Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress

Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.     

Seismic Inference using Genetic Algorithms

A flood of reliable seismic data will soon arrive. The migration to largertelescopes on the ground may free up 4-m class instruments for multi-sitecampaigns, and several forthcoming satellite missions promise to yieldnearly uninterrupted long-term coverage of many pulsating stars. We willthen face the challenge of determining the fundamental properties of thesestars from the data, by trying to match them with the output of ourcomputer models. The traditional approach to this task is to make informedguesses for each of the model parameters, and then adjust them iterativelyuntil an adequate match is found. The trouble is: how do we know that oursolution is unique, or that some other combination of parameters will notdo even better? Computers are now sufficiently powerful and inexpensivethat we can produce large grids of models and simply compare all ofthem to the observations. The question then becomes: what range ofparameters do we want to consider, and how many models do we want tocalculate? This can minimize the subjective nature of the process, but itmay not be the most efficient approach and it may give us a false sense ofsecurity that the final result is correct, when it is really justoptimal. I discuss these issues in the context of recent advances inthe asteroseismological analysis of white dwarf stars.     

蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。     

Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis

INTRODUCTIONTriploidanimalsareusefulbecausetheyaresterile ,characterizedbypositivegrowthinthere productiveseason ,usefulfortesting ,forintroductionofnon nativespecies ,andforprotectionofde velopedstrains.Theinductionoftriploidyhadbeenreportedinmanyaquaculturespecies .Triploidshavebeensuccessfullyinducedinshrimpusingtemperatureshockorchemicalshock (CB ,6 DMAP)in 4species :Sicyoniaingentis (Xiangetal.,1 991 ) ,Fenneropenaeuschinensis (Xiangetal.,1 992 ;Daietal.,1 993;Baoetal.,1 993;Li…     

Gravitational wave radiation from the coalescence of white dwarfs

We compute the emission of gravitational radiation from the merging of a close white dwarf binary system. This is done for a wide range of masses and compositions of the white dwarfs, ranging from mergers involving two He white dwarfs, through mergers in which two CO white dwarfs coalesce, to mergers in which a massive ONe white dwarf is involved. In doing so we follow the evolution of the binary system using a smoothed particle hydrodynamics code. Even though the coalescence process of the white dwarfs involves considerable masses, moving at relatively high velocities with a high degree of asymmetry we find that the signature of the merger is not very strong. In fact, the most prominent feature of the coalescence is that in a relatively small time-scale (of the order of the period of the last stable orbit, typically a few minutes) the sources stop emitting gravitational waves. We also discuss the possible implications of our calculations for the detection of the coalescence within the framework of future space-borne interferometers like LISA.     

Galileo系统及其在中国的应用

较详细地介绍了Galileo系统的全面体系结构、频率设计、服务内容及其广泛的应用范围，指出了它与GPS系统相比的优点，并对开发和增强Galileo系统在我国多层次、全方位的应用提出了建议。