钝吻黄盖鲽同工酶组织特异性及群体遗传结构的初步研究

采用水平淀粉凝胶电泳技术对产于山东蓬莱水域的野生钝吻黄盖鲽同工酶组织特异性及其群体遗传结构进行研究。对钝吻黄盖碟的眼、鳃、肌肉、心脏、肝脏、肾脏、脾脏和鳍条共8种组织的15种同工酶分析结果表明,钝吻黄盖碟同工酶的表达呈明显的组织特异性。选择肝脏和肌肉2种组织的AAT,ADH,GPI,G3PDH,IDHP,LDH,MDH,MPI,PGDH,PGM,SDH和SOD共12种同工酶进行了钝吻黄盖碟种群结构遗传学分析,共记录了17个基因座位,其中,PGDH*,LDH*,PGM-1*和MPI-1*等4个基因座位呈多态,其多态座位比例为0.235 3(P0.99),观测杂合度和预期杂合度的平均值分别为0.019 6和0.018 1,平均有效等位基因数为1.093 0。     

Identification of transcriptional effects of ethynyl oestradiol in male plaice (Pleuronectes platessa) by suppression subtractive hybridisation and a nylon macroarray

Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially expressed in response to ethynyl oestradiol (EE2) exposure in liver from male plaice (Pleuronectes platessa) previously analysed for vitellogenin (VTG) induction. Characterisation of the cDNA clones identified many as VTG (2 genes) and zona radiata proteins (ZRP) (3 genes), but 40 encoded other proteins, with more than half cryptic. Further analysis identified 85 non-redundant clones suitable for array on nylon membrane. Radiolabelled cDNAs were prepared from hepatic mRNA from EE2 treated plaice (0 and 21 days) and hybridised with the arrayed clones. Analysis of the data showed that 11/17 novel, 21/22 VTG, 13/14 ZRP, 2/2 liver aspartic proteinase (LAP) and 8/10 other mRNAs were up-regulated by EE2 exposure.