Silicon limitation on primary production and its destiny in Jiaozhou Bay, China——Ⅳ：Study on cross-bay transect from estuary to ocean

The authors analyzed the data collected in the Ecological Station Jiaozhou Bay from May 1991 to November 1994, including 12 seasonal investigations, to determine the characteristics, dynamic cycles and variation trends of the silicate in the bay. The results indicated that the rivers around Jiaozhou Bay provided abundant supply of silicate to the bay. The silicate concentration there depended on river flow variation. The horizontal variation of silicate concentration on the transect showed that the silicate concentration decreased with distance from shorelines. The vertical variation of it showed that silicate sank and deposited on the sea bottom by phytoplankton uptake and death, and zooplankton excretion. In this way, silicon would endlessly be transferred from terrestrial sources to the sea bottom. The silicon took up by phytoplankton and by other biogeochemical processes led to insufficient silicon supply for phytoplankton growth. In this paper, a 2D dynamic model of river flow versus silicate concentration was established by which silicate concentrations of 0.028–0.062 μmol/L in seawater was yielded by inputting certain seasonal unit river flows (m³/s), or in other words, the silicate supply rate; and when the unit river flow was set to zero, meaning no river input, the silicate concentrations were between 0.05–0.69 μmol/L in the bay. In terms of the silicate supply rate, Jiaozhou Bay was divided into three parts. The division shows a given river flow could generate several different silicon levels in corresponding regions, so as to the silicon-limitation levels to the phytoplankton in these regions. Another dynamic model of river flow versus primary production was set up by which the phytoplankton primary production of 5.21–15.55 (mgC/m²·d)/(m³/s) were obtained in our case at unit river flow values via silicate concentration or primary production conversion rate. Similarly, the values of primary production of 121.98–195.33 (mgC/m²·d) were achieved at zero unit river flow condition. A primary production conversion rate reflects the sensitivity to silicon depletion so as to different phytoplankton primary production and silicon requirements by different phytoplankton assemblages in different marine areas. In addition, the authors differentiated two equations (Eqs. 1 and 2) in the models to obtain the river flow variation that determines the silicate concentration variation, and in turn, the variation of primary production. These results proved further that nutrient silicon is a limiting factor for phytoplankton growth. This study was funded by NSFC (No. 40036010), and the Director's Fund of the Beihai Sea Monitoring Center, the State Oceanic Administration.     

盐泽螺旋藻变种的生长和生化特性

采用１９８４年在美国加利福尼亚大学培育成功的盐泽螺旋藻变种，经扫描电镜，生物量测定和生化分析，进一步研究其基本特性。结果表明，其藻丝呈紧密的逆时针向等宽螺旋结构。     

胶州湾海域水质预测

在已建潮流模型的基础上,用ADI法建立了胶州湾扩大域变动边界的平流—扩散输运模型,以COD为指示因子,预测了胶州湾海域1995年、2000年的水质、青岛环海公路海上段、沧口区北半部污水截流、北水南调、集中排放以及胶州湾西部经济开发对海域水质的影响等。为青岛市环保部门制定环保规划提供决策依据。     

天然水体中酚的降解动力学

本文报道了天然水体中低浓度酚的降解动力学;计算了它的降解速率常数、降解活化能等。降解半表期是t_(1/2)=1.5d~(-1)(10℃),t_(1/2)=O.74d~(-1)(30℃)。低浓度酚的降解动力学跟天然水体中酚的降解情况比较接近。本文的结果对环境评价、蓄水库工程设计等很有参考价值。     

中华乌塘鳢的群体生物学研究 Ⅱ.生化遗传变异

用同工酶谱法发现中华乌塘鳢Bostrichthy sinensis四个群体的遗传变异水平很低,在所检测的30个基因座位中,多态座位比例只有0—10％,平均杂合度仅为0.2—0.5％,物种的平均杂合度为0.33±0.10％。四个群体共享常见的等位基因。虽然其中三个群体拥有特有的等位基因,但其基因频率都未超过5％。四个群体的Nei氏遗传距只有1.68—12.13×10~(-5)。据分析,近交可能是遗传变异水平低和形态特征分化差的主要原因。种苗生产上应注意广泛采集不同地理群体的鱼作亲鱼,以丰富养殖群体的基因库。     

