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1.
以酪蛋白和明胶为蛋白源 ,添加豆油、鱼油、糊精、纤维素以及适量的维生素和矿物质混合剂配制试验饲料 ,饲料配成5个蛋白质水平 ,每个蛋白水平分别包括5个能量级 ,以体质量增长倍数、蛋白质效率和饲料系数为评判依据 ,对体质量为8.73g±0.79g的中华鲟(Acipensersinensis)的适宜蛋白质含量及其蛋白能量比进行了研究。经过为期8周的试验 ,结果表明 :中华鲟幼鲟配合饲料中的适宜蛋白质含量范围为35.7%~41.8% ,饲料的最佳蛋白能量比范围为21.85~30.05ng/J。当试验饲料蛋白质含量为40.3% ,蛋白能量比为27.85ng/J时 ,试验鲟鱼获得最大体质量增长倍数为11.60±1.20,最低的饲料系数为1.68±0.23,以及最高的蛋白质效率为1.58%±0.14%。  相似文献   
2.
蛋白质组学的研究概况及其在海洋生物学中的应用   总被引:7,自引:0,他引:7       下载免费PDF全文
随着DNA序列测定技术的飞速发展,人们可以在很短的时间内获得某种生物基因组的全序列。生物体的生命活动主要依赖于基因组的产物——蛋白质组,但是蛋白质组的研究相对于基因组来说却要复杂得多。蛋白质组技术是在细胞内整体蛋白质水平上进行研究,从蛋白质整体活动的角度来研究生命活动的规律。综述了蛋白质组和蛋白质组学出现的背景、起源、概念、研究技术、研究内容、面对的挑战、开展该项研究的意义、陆生动植物和微生物蛋白质组学的研究进展及海洋生物蛋白质组学的研究概况。目前,普通生物方面的蛋白质组工作开展得较多,海洋生物方面却相对滞后。种种迹象表明,开展海洋生物蛋白质组学研究,对揭示生命的奥秘、促进海洋资源的开发都有重大的推动作用。  相似文献   
3.
The bioaccessibility of metals (Al, Ca, Fe, Mn, Ag, Cd, Co, Cu, Ni, Pb, Sn, Zn) in oxic estuarine sediments has been evaluated using solutions of a commercially available protein (bovine serum albumin; BSA) that mimic the chemical conditions encountered in the gut environment of many deposit-feeding organisms. Over a 20 h incubation period with 5 g L− 1 BSA, metal mobilisation was generally biphasic in that a relatively short period of rapid release was succeeded by more gradual release or approach to equilibrium, although in some cases metal readsorption was evident during the time-courses. Availability to BSA, defined as metal released after 20 h relative to metal extracted by boiling aqua regia, was greatest for Cd, Ni, Ca and Zn and lowest for Fe and Mn, and correlated well with, but was an order of magnitude lower than, metal digested by acidified hydrogen peroxide. Time-courses conducted in the absence of the protein revealed that significant quantities of Ca and Mn were water-soluble, reflecting the partial dissolution of carbonates and hydrous Mn oxides, respectively. Additional experiments indicated a net increase in metal release with increasing BSA concentration and, with the exception of Ca and Mn, a substantial increase in metal mobilisation after sediment organic matter had been digested by peroxidation. These observations suggest that, apart from Ca and Mn, metal release proceeds via complexation with component amino acids of the protein, denudation of organic host phases, and exposure of inorganic, metal-bearing minerals. Accordingly, the bioaccessibility of a metal is predicted to be dependent on its (i) affinity for proteinaceous ligands, (ii) association with components of digestible sediment organic matter, and (iii) degree of binding at relatively weak sites on sediment phases that are exposed or modified by the action of proteins and other chemical constituents of the gut environment.  相似文献   
4.
益母草水苏碱对钉螺生理生化的影响   总被引:1,自引:1,他引:1  
本实验研究了益母草水苏碱对钉螺糖原和总蛋白的生理生化影响:大于0.80g/L益母草水苏碱能够显著降低钉螺体内的糖原含量,减少幅度从11.68%到76.16%;益母草水苏碱也能够在一定程度上降低钉螺体内的蛋白质含量(从5.66%到13.69%),但减少量不及糖原显著;灭螺植物对钉螺的致死作用可能更多与钉螺组织细胞中的能量代谢有关。  相似文献   
5.
