Intracrystalline lipids within sulfates from the Haughton Impact Structure—Implications for survival of lipids on Mars

Lipids can be present within gypsum as intracrystalline inclusions if they become incorporated within the mineral as is it precipitates. The lipids that comprise these inclusions are protected against alteration or destruction by an external oxidising chemical environment because a protective mineral matrix surrounds them. Sulfate minerals are abundant on the surface of Mars and were present in the samples that were analysed by the Viking landers. The quantities of secondary intracrystalline fossil-lipids that are present in samples of gypsum and gypsum-rich soils from the Haughton Impact Structure, Devon Island, Canadian High Arctic are sufficient to suggest that if a similar concentration of fossil lipids was present in the sulfate-rich samples analysed by the Viking Landers then they could have been detected. Possible reasons why a secondary fossil-lipid signature was not detected include a poor rate of conversion during pyrolysis, exposure of intracrystalline lipids during periods of weathering to oxidative martian diagenesis, a low level of biological productivity or an absence of a source for lipids on the surface of Mars. Polycyclic aromatic hydrocarbons of meteoritic origin, and terpane biomarkers such as hopanes and steranes, are not present in the Haughton gypsum in sufficient quantities to have been readily detected.     

Methane evolution from UV-irradiated spacecraft materials under simulated martian conditions: Implications for the Mars Science Laboratory (MSL) mission

Fifteen organic and three inorganic compounds were tested for methane (CH₄) evolution under simulated martian conditions of 6.9 mbar; UVC (200-280 nm) flux of 4 W m⁻²; 20 °C; simulated optical depth of 0.1; and a Mars gas composition of CO₂ (95.3%), N₂ (2.7%), Ar (1.7%), O₂ (0.13%), and water vapor (0.03%). All three inorganic compounds (i.e., NaCl, CaCO₃, graphite) failed to evolve methane at the minimum detection level 0.5 ppm, or above. In contrast, all organic compounds evolved methane when exposed to UV irradiation under simulated martian conditions. The polycyclic aromatic hydrocarbon, pyrene, released the most methane per unit of time at 0.175 nmol CH₄ g⁻¹ h⁻¹, and a spectral reflectance target material used for the MER rovers and Phoenix lander released the least methane at 0.00065 nmol CH₄ cm⁻² h⁻¹. Methane was also released from UV-killed bacterial endospores of Bacillus subtilis. Although all organic compounds evolved methane when irradiated with UV photons under martian conditions, the concentrations of residual organics, biogenic signature molecules, and dead microbial cells should be relatively low on the exterior surfaces of the MSL rover, and, thus, not significant sources of methane contamination. In contrast, kapton tape was found to evolve methane at the rate of 0.00165 nmol CH₄ cm⁻² h⁻¹ (16.5 nmol m⁻² h⁻¹) under the UV and martian conditions tested. Although the evolution of methane from kapton tape was found to decline over time, the large amount of kapton tape used on the MSL rover (lower bound estimated at 3 m²) is likely to create a significant source of terrestrial methane contamination during the early part of the mission.     

Abodes for life in carbonaceous asteroids?

Thermal evolution models for carbonaceous asteroids that use new data for permeability, pore volume, and water circulation as input parameters provide a window into what are arguably the earliest habitable environments in the Solar System. Plausible models of the Murchison meteorite (CM) parent body show that to first-order, conditions suitable for the stability of liquid water, and thus pre- or post-biotic chemistry, could have persisted within these asteroids for tens of Myr. In particular, our modeling results indicate that a 200-km carbonaceous asteroid with a 40% initial ice content takes almost 60 Myr to cool completely, with habitable temperatures being maintained for ∼24 Myr in the center. Yet, there are a number of indications that even with the requisite liquid water, thermal energy sources to drive chemical gradients, and abundant organic “building blocks” deemed necessary criteria for life, carbonaceous asteroids were intrinsically unfavorable sites for biopoesis. These controls include different degrees of exothermal mineral hydration reactions that boost internal warming but effectively remove liquid water from the system, rapid (1-10 mm yr⁻¹) inward migration of internal habitable volumes in most models, and limitations imposed by low permeabilities and small pore sizes in primitive undifferentiated carbonaceous asteroids. Our results do not preclude the existence of habitable conditions on larger, possibly differentiated objects such as Ceres and the Themis family asteroids due to presumed longer, more intense heating and possible long-lived water reservoirs.     

Thermal contraction crack polygons on Mars: A synthesis from HiRISE, Phoenix, and terrestrial analog studies

Thermal contraction crack polygons are complex landforms that have begun to be deciphered on Earth and Mars by the combined investigative efforts of geomorphology, environmental monitoring, physical models, paleoclimate reconstruction, and geochemistry. Thermal contraction crack polygons are excellent indicators of the current or past presence of ground ice, ranging in ice content from weakly cemented soils to debris-covered massive ice. Relative to larger topographic features, polygons may form rapidly, and reflect climate conditions at the time of formation—preserving climate information as relict landforms in the geological record. Polygon morphology and internal textural characteristics can be used to distinguish surfaces modified by the seasonal presence of a wet active layer or dry active layer, and to delimit subsurface ice conditions. Analysis of martian polygon morphology and distribution indicates that geologically-recent thermal contraction crack polygons on Mars form predominantly in an ice-rich latitude-dependent mantle, more likely composed of massive ice deposited by precipitation than by cyclical vapor diffusion into regolith. Regional and local heterogeneities in polygon morphology can be used to distinguish variations in ice content, deposition and modification history, and to assess microclimate variation on timescales of ka to Ma. Analyses of martian polygon morphology, guided by investigations of terrestrial analog thermal contraction crack polygons, strongly suggest the importance of excess ice in the formation and development of many martian thermal contraction crack polygons—implying the presence of an ice-rich substrate that was fractured during and subsequent to obliquity-driven depositional periods and continually modified by ongoing vapor equilibration processes.     

