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1.
蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析 总被引:4,自引:0,他引:4
利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌(Coelomactra antiquata)、中国蛤蜊(Mactra chinensis)和四角蛤蜊(Mactra veneriformis)3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列.测序后用DNAstar软件分析了核苷酸差异。结果显示:三种贝类16SrRNA基因片段长度相同,均为306bp(去除引物).核苷酸存在多态性。共有45个变异位点,54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88.9%.与四角蛤蜊的同源性为88.6%.中国蛤蜊与四角蛤蜊的同源性为90.6%。三种蛤蜊ITS2序列分别为390bp(西施舌)、441bp(四角蛤蜊)和466bp(中国蛤蜊)。存在长度多态性.ITS2核苷酸差异分析结果显示.西施舌与中国蛤蜊的同源性为70.9%-71.1%,西施舌与四角蛤蜊的为70.5%-71.0%。中国蛤蜊与四角蛤蜊的同源性为88.1%-88.8%。ITS2序列分析结果与16SrRNA基因片段分析结果一致.2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。 相似文献
2.
We present the software program THERIA_G, which allows for numerical simulation of garnet growth in a given volume of rock
along any pressure–temperature–time (P–T–t) path. THERIA_G assumes thermodynamic equilibrium between the garnet rim and the rock matrix during growth and accounts for
component fractionation associated with garnet formation as well as for intracrystalline diffusion within garnet. In addition,
THERIA_G keeps track of changes in the equilibrium phase relations, which occur during garnet growth along the specified P–T–t trajectory. This is accomplished by the combination of two major modules: a Gibbs free energy minimization routine is used
to calculate equilibrium phase relations including the volume and composition of successive garnet growth increments as P and T and the effective bulk rock composition change. With the second module intragranular multi-component diffusion is modelled
for spherical garnet geometry. THERIA_G allows to simulate the formation of an entire garnet population, the nucleation and
growth history of which is specified via the garnet crystal size frequency distribution. Garnet growth simulations with THERIA_G
produce compositional profiles for the garnet porphyroblasts of each size class of a population and full information on equilibrium
phase assemblages for any point along the specified P–T–t trajectory. The results of garnet growth simulation can be used to infer the P–T–t path of metamorphism from the chemical zoning of garnet porphyroblasts. With a hypothetical example of garnet growth in a
pelitic rock we demonstrate that it is essential for the interpretation of the chemical zoning of garnet to account for the
combined effects of the thermodynamic conditions of garnet growth, the nucleation history and intracrystalline diffusion.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
相似文献
F. GaidiesEmail: |
3.
分析了普通番茄(Lycopersicumesculentum)在NaCl胁迫下酯酶(EST)和过氧化物酶(PRX)同工酶的变化,考察了盐胁迫对10个同工酶位点21个等位基因在特定组织中表达的影响。发现许多EST和PRX等位基因在盐胁迫下表达,产生盐诱导同工酶;另有些等位基因的表达受盐抑制,它们在盐胁迫下的表达活性显著减弱或消失。实验表明,EST和PRX同工酶表达的变化与番茄对盐胁迫的遗传适应性有密切关系。 相似文献
4.
G.T. Ruggerone J.L. Nielsen J. Bumgarner 《Deep Sea Research Part II: Topical Studies in Oceanography》2007,54(23-26):2776
We tested the hypothesis that increased growth of salmon during early marine life contributed to greater survival and abundance of salmon following the 1976/1977 climate regime shift and that this, in turn, led to density-dependent reductions in growth during late marine stages. Annual measurements of Bristol Bay (Bering Sea) and Chignik (Gulf of Alaska) sockeye salmon scale growth from 1955 to 2002 were used as indices of body growth. During the first and second years at sea, growth of both stocks tended to be higher after the 1976–1977 climate shift, whereas growth during the third year and homeward migration was often below average. Multiple regression models indicated that return per spawner of Bristol Bay sockeye salmon and adult abundance of western and central Alaska sockeye salmon were positively correlated with growth during the first 2 years at sea and negatively correlated with growth during later life stages. After accounting for competition between Bristol Bay sockeye and Asian pink salmon, age-specific adult length of Bristol Bay salmon increased after the 1976–1977 regime shift, then decreased after the 1989 climate shift. Late marine growth and age-specific adult length of Bristol Bay salmon was exceptionally low after 1989, possibly reducing their reproductive potential. These findings support the hypothesis that greater marine growth during the first 2 years at sea contributed to greater salmon survival and abundance, which in turn led to density-dependent growth during later life stages when size-related mortality was likely lower. Our findings provide new evidence supporting the importance of bottom-up control in marine ecosystems and highlight the complex dynamics of species interactions that continually change as salmon grow and mature in the ocean. 相似文献
5.
6.
