The variation patterns and correlations of total phosphorus, organic phosphorus and phosphate in the Changjiang Estuary

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The effects of immobilization on physiology and ultrastructure of unicellular alga Platymonas subcordiformis

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三苯基氯化锡对扁藻细胞超微结构的影响

于１９９２年４月－１９９２年６月以扁藻为材料进行半抑制深度三苯基氯化锡处理７２ｈ实验，用透射电镜观察观察中毒状态扁藻细胞超微结构的变化。结果表明，线粒体发生水肿，内脊局部瓦解，基质内出现由双层膜包围的同心质膜轮；叶绿体内光合片层的网状结构被破坏，光合片层上下重叠成厚块状；蛋白核中央髓部肿胀，淀粉鞘破裂成多角形淀粉板，并分散在髓部周围；叶绿体基质中出现大量淀粉粒，眼点因受淀粉粒挤压而由藻体中下部移至     

海洋卡盾藻对青岛大扁藻的化感作用及其对UV-B辐射的响应

本研究以海洋微藻——赤潮藻海洋卡盾藻(Chattonella marina)与饵料藻青岛大扁藻(Platymonas helgolandica var.tsingtaoensis)为研究对象,采用单培养和共培养的方法,研究二者间的竞争关系,并在此基础上研究海洋卡盾藻去藻过滤液和藻细胞裂解液对青岛大扁藻的化感作用,同时进一步探讨两种海洋微藻间的化感作用对UV-B辐射增强的响应。结果显示:共培养条件下,海洋卡盾藻在2个高浓度下对青岛大扁藻的生长产生显著抑制(P0.05);海洋卡盾藻去藻过滤液和藻细胞裂解液对青岛大扁藻的生长也具有显著抑制作用(P0.05),且藻细胞裂解液的抑制作用更强,说明海洋卡盾藻对青岛大扁藻产生化感作用,且通过细胞间直接接触传递的化感物质多于通过介质传递的。不同密度比例的2种藻共培养组用UV-B辐射(2.16J/m2)处理后,海洋卡盾藻对青岛大扁藻生长的化感作用有所减弱。     

细基江蓠繁枝变型对水体硝态氮变化的生理响应

通过静态模拟实验,比较研究了6个硝态氮(NO3--N)摩尔浓度水平(0,10,20,40,60,80μmol/L)下培养的细基江蓠繁枝变型(Gracilariatenuistipitatavar.liuiZhangetXia)的丙二醛(MDA)、游离脯氨酸含量以及硝酸还原酶(NR)、超氧化物酶歧化酶(SOD)、过氧化物酶(POD)活性的变化。结果表明,随着水体NO3--N摩尔浓度的逐渐增加,细基江蓠繁枝变型NR、SOD和POD的活性均呈现先增加后下降的显著变化(P<0.05),膜脂过氧化产物MDA含量随水体NO3--N摩尔浓度的变化则呈相反的趋势(P<0.05);游离脯氨酸含量则与水体NO3--N摩尔浓度呈正相关(r=0.9161)。综合分析结果认为,单独以硝态氮为氮源时,20μmol/L的供氮水平能使细基江蓠繁枝变型处于相对最佳的生理状态。     

大型海藻孔石莼抑制浮游微藻生长的原因初探--种群密度及磷浓度的作用

本文以磷浓度和种群密度为变量 ,以亚心形扁藻为浮游微藻代表 ,采用实验生态学手段研究了大型海藻孔石莼与浮游微藻 (亚心形扁藻 )间的竞争。结果显示 ,不论营养盐浓度如何 ,高密度的孔石莼皆能显著抑制浮游微藻的生长。分析显示 ,高密度的孔石莼压制浮游微藻生长的原因 ,不是由于对氮、磷等营养盐的竞争所致 ,而可能是其他原因所致。     

Study on the Free Radical Scavenging Activity of Sea Cucumber (Paracaudina chinens var.) Gelatin Hydrolysate

Gelatin from the sea cucumber(Paracaudina chinens var.) was hydrolyzed by bromelain and the hydrolysate was found to have a high free radical scavenging activity. The hydrolysate was fractionated through an ultrafiltration membrane with 5 kDa molecular weight cutoff(MWCO). The portion(less than 5 kDa) was further separated by Sephadex G-25. The active peak was col-lected and assayed for free radical scavenging activity. The scavenging rates for superoxide anion radicals(O2·-) and hydroxyl radi-cals(·OH) of the fraction with the highest activity were 29.02% and 75.41%,respectively. A rabbit liver mitochondrial free radical damage model was adopted to study the free radical scavenging activity of the fraction. The results showed that the sea cucumber gelatin hydrolysate can prevent the damage of rabbit liver and mitochondria.     

