从商务日语课程设置谈高职教育课程改革

本科院校办高职学院是我国 2 0世纪末出现的一个新生事物 ,如何设置专业课程 ,提高教学质量 ,使学生一出校门就能容纳到直接为经济服务当中去 ,结合商务日语专业的具体情况对这个问题进行了思考。     

Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus

Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.     

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析

采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场，各20尾，取全血或肌肉组织，以酚-仿抽提方法提取基因组DNA，利用筛选获得的10对微卫星引物进行PCR扩增，反应产物经8％非变性聚丙烯酰胺凝胶电泳分离、EB显色，用ImageMasterl D Elite(Version 3．01)软件分析电泳结果，并计算了相应的遗传学参数。结果表明，在自然和养殖群体中，10个基因座位的等位基因平均数(α)分别为6．7和6．1，每个基因座位有效等位基因数(αe)分别为1．8—6．8和2．5—6．7，群体平均杂合度(H)分别为0．8120和0．7310；两个群体间的遗传相似性系数、遗传距离和基因分化系数为0．8558、0．1557和0．0558；自然群体内每个座位上的多态信息含量(PIC)为0．59—0．84、个体识别率(DP)为0．54—0．86、非父排除率(PPE)为0．41—0．72，其累积个体识别率和非父排除率均达到0．9999，表明所选座位属中高识别力的遗传标记，可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。     

中国对虾育苗池和养成池微型污着生物群落的研究

于１９９２年４－１０月在山东省青岛市黄岛前湾育苗场和胶南市红石崖镇邵家村养殖场，采用载玻片挂片和对中国对虾现场取样的方法，研究中国对虾育苗池和养成池载玻片挂 表面和对体表微型污着生物群落的组成和发展。     

中国对虾幼体期视觉器官的发育

于１９９４年３－５月，以中国对虾无节幼体６个亚期，蚤状幼体３个亚期，糖虾幼体３个亚期及仔虾各时期的幼体为材料，用日产Ｏｌｙｍｐｕｓ万能研究显微镜观察研究各期幼体单眼及复眼的发育。结果表明：在中国对虾无节幼体Ｎ１－Ｎ６６个亚期，单眼的两侧均有复眼的原基组织。     

牙鲆种群动态及其资源管理

本文根据1980—1986年间鱼类生物学参数测定,结合体长分布资料的模拟分析,研究黄、渤海区牙鲆种群年龄结构、死亡动态以及在不同年龄和死亡率条件下世代资源生物量变化的规律。模型分析结果表明,当F=1.0和t_c=6.0a时,单位补充量最大群体产量为1492g。由此认为,现阶段牙鲆资源利用的最佳策略是通过增殖放流,增加资源补充;同时应适当控制捕捞压力,减少渔具对幼鱼资源的破坏并增设繁殖保护区,以追求最大持续产量。     

养殖牙鲆细菌性病原分离与鉴定

牙鲆(Paralichthys olivaceus)是我国北方沿海地区的重要经济养殖品种，目前已形成工厂化生产规模。随着养殖密度的增加以及海水污染程度的日益严重，养殖牙鲆出现了各种疾病(曲凌云等，2000； Mo Zhaolan et al.，2001).造成了巨大的经济损失。 1999~2000年，山东省荣成、威海、青岛胶南及黄岛地区养殖牙鲆大面积发病，疾病发生时间多在每年的6~11月.病鱼多为3~10月龄。本文作者对以上牙鲆发病地区进行了临床病症的调查，并进行了病原的分离和初步鉴定。     

用臭氧处理海水对鱼虾的急性毒性效应研究

本实验检测了不同浓度臭氧残留对中国对虾(Penaeus chinensis)、牙鲆（Paralichthys oilvaceus）存活率的影响。结果表明，对虾的耐受力要比牙鲆鱼强，中国对虾在臭氧浓度≥1.0mg/L时，可存活至少48h，而芽鲆在此浓度下3h后，即出现鳃部充血肿胀、呼吸频率加快等不适应现象，48h LC50为0.13mg/L。如果能正确控制臭氧的残留量，利用臭氧净化养殖用水是一个不容质疑的水质净化的好方法。     

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。