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花尾胡椒鲷养殖群体遗传多样性的等位酶研究 总被引:5,自引:1,他引:5
应用聚丙烯酰胺凝胶电泳(PAGE)技术对取自厦门火烧屿海水网箱的花尾胡椒鲷人工鱼养殖群体的遗传多样性进行等位酶分析。结果表明:所检测的16种同工酶的24个基因座位中,在XDH和GCDH基因座位上发现多态性,其平均多态位点比例为8.33%,基因座位有效等位基因的平均数Ne为1.083,平均杂合度的观测值Ho为0.00556,预期值He为0.00537,Hardy-Weinberg遗传偏离指数(D)为0.035。与其他鱼类相比较说明了其遗传多样性处于较低水平,文中对此进行了探讨并提出相应的种质资源管理保护措施。 相似文献
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Antarctic ice microalgae Chlamydomonas sp. ICE-L can survive and thrive in Antarctic sea ice. In this study, Chlamydomonas sp. ICE-L could survive at the salinity of 132‰ NaCl. SDS-PAGE showed that the density of 2 bands (26 and 36 kD) decreased obviously at the salinity of 99‰ NaCl compared to at the salinity of 33‰ NaCl. The soluble proteins in Chlamydomonas sp. ICE-L grown under salinity of 33‰ and 99% NaCl were compared by 2-D gel electrophoresis. After shocking with high salinity, 8 protein spots were found to disappear, and the density of 28 protein spots decreased. In addition, 19 protein spots were enhanced or induced, including one new peptide (51 kD). The changes of proteins might be correlated with the resistance for Chlamydomonas sp. ICE-L to high salinity. 相似文献
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斜带髭鲷养殖群体遗传多样性的同工酶研究 总被引:7,自引:0,他引:7
应用聚丙烯酰胺凝胶电泳(PAGE)技术,对取自广东饶平海水网箱养殖的斜带髭鲷(Hapalogenys nitens)人工苗养殖群体同工酶的多态性进行分析。结果表明:在对48尾鱼所检测出的11种同工酶的23个基因座位中,超氧化物歧化酶(SOD)、苹果酸脱氢酶(MDH)过氧化物酶(PER)等酶的基因座位上发现多态性,其平均多态位点比例(P)为17.39%,基因座位有效等位基因的平均数(Ne)为1.174,平均杂合度的观测值(Ho)为0.051,预期值(He)为0.039,Hardy-Weinberg遗传偏离指数(D)为0.291。这说明该养殖群体具有较高的遗传变异水平。 相似文献
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