全文获取类型
收费全文 | 474篇 |
免费 | 90篇 |
国内免费 | 197篇 |
专业分类
测绘学 | 26篇 |
大气科学 | 108篇 |
地球物理 | 84篇 |
地质学 | 181篇 |
海洋学 | 250篇 |
天文学 | 3篇 |
综合类 | 78篇 |
自然地理 | 31篇 |
出版年
2024年 | 8篇 |
2023年 | 13篇 |
2022年 | 15篇 |
2021年 | 14篇 |
2020年 | 23篇 |
2019年 | 28篇 |
2018年 | 23篇 |
2017年 | 25篇 |
2016年 | 28篇 |
2015年 | 18篇 |
2014年 | 32篇 |
2013年 | 31篇 |
2012年 | 34篇 |
2011年 | 28篇 |
2010年 | 36篇 |
2009年 | 34篇 |
2008年 | 33篇 |
2007年 | 29篇 |
2006年 | 40篇 |
2005年 | 30篇 |
2004年 | 27篇 |
2003年 | 25篇 |
2002年 | 30篇 |
2001年 | 20篇 |
2000年 | 27篇 |
1999年 | 21篇 |
1998年 | 14篇 |
1997年 | 22篇 |
1996年 | 5篇 |
1995年 | 5篇 |
1994年 | 7篇 |
1993年 | 10篇 |
1992年 | 5篇 |
1991年 | 5篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1983年 | 2篇 |
排序方式: 共有761条查询结果,搜索用时 15 毫秒
1.
ImODUcrroNTheVertchrateepidennisisinintirnatecontaCtwiththeenvironmentandre~environmentaladaptions.TheepidermisofterreStrialanbolsiskeratinedwhilethatofamPhibiousandaquaticfonnsisrelataltomucus-produCti0n.Theepidennisadsasafi-nalbatherbetweentheorganismanditsenvironmentandoneofitSrnainfunCtionsistoproteCttheorganismagainstextemalpathogens.lnadditiontoIneCanicalproedonofthebody,theepidertnisofamPhibiansandfishescansededinsandotherantibacterialpeptidesthathelpinthebonedefenSeoftheanhal(Su… 相似文献
2.
Gert Finger Reinhold J. Dorn Alan W. Hoffman Hamid Mehrgan Manfred Meyer Alan F. M. Moorwood Joerg Stegmeier 《Experimental Astronomy》2002,14(2):117-127
Three different methods are presented to subtract thermal drifts and low-frequency noise from the signal of infrared array.
The first is dead pixels with open Indium bumps, the second is reference output as implemented on the Hawaii2 multiplexer,
and the third is dark pixels to emulate reference cells having a capacity connected to the gate of the unit cell field-effect
transistor (FET). The third method is the most effective and yields a reduction in readout noise from15.4–9.4 erms. A novel
method will be described to extend this readout technique to the Aladdin 1 K × 1 K InSb array.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
3.
Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro. 相似文献
4.
中国对虾血细胞酶细胞化学的初步研究 总被引:1,自引:0,他引:1
运用电镜酶细胞化学技术 ,对中国对虾 (Penaeus chinensis)的血细胞和血清中的酚氧化酶、酸性磷酸酶、碱性磷酸酶和过氧化物酶的酶活性部位进行标记。实验结果显示 :酚氧化酶主要以酶原形式存在于大颗粒细胞的颗粒体中 ,酸性磷酸酶和碱性磷酸酶以酶原形式存在于小颗粒细胞的颗粒体中。健康虾和患病虾血细胞中酶活性的表现形式不尽相同 ,在病理情况下 ,颗粒细胞能以细胞解体的方式 ,排出细胞内的颗粒 ,转化成有活性的酶 ,以此提高血淋巴中这几种酶的活性。透明细胞可以吞噬排放颗粒后的颗粒细胞 ,透明细胞中由高尔基体合成的酸性磷酸酶等溶酶体酶主要用于透明细胞的细胞内消化作用。研究认为 :3种血细胞因含有不同的免疫酶以及酶的作用方式不同而具有不同的免疫功能 相似文献
5.
目的:基于生物信息学探讨骨质疏松症与 2 型糖尿病的关系。方法:通过基因表达汇编(GEO)数据库检索骨质疏松症和2型糖尿病的相关数据集,并筛选骨质疏松症-2型糖尿病共同差异表达基因,将数据导入STRING数据库找寻关键基因,以一个单细胞测序数据集作为检验集验证关键基因细胞层面的表达水平,对筛选结果进行基因本体(GO)功能及京都基因与基因组百科全书(KEGG)通路富集分析,并对关键靶点、相关中药进行频数统计及功效分类。结果:骨质疏松症与 2 型糖尿病相关通路集中在 ErbB信号通路、丝活化蛋白激酶(MAPK)信号通路、神经营养素信号通路、NOD样受体信号通路。频数统计结果显示,与骨质疏松症-2型糖尿病关键基因相关的中药有51味,以功效分类,可分为清热解毒药、止痛药、祛风药、补虚药、止呕药。结论:骨质疏松症和2型糖尿病都是复杂的代谢性疾病,涉及众多基因表达网络和信号通路。尽管两种疾病存在复杂的基因网络背景,但是分析结果表明两种疾病之间仍存在高度重合的差异基因表达和信号通路。这表明两种疾病的分子机制密切相关,可能存在共同调控的靶点,因此这些靶点可能成为药物同时干预两种疾病的治疗靶点。 相似文献
6.
顶空气相色谱法测定海水二甲基硫和浮游植物细胞二甲基硫丙酸的研究 总被引:12,自引:2,他引:12
建立了顶空GC/FPD测定海水中二甲基硫(DMS)和浮游植物细胞中二甲基硫丙酸(DMSP)的方法,并研究盐度、温度、气液相比DMS诸因素对DMS顶空灵敏度的影响。该法对DMS测定的相对标准偏差均小于6%,平均回收率为106%,最低检出限为20ng/L。细胞DMSP先经碱作用转化为DMS,在50℃下作用时间不少于6h,峰高与浓度的双对数线性相关系数大于0.99。对1994年冬、1995年夏采自胶州湾 相似文献
7.
本文报道了文昌鱼哈氏窝匀浆刺激蟾蜍足细胞释放精子过程所发生的超微结构变化,进一步证实这种匀浆含有类似脊椎动物促黄体生成素活性物质。 相似文献
8.
把鹧鸪菜藻体切成大小不同的切段、以及任意切碎,培养于添加氮、磷素的消毒海水中,后者分10℃、15℃、20℃、25℃四种不同温度条件培养,结果全部切段、切碎片都很快再生出假根附着于基质,并再生出一至多个叶状体;叶状体长大后从基部长出新的假根成为完整的幼苗(再生苗)。试验表明,大的切段其再生苗生长快、长度大,但出苗率(一定长度的藻体长出再生苗数量)低。培养温度较高时(20—25℃),藻体任意切碎片再生速度快、附着速度快、数量多且较牢固。利用藻体切碎片来培养鹧鸪菜幼苗是可行的;培养的适宜温度为20℃以上、海水比重为1.010左右。本文还对鹧鸪菜损伤藻体再生的情况(繁殖生殖学)进行一些观察和描述。 相似文献
9.
10.
Akira Shibata Kouichi Ohwada Masakazu Tsuchiya Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):91-97
Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate
organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted
for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present
study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater
cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size,
0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and
correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG
to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m,
which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell−1 obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29)
and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest
that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas
this fraction was reduced in the middle layer. 相似文献