从商务日语课程设置谈高职教育课程改革

本科院校办高职学院是我国 2 0世纪末出现的一个新生事物 ,如何设置专业课程 ,提高教学质量 ,使学生一出校门就能容纳到直接为经济服务当中去 ,结合商务日语专业的具体情况对这个问题进行了思考。     

Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus

Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.     

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

温度、体重和摄食水平对花尾胡椒鲷幼鱼粪便比能值及吸收率的影响

鱼类没有消化的食物通过粪便而排出体外.没有消化的食物、黏液、消化道脱落细胞、代谢酶以及细菌等就组成了鱼类的粪便.排粪后所保留的食物能可以通过吸收率来估算,其计算式为:A=C-FC/C×100%,式中A(吸收率)以百分数表示;C(摄食量)等于摄入的食物质量乘以食物比能值;F(粪便排出量)等于排出的粪便质量乘以粪便比能值.吸收率在营养学上又称为消化率.粪便比能值的测定在计算吸收率时是非常必要的,但在以往对鱼类吸收率的研究[1～6]中,由于粪便收集的困难及粪便的样品量通常很少,均没有对不同条件下鱼类所排出粪便的比能值进行单独的测定.     

Embryonic and larval development of Babylonia formosae habei (Altena and Gittenberger, 1981) (Gastropoda:Buccinidae) on China''''s coast

The development of embryos and larvae of Babylonia formosae habei living along the southeast coast of China is observed under laboratory conditions. The egg masses are laid by females on hard substrate at night and each capsule contains 100-500 eggs. Each egg is 250-280 mm in diameter. The first two cleavages of the embryo are meridional and equal, and a polar lobe is produced. Larval kidney, which only consists of a single cell, appears during the gastrula stage on each side of the embryo. The right tentacle develops prior to the left one. At 25-27℃, an intracapsulate veliger stage is reached about 4.5 d after deposition. The larvae hatch on the fifth day as swimming veligers with a shell length of 360 -500 mm. The newly hatched larva can ingest suspended algal cells from the water column and remains in the pelagic stage for 8-10 d. The newly settled juveniles are 900-1 200 mm in shell length.     

Embryonic and larval development of Babylonia formosae habei(Altena and Gittenberger, 1981)(Gastropoda: Buccinidae) on China''s coast

The development of embryos and larvae of Babyloniaformosae habei living along the southeast coast of China is observed under laboratory conditions. The egg masses are laid by females on hard substrate at night and each capsule contains 100-500 eggs. Each egg is 250-280 mm in diameter. The fast two cleavages of the embryo are meridional and equal, and a polar lobe is produced. Larval kidney, which only consists of a single cell, appears during the gastrula stage on each side of the embryo. The right tentacle develops prior to the left one. At 25-27 ℃, an intracapsulate veliger stage is reached about 4.5 d after deposition. The larvae hatch on the fifth day as swimming veligers with a shell length of 360 -500 mm. The newly hatched larva can ingest suspended algal cells from the water column and remains in the pelagic stage for 8-10 d. The newly settled juveniles are 900-1 200 mm in shell length.     

热带海洋珍珠贝类立克次体(RLO)病研究 Ⅷ.养殖大珠母贝贝苗养成期群体RLO病流行病学研究

大珠母贝人工养殖过程中,幼贝死亡是一个严重的问题。1993年11月至1995年5月,采用现场调查、实验室研究和数理分析相结合的方法,在海南省临高县新盈珍珠贝养殖场自然海区吊养的A,B,C3批养殖贝和陵水新村珍珠贝养殖场养殖池吊养的D批养殖贝的发病和死亡情况进行了流行病学凋查.结果显示,大珠母贝幼贝群体的死亡是以暴发性方式发生,幼贝群体的大批量死亡高峰一般发生在4～6月龄期,8个月以后随着龄期的延长,死亡率显著降低.幼贝群体死亡率与贝体平均体长的关系是4cm以下的贝体死亡率较高,1～3cm阶段处于死亡高峰期内,5cm以上的贝体死亡率显著降低.大珠母贝幼贝群体的死亡率与类立克次体感染(即RLO平均感染严重度指数SI)之间存在显著的相关性,在RLO感染的高峰之后或当中部相应伴随贝群体死亡率的高峰,随着RLO感染的降低,贝群体的死亡率也明显降低。在4批养殖贝群体中,仅在A批养殖贝群体样本中发现有少量纤毛虫寄生(感染率为87.5%,感染强度为3.56个/10倍物镜).这几批幼贝群体的死亡与海水温度、盐度之间均无相关性。     

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

Locomotor activity rhythm in the Japanese eel Anguilla japonica elvers

Under artificial LD cycles(6,12,18 L),the elvers of Japanese eel,Anguilla japonica,showed a 24 h cycle of locomotor activity rhythm being most active at light transitions:the eels' activity rose to a primary peak after lights-off,followed by a quiescent period during which they buried into the shelters or lying motionlessly on sand for most of the time,and then reached a secondary peak before lights-on.Elvers could resynchronize their activity rhythm with a new photo cycle within 4 d.Moreover,their activity level at dark phase significantly increased as the light period was prolonged:higher activity levels during shorter dark period.However,the elvers did not display clearly the existence of a circadian rhythm under constant light or dark conditions.The timing of daily activity rhythm evidenced in the Japanese eels may occur through the action of the LD cycles with a weak participation of an endogenous circadian system.In all the LD cycles,over 99% of the activity occurred in the dark phase,indicating that the eels were always nocturnally active no matter what time of day it might be.Under 12 L conditions,the eels' activity level and the time outside sand were significantly elevated both at light and dark phases as temperature increased from 10~15 to 20~25 ℃.The activity rhythm pattern(i.e.,two peaks occurring around light transitions) did not apparently change among temperatures.However,in contrast with the primary activity peaks immediately after lights-off at 20 and 25 ℃,the timing of the primary peaks at 10 and 15 ℃ showed a latency of a few hours following lights-off,indicating the inhibiting effect of low temperature on the eels' activity.