洞庭青鲫(Carassius auratus var.Dongtingking)与三个鲫品系群体遗传多样性的ISSR分析

采用ISSR分子标记技术,对洞庭青鲫(Carassius auratus var.Dongtingking)、野生二倍体和三倍体鲫(C.auratus)、彭泽鲫(C.auratus var.Pengze)4个鲫品系群体共120个个体的遗传多样性进行分析。9个ISSR引物在四个鲫品系群体中共获得1637个扩增位点,其中多态位点1572个,多态位点比例为96.03%。4个鲫品系群体内的多态位点比例分别为46.14%、65.40%、73.76%和51.94%,遗传距离分别为0.0905、0.1186、0.1351和0.1056。在4个鲫品系群体之间,洞庭青鲫与彭泽鲫的遗传距离最小(0.1191),二倍体鲫和三倍体鲫的遗传距离次之(0.1336),而洞庭青鲫、彭泽鲫与二倍体鲫的遗传距离(分别为0.1377、0.1367)略小于它们与三倍体鲫的遗传距离(均为0.1378)。UPGMA聚类结果为洞庭青鲫与彭泽鲫聚成一个分支,二倍体鲫与三倍体鲫聚成另一个分支。结果表明,洞庭青鲫、彭泽鲫等养殖群体内的遗传多样性明显低于野生鲫群体;四个鲫品系中,洞庭青鲫与彭泽鲫的亲缘关系较近,二倍体鲫与三倍体鲫的亲缘关系较近,推测洞庭青鲫、彭泽鲫、三倍体鲫均起源于二倍体鲫。二倍体洞庭青鲫和二倍体野生鲫资源的保护必须引起重视。     

A tree-ring based precipitation reconstruction for the Mohe region in the northern Greater Higgnan Mountains,China, since AD 1724

August–July precipitation has been reconstructed back to AD 1724 for the Mohe region in the northern Greater Higgnan Mountains, China, using Pinus sylvestris var. mongolica tree-ring width. The reconstruction explains 39% of the variance in the precipitation observed from AD 1960–2008. Some droughts noted in historical documents are precisely captured in our reconstruction. Wet periods occurred during the periods of AD 1734–1785, AD 1805–1830, AD 1863–1880, AD 1922–1961, and AD 1983–1998; while the periods of AD 1786–1804, AD 1831–1862, AD 1881–1921, and AD 1962–1982 were relatively dry. Power spectral and wavelet analyses demonstrated the existence of significant 24-yr, 12-yr, and 2-yr cycles of variability. The results of the spatial correlations suggest that our reconstruction contains climatic signals for the southern Stanovoy Range and the northern Greater Higgnan Mountains. The positive correlations between the new reconstructed precipitation series and two precipitation reconstructions indicate that our precipitation reconstruction captures broad-scale regional climatic variations. A comparison between the weakening tendency of summer monsoon and the dry period of our reconstruction reveals that the annual precipitation in the Mohe region is partly influenced by the East Asian Summer Monsoon.     

青藏高原东北部观赏地被植物马蔺根系固土护坡效应研究

为了进一步丰富青藏高原东北部地区植被护坡工程植物选择,推动区内植被护坡工程实用性和观赏性兼顾发展。该项研究以青藏高原东北部观赏地被植物马蔺为研究对象,以青海省大通县朔北藏族乡至东峡镇之间区域为研究区,在野外植物分布特征和生长特征调查的基础上,结合单根拉伸试验和重塑根-土复合体三轴压缩试验,定量评价了野生马蔺植株和根系生长特征、单根抗拉特性以及根系对土体抗剪强度的增强效应。试验结果表明：马蔺主要分布在研究区内洪积扇、坡积裙和河漫滩等地貌单元,立地条件包括上述地貌单元内的林下坡地、田间、道旁、湿地、荒地等;通过选取两处试验区开展调查可知,马蔺平均株高、冠幅和植株密度分别为75.22±11.40 cm、106.09±25.62 cm和1.51±0.55 株/m2;马蔺标准株根系分布深度可达50 cm,主要分布在0~30 cm深度范围内,根幅约40~50 cm;马蔺根径为0.20～0.70 mm,单根抗拉力、抗拉强度和延伸率平均值分别为7.94±2.91 N,46.30± 11.06 MPa和54.17%±17.08%,随着根径的增大,单根抗拉力和单根抗拉强度分别呈幂函数增大和幂函数降低关系,单根极限延伸率随着根径的增大呈逐渐增大变化趋势,但二者之间未表现出相对显著性的关系;马蔺根系对土体黏聚力和内摩擦角均有增强作用,可分别显著增强0~30 cm和0~10 cm深度范围内土体黏聚力和内摩擦角,且对土体黏聚力的增强作用较为显著。该项研究成果对于选用观赏地被植物马蔺进行研究区坡面水土流失、浅层滑坡等地质灾害现象的生态防护工作,具有理论研究价值和实际指导意义。