该文用几何光学与辐射传输混合模型研究不连续植被冠层的几何光学反射模型的四分量(承照树冠、承照地面、阴影树冠、阴影地面)的参数化。用一个修正的均匀介质层路径散射(反射与传输)参数的解析算法估计路径散射参数(反射与传输),其中也考虑了冠层间隙的影响。光谱分量特征是不连续植被冠层的传输与反射,背景反照率,以直射光通量与天空漫射光通量比例的函数。光谱分量特征的模型与在美国缅因州Holand采集的针叶林数据吻合。基于LiStrahler几何光学相互遮蔽模型,用参数化的光谱分量特征对老松林和老云杉林的方向反射进行估计,其结果与在不同太阳与观测方向上的PARABOLA测量值匹配得很好。  相似文献   
6.
POLDER(地球反射极化和方向)仪器在BOREAS实验中曾搭载NASA的一架C130飞机飞行。在BOREAS的南部地区,POLDER获取了各种实验场地上的BRDF测量值。森林覆盖地区的大的热点特征,以及十个地区上的镜面反射分量得到了描述。该文通过POLDER的测量数据,对向常规的光谱特征中加入通过遥感获得的方向性特征后,对各种森林覆盖的分类和区分能力的提高给出了定量化的解。当将方向信息加入常规光谱信息后,采用无监督分类时,类间耦合矩阵的各项显著减小(减小倍数为2-5倍)。这一事实证明了用遥感方向特征可以增强对BOREAL森林覆盖的区分能力。  相似文献   
7.
The bimodal NW Etendeka province is located at the continentalend of the Tristan plume trace in coastal Namibia. It comprisesa high-Ti (Khumib type) and three low-Ti basalt (Tafelberg,Kuidas and Esmeralda types) suites, with, at stratigraphicallyhigher level, interstratified high-Ti latites (three units)and quartz latites (five units), and one low-Ti quartz latite.Khumib basalts are enriched in high field strength elementsand light rare earth elements relative to low-Ti types and exhibittrace element affinities with Tristan da Cunha lavas. The unradiogenic206Pb/204Pb ratios of Khumib basalts are distinctive, most plottingto the left of the 132 Ma Geochron, together with elevated 207Pb/204Pbratios, and Sr–Nd isotopic compositions plotting in thelower 143Nd/144Nd part of mantle array (EM1-like). The low-Tibasalts have less coherent trace element patterns and variable,radiogenic initial Sr (  相似文献   
8.
Thanks to its simple division into agricultural and forestry land use, the Corbeira catchment (Galicia, Spain) is used as a case study to build a predictive model using hydrogeochemical signatures. Stream data acquired under recessional flow conditions over a one year period were obtained from a sampling station near the downstream end of the catchment, and using principal component analysis, it is shown that some of the analytical parameters are covariant, and some are negatively correlated. These findings support inferences about the pathways of rainfall in the catchment. Specific signatures may be associated with the dominant hydrological source, either surface runoff or subsurface waters: additionally, the dominant land use in that part of the catchment, where the flow originated, can also be predicted. The dominant runoff shows a strong covariance between suspended solids (SS) and particulate phosphorus (PP), with a clear negative correlation with pH. Dissolved organic carbon (DOC) data are associated with this covariant set when these compounds are available in the soils in question. Dissolved phosphorus, total organic nitrogen and dissolved nitrates are also associated with the same covariant set when the runoff flows through areas of extensive agricultural use. The SS ? PP covariance is less significant at lower flows. Typical base flow regimes show a significant covariance between salinity and pH, with a marked negative correlation with SS ? PP set, confirming the dominance of subsurface waters in the baseflow, as expected. Seasonally divergent DOC ? SS behaviour proves to be a useful tracer for rainfall regimes. The DOC trend shows a sinusoidal annual variation in amplitude, determined by the rainfall regime. As a result, flow from the catchment is dominated by surface water whenever there is synchronicity between the peaks of DOC and SS. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   
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10.
基于目标时域散射特性的土地覆盖类型分类研究   总被引:2,自引:0,他引:2  
邵芸  范湘涛  刘浩 《国土资源遥感》2001,12(4):40-49,67
目标散射特性随时间变化的规律称之为目标时域散射特性。目标时域散射特征是利用多时相雷达遥感图像进行目标识别的基础。本研究以广东肇庆为试验区,利用多时相单参数雷达图像进行土地覆盖类型分类研究,分析了试验区内典型植被的结构、形态及其散射机理等特征,总结了各类目标的时域散射特性,区分识别了多种目标,制作了土地覆盖类型分类图.  相似文献   
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