophria (Copepoda, Calanoida,Arietellidae) from the China Seas

ＡｎｅｗｓｐｅｃｉｅｓｏｆｔｈｅｇｅｎｕｓＰａｒａｍｉｓｏｐｈｒｉａ（Ｃｏｐｅｐｏｄａ，Ｃａｌａｎｏｉｄａ，Ａｒｉｅｔｅｌｌｉｄａｅ）ｆｒｏｍｔｈｅＣｈｉｎａＳｅａｓ￥ＬｉａｎＧｕａｎｇｓｈａｎａｎｄＱｉａｎＨｏｎｇｌｉｎ（ＲｅｃｅｉｖｅｄＦｅｂｒｕ...     

中国卤虫早期胚胎发育相关基因的研究——卤虫幼体trh基因的整体荧光原位杂交技术的建立

中国卤虫(Artemia sinica)是重要的饵料生物,是当前海洋生物学研究的热点生物和重要的生物模型。本文报告了卤虫无节幼体整体荧光原位杂交(WFISH,whole-mount fluorescent in situ hybridization)技术,用于快速对卤虫早期发育相关基因的特定时空表达图式进行分析,有助于对卤虫早期发育相关基因功能进行鉴定。人工合成带有FITC标记的卤虫trh基因(trachealess gene)寡核苷酸探针,检测到trh基因在卤虫无节幼体的头部背面盐腺中表达。     

沙地麻黄育苗试验研究

文章阐述了麻黄（ＥｐｈｅｄｒａｓｉｎｉｃａＳｔａｐｆ）的生物学特性、引种繁殖技术及育苗效果,认为覆膜育苗在生产上有较高的推广价值,可以很好地开发麻黄的药用资源。     

The experimental studies on the carbon and nitrogen budgets of Pseudeuphausia sinica

Abstract-The carbon and nitrogen budgets were estimated on the adult females, juveniles and post-furcilia larvae of Pseudeuphausia sinica fed on newly hatching nauplii of Artemia salina in the laborato-ry. It was found that the ingestion rate was linearly related to the food concentration, suggesting highfeeding potential. The linear correlation could be established between the respirating rate (carbon con-sumption rate) and carbon ingestion rate, as well as carbon assimilation rate. The regression coefficients(i.e.specific dynamic action coefficients) were in the range from 9% to 16% (ingested C) or 10% to17% (assimilated C) respectively, with lower in the post-furcilia larvae. There also existed a linearcorrelation equation between estimated total nitrogen excretion rate and the rates of nitrogen ingestionand assimilation separately, except for the juveniles. The defecation rates increased with the increase ofthe ingestion rate; as a result, assimilation efficiency was not related to the ingestion rat     

Results of an Intentional Introduction of Artemia sinica in the High-Altitude Tibetan Lake Dangxiong Co:On a Base of Surveys in 2011 and 2013

正1 Introduction The human population is projected to reach 9200million by 2050.A fundamental question for science is whether it is possible to increase food production to meet the demands of a human population of that magnitude.It’s possible only to increase an aquaculture production.Cultivation of fish/shrimp larvae is a bottleneck in a cultivation of the different organisms.Live food     

Effect of regional warming on the abundance of Pseudeuphausia sinica Wang et Chen (Euphausiacea) off the Changjiang River (Yangtze River) Estuary

Distribution and abundance of Pseudeuphausia sinica off the Changjiang River Estuary (30 ° 00′ - 32 ° 00′ N, 122 ° 00′ -123 ° 30 ′E), the East China Sea were studied in relation to environmental features associated with the regional warming. P. sinica is a subtropical species. Off the Changjiang River Estuary, its abundance reached maximum in summer. To examine spatial and temporal changes of P. sinica off the Changjiang River Estuary, the authors have combined all available sampling data in 1979, 1981, and 2000-2007. This database shows that a significant increase in abundances of P. sinica was observed in spring of 2000-2007 as compared with 1979, 1981. The abundance of P. sinica increased from 0.18-0.21 ind./m 3 in 1979 and 1981 to 0.68-4.00 ind./m 3 in 2000-2007. Accordingly, the sea temperature increased obviously from spring of 1979, 1981 to the 2000s. The authors further found a positive relationship between average surface temperature and average abundance of P. sinica. Regional warming, together with the release of predator induced stress due to a sharp decline in the abundance of its predator (e.g., fishes), were thought to be responsible for the increase in abundance of P. sinica in water off the Changjiang River Estuary.     

Genetic Parameters of Body Length and Response to Selection for Growth Across Four Generations of Artemia sinica

To investigate the genetic components of growth in the brine shrimp Artemia sinica, we estimated the genetic parameters of body length and the response to selection using a fully pedigreed population of A. sinica. The base population was generated from four wild founder populations. We tested 4160 offspring in 360 families over four generations for growth and survival performance. Across four generations, we produced full-and half-sib families with nested mating, where two dams were mated to the same sire. Individual body length was measured for each nauplius at day 20 post-hatching. Heritability of body length was estimated across four generations with the restricted maximum likelihood method. The heritability of body length in A. sinica was low(0.14 ± 0.05), and the common environmental effect was 0.14 ± 0.02. We estimated the response to selection for body length by calculating the difference in the mean breeding values between different generations. The accumulated genetic gain in body length was 278.94 μm after three generations of selection. This low response to selection was probably caused by the low heritability of body length, small sample size, and the low selection intensity(50%). The results suggest that A. sinica selective breeding programs must be changed to generate any substantial, sustainable genetic increases in body length. We suggest that optimal genetic gains could be achieved by introducing wild strains into the nuclear breeding population to increase genetic variation, and by increasing the size of the breeding population to allow for increased selection intensity.     

中国卤虫(Artemia sinica)早期胚胎发育相关基因表达序列标签(ESTs)分析

以mRNA差异显示技术分析了中国两性生殖卤虫早期胚胎发育相关基因的表达序列标签(ESTs),共回收了2000条差异条带并进行了克隆、测序,在GenBank/DDBJ/EMBL数据库中用BLASTX和BLASTN软件分别与NCBI上的非冗余核酸序列数据库进行同源性比对,得到分值较高、长度在100bp—2kb以内的ESTs序列133条。所发现的133条ESTs的生物学功能分类为:能量代谢占24%、细胞生长、分裂相关基因占23%、RNA相关占17%、体节形成占12%、蛋白质合成占3%、转录因子占3%、离子转运占5%、细胞信号转导占4%、未知占9%。对所发现的ESTs进行了RT-PCR测定,排除假阳性能够提供足够数量的信息用于获取其全长cDNA,进而获得其全长基因序列以用于功能研究。另外,研究发现了包括小热休克蛋白基因家族、Hox基因家族的abdA和Dfd基因、A-trh基因(排盐器官及盐调节基因)、HIF(缺氧诱导因子)、CK-M2(肌肉型磷酸激酶基因)和GRP基因(富甘氨酸蛋白基因)等20个具有明显功能的相关基因的ESTs和完整基因序列和开放阅读框(ORF)。经初步推断这些ESTs和基因的功能大多为与卤虫发育的生理、生化相关的基因有关。所研究的133条ESTs基本代表了卤虫早期胚胎发育相关功能基因的表达谱。