摄食水平和性别对稀有鮈鲫生长和能量收支的影响 总被引:7,自引:0,他引:7
于1995年10月采集本所自繁有ju鲫(约1.03g/ind),在30℃水温下进行摄食-生长实验,研究了从饥饿到饱食5个摄食水平对不同性别稀有ju鲫的生长和能量收支的影响,结果表明,鱼体干物和能量含量不受摄食率和性别的影响,随着摄食率的增加,雌鱼的湿重,干重和能量特定生长率均呈双对数增加,雄鱼湿重特定生长率呈双对数增加,而干物质和能量特定生长率呈指增加,雄鱼生长速度及摄食率低于雌鱼,随着摄食率的增加,食物能损失于粪便中的比例均呈上升趋势,损失于排泄物中的比例变化不大,呈下降趋势,用于代谢的比例呈下降趋势,用于生长的比例呈上升趋势,除饥饿组外,在各个摄食水平,雌鱼食物能用于代谢的比例较雄鱼低,而用于生长的比例较雄鱼高。 相似文献
7.
Lina Li Jean Guenzennec Peter Nichols Pierre Henry Miki Yanagibayashi Chiaki Kato 《Journal of Oceanography》1999,55(5):635-642
Dense populations of bivalves, primarily Calyptogena sp., were observed at cold seeps of the Nankai Trough. Bacterial input to the sediment was estimated through determination of phospholipid ester-linked fatty acid (PLFA) and DNA profiles. Results indicated a bacterial biomass of 109 cells (g dry wt)-1 while individual fatty acid profiles revealed a predominance of monounsaturated fatty acids, mainly 18:1 isomers. The presence of these fatty acids can be interpreted to reflect a response to low temperature and a predominance of psychrophilic bacteria. DNA fragments encoding bacterial ribosomal RNA small-subunit sequences (16S rDNA) were amplified by the polymerase chain reaction method using DNA extracted directly from the sediment samples. From the sequencing results, at least 19 kinds of bacterial 16S rDNAs related to mostly the Proteobacteria and a few gram-positive bacteria were identified. These results suggest that the bacterial community in the Nankai Trough sediments consists of mainly bacteria belonging to the Proteobacteria , , and subdivisions. Bacteria belonging to the and subdivisions, which are known to include epibiont and sulfate reducing bacteria, respectively, were mostly detected in the sediment obtained from inside the area of the Calyptogena community, and the -Proteobacteria may function to supply reduced sulfur to bacterial endosymbionts of Calyptogena. 相似文献
8.
本文报导了采自舟山沿海单齿螺种群的年龄结构、生物量与生长。自然种群的单齿螺年龄组成以1^+和2^+龄占优势,壳高、壳宽、壳口高、厣径、体重等生长参数的回归关系良好,单齿螺的生长完全可以用壳高来表示。单齿螺以第一、二年生长最快。采捕季节可定在四、五月间,采捕规格应在壳高16mm以上。 相似文献
9.
鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响 总被引:2,自引:5,他引:2
采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法,进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明,该菌株用固体培养基培养至24h左右,可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验,用SAS统计软件分析数据,得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上,添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明,胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌;NH4Cl与酪蛋白水解物可抑制蛋白酶的产生;牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用;培养基中蛋白胨浓度为4%时菌体量与ECP蛋白含量达最高值,在蛋白胨浓度为2%时蛋白酶分泌量已稳定;1%的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量,却抑制了蛋白酶的产生。 相似文献
10.
锶诱导的氧化胁迫对叉鞭金藻(Dicrateria inornata)的影响 总被引:3,自引:2,他引:3
采用不同浓度的锶胁迫方法研究了叉鞭金藻生长、叶绿素、MDA含量及三种抗氧化酶SOD、CAT、GPX活性的变化。结果表明,在实验设计的各种Sr^2 浓度内叉鞭金藻均能生长,但Sr^2 浓度较高时生长受到不同程度的抑制,细胞数量比对照组分别减少15.9%和51.0%;叉鞭金藻中叶绿素含量随着Sr^2 浓度的升高而降低,呈负相关关系;叉鞭金藻中MDA含量在较低Sr^2 浓度下稍呈下降趋势,但与对照组相比并无显著差异,而在23.04mmol/L Sr^2 浓度时是对照的1.25倍;低Sr^2 浓度时,叉鞭金藻中蛋白质含量没有大的变化,当浓度超过5.76mmol/L时则引起蛋白质含量下降;叉鞭金藻中抗氧化酶SOD在低Sr^2 时分别降低12.5%、7.5%和9.5%,高Sr^2 时显著升高,为对照组的85.7%;CAT、GPX活性在低Sr^2 浓度时差别不大,但当浓度为23.04mmol/L时升高显著,比对照组分别增加了19.8%和74.4%。 相似文献