Effects of Nutritional Factors on the Growth and Heterotrophic Eicosapentaenoic Acid Production of Diatom Nitzschia laevis

The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4 LDM (quadrupled concentration of the nutrient salt) was the optimal concentration of nutrient salt for the growth and EPA production ofN. laevis. The growth ofN. laevis was inhibited when the glucose concentration was either lower than 10gL-1 or higher than 15gL-1. Both sodium nitrate and urea were good nitrogen sources for the growth and EPA production, while ammonium chloride seriously decreased the dry cell weight (DW) and the EPA content. Silicate seriously influenced the growth of N. laevis. The maximum DW of 2.34gL-1 was obtained in the presence of 150 mgL-1 Na2SiO3·9H2O. The EPA content remained almost the same when the silicate concentration was lower than 150 mgL-1; however, higher silicate concentrations resulted in a steady de- crease of EPA content. Low medium salinity (≤29) did not seem to influence the DW of N. laevis, and high salinity resulted in a decrease of DW. The highest EPA content (4.08%) and yield (110 mgL-1) were observed at the salinity of 36 and 29, respectively.     

厦门海域大型底栖动物两个优势种的发现及其数量分析

根据2004年在厦门海域22个取样站获得的4个季度的大型底栖动物调查数据以及历史资料,发现软体动物双壳纲的光滑河蓝蛤(Potamocorbula laevis)是厦门海域大型底栖动物群落的局部、季节性优势种;环节动物多毛纲的昆士兰稚齿虫(Prionospio queenslandica)是局部优势、周年常见种。春季和夏季,光滑河蓝蛤在鼓浪屿附近海域的密度分别为1098.8个/m2和1720.0个/m2,夏季,在曾厝安附近海域的密度为3773.3个/m2,均占所在海域大型底栖动物密度的50%以上,而冬季和秋季,很少采集到光滑河蓝蛤。夏季和秋季,昆士兰稚齿虫在鼓浪屿附近海域的密度分别为871.3个/m2和232.5个/m2;从季度平均值看,昆士兰稚齿虫密度和生物量夏季最高,秋季次之,春季第三,冬季最低;昆士兰稚齿虫的优势度没有光滑河蓝蛤高,但在整个厦门海域均有一定的密度和生物量。本研究分析了光滑河蓝蛤、昆士兰稚齿虫数量与有机质、硫化物含量、水动力等环境因子之间的关系。