几种养殖鲍同工酶生化遗传的比较研究

运用聚丙烯酰胺凝胶电泳对我国主要养殖鲍(皱纹盘鲍、盘鲍及九孔鲍)12种同工酶(LDH、ME、MDH、GPI、PGM、IDH、IDDH、SOD、EST、ODH、ADH、AAT)26个基因座位进行了分析．结果表明：EST、PGM可以作为区分三种(亚种)鲍的生化遗传标记；在所分析的位点中，未发现多态现象，表明三种养殖鲍杂合子缺失严重，其主要原因可能在于哑基因、近交与自然选择。     

秦山核电站邻近水域石油降解细菌的生态学研究

本文分析了1989年4月(春),7月(夏),10月(秋)和1990年1月(冬)秦山邻近水域表层水中石油降解细菌的丰度和分布规律,同时比较了温度、含油量、营养盐、溶解氧、盐度等环境因素对其数量变动的影响。分析结果发现,测区表层水中石油降解菌数量最高值达1.1×10~5个/dm~3,最低值为400个/dm~3,季节变化波动于97～4.9×10~4个/dm~3之间,菌量变化与水温,含油量呈正相关,与盐度呈负相关趋势,认为测区高营养盐、高溶解氧状况可能不成为石油降解细菌生长繁殖的限制因子,并指出测区低丰度的石油降解菌数量与该海区低水平的油污是一致的。     

红色无硫细菌处理高浓度鱼品废水的研究

本文从城市生活污水的沉降污泥中分离出三株红色无硫细菌,经鉴定R1为胶质红假单胞菌(Rhodopscudomonas gelatinosa),R2为沼泽红假单胞菌(R palustris)、RS-1为红螺属(Rhodosprillum)的一种。R1与R2均为兼性需氧和兼性光能利用菌,RS-1为专性厌氧光合菌。作者提出了它们的主要生理、生化特性和最适生长条件,最后确定R1、R2是较有利用前途的优势种类。作者利用上述分离的菌株对高浓度的鱼品废水进行了净化处理试验,得出R1对CODcr的去除率约为65％,R2的去除率为74％以上。二者混合使用可提高至87％。作者还发现废水中的非光合性杂生菌有助于提高光合细菌的净化力。     

黄鳍鲷LDH同工酶的组织特异性研究

以黄鳍鲷为材料进行两方面研究:（1）用聚丙烯酰胺凝胶电泳方法，对其肌肉，心脏，肝脏，血清，玻璃体，晶状体，脑等组织器官进行LDH同工酶分析比较，结果表明，不同组织中LDH的同工酶谱带不相同，存在着组织特异性：（2）用聚丙烯酰胺等电聚焦电泳法对其肌肉，血清的LDH同工酶进行分离 ，结果得到12－15条电泳区带，等电点范围在pH为3．65－9．58之间。     

黄河口附近海域化学需氧量和石油烃分布及其关键控制环境因子分析

于2009年12月、2010年5月、2011年9月、2013年5月和2014年10月,在黄河口附近海域进行了5个航次调查,研究了该海域的化学需氧量(COD)和石油烃的分布情况以及季节变化。结果表明,COD整体呈冬季低、春季高的趋势,而石油烃无明显季节变化趋势。平面分布上,春季和秋季COD高值主要分布在靠近黄河口海域。春季和冬季石油烃高值主要位于调查海域的东北部区域。相关分析结果表明盐度、温度、pH、悬浮物、溶解氧及磷酸盐是影响COD的主要环境因素,而石油烃主要受盐度、温度、pH、无机氮、磷酸盐的影响。进一步多元回归分析结果表明,p H、盐度和溶解氧是影响COD的关键因子,温度是影响石油烃的关键因子。