Evidence for pervasive mud volcanism in Acidalia Planitia, Mars

We have mapped 18,000+ circular mounds in a portion of southern Acidalia Planitia using their sizes, shapes, and responses in Nighttime IR. We estimate that 40,000+ of these features could occur in the area, with a distribution generally corresponding to the southern half of the proposed Acidalia impact basin. The mounds have average diameters of about 1 km and relief up to 180 m and most overlie units mapped as Early Amazonian.High resolution images of mound surfaces show relatively smooth veneers, apron-like extensions onto the plains, moats, and concentric circular crestal structures. Some images show lobate and flow-like features associated with the mounds. Albedo of the mounds is generally higher than that of the surrounding plains. Visible and near-infrared spectra suggest that the mounds and plains have subtle mineralogical differences, with the mounds having enhanced coatings or possibly greater quantities of crystalline ferric oxides.Multiple analogs for these structures were assessed in light of new orbital data and regional mapping. Mud volcanism is the closest terrestrial analogy, though the process in Acidalia would have had distinctly martian attributes. This interpretation is supported by the geologic setting of the Acidalia which sits at the distal end of the Chryse-Acidalia embayment into which large quantities of sediments were deposited through the Hesperian outflow channels. In its distal position, Acidalia would have been a depocenter for accumulation of mud and fluids from outflow sedimentation.Thus, the profusion of mounds in Acidalia is likely to be a consequence of this basin’s unique geologic setting. Basinwide mud eruption may be attributable to overpressure (developed in response to rapid outflow deposition) perhaps aided by regional triggers for fluid expulsion related to events such as tectonic or hydrothermal pulses, destabilization of clathrates, or sublimation of a frozen body of water. Significant release of gas may have been involved, and the extensive mud volcanism could have created long-lived conduits for upwelling groundwaters, providing potential habitats for an in situ microbiota.Mud volcanism transports minimally-altered materials from depth to the surface, and mud volcanoes in Acidalia, therefore, could provide access to samples from deep zones that would otherwise be inaccessible. Since the distal setting of Acidalia also would favor concentration and preservation of potentially-present organic materials, samples brought to the surface by mud volcanism could include biosignatures of possible past or even present life. Accordingly, the mounds of Acidalia may offer a new class of exploration target.     

Lava tubes and basaltic caves as astrobiological targets on Earth and Mars: A review

Lava tubes and basaltic caves are common features in volcanic terrains on Earth. Lava tubes and cave-like features have also been identified on Mars based on orbital imagery and remote-sensing data. Caves are unique environments where both secondary mineral precipitation and microbial growth are enhanced by stable physico-chemical conditions. Thus, they represent excellent locations where traces of microbial life, or biosignatures, are formed and preserved in minerals. By analogy with terrestrial caves, caves on Mars may contain a record of secondary mineralization that would inform us on past aqueous activity. They may also represent the best locations to search for biosignatures. The study of caves on Earth can be used to test hypotheses and better understand biogeochemical processes, and the signatures that these processes leave in mineral deposits. Caves may also serve as test beds for the development of exploration strategies and novel technologies for future missions to Mars. Here we review recent evidence for the presence of caves or lava tubes on Mars, as well as the geomicrobiology of lava tubes and basaltic caves on Earth. We also propose future lines of investigation, including exploration strategies and relevant technologies.     

The shielding effect of small-scale martian surface geometry on ultraviolet flux

The atmosphere of Mars does little to attenuate incoming ultraviolet (UV) radiation. Large amounts of UV radiation sterilize the hardiest of terrestrial organisms within minutes, and chemically alter the soil such that organic molecules at or near the surface are rapidly destroyed. Thus the survival of any putative martian life near the surface depends to a large extent on how much UV radiation it receives. Variations in small-scale geometry of the surface such as pits, trenches, flat faces and overhangs can have a significant effect on the incident UV flux and may create “safe havens” for organisms and organic molecules. In order to examine this effect, a 1-D radiative transfer sky model with 836 meshed points (plus the Sun) was developed which includes both diffuse and direct components of the surface irradiance. This model derives the variation of UV flux with latitude and an object's Geometric Shielding Ratio (a ratio which describes the geometry of each situation). The best protection is offered by overhangs with flux reduced to a factor of 1.8±0.2×10⁻⁵ of the unprotected value, a reduction which does not vary significantly by latitude. Pits and cracks are less effective with a reduction in UV flux of only up to 4.5±0.5×10⁻³ for the modeled scenarios; however, they are more effective for the same geometric shielding ratio than overhangs at high latitudes due to the low height of the Sun in the sky. Lastly, polar faces of rocks have the least effective shielding geometry with at most a 1.1±0.1×10⁻¹ reduction in UV flux. Polar faces of rocks are most effective at mid latitudes where the Sun is never directly overhead, as at tropical latitudes, and never exposes the back of the rock, as at polar latitudes. In the most favorable cases, UV flux is sufficiently reduced such that organic in-fall could accumulate beneath overhanging surfaces and in pits and cracks. As well, hardy terrestrial microorganisms such as Bacillus pumilus could persist for up to 100 sols on the outer surfaces of typical spacecraft or several tens of martian years in the most shielded surface niches.     

Preparing For Hyperseed MAC: An Observing Campaign To Monitor The Entry Of The Genesis Sample Return Capsule

The imminent return of the Genesis Sample Return Capsule (SRC) from the Earth’s L1 point on September 8, 2004, represents the first opportunity since the Apollo era to study the atmospheric entry of a meter-sized body at or above the Earth’s escape speed. Until now, reentry heating models are based on only one successful reentry with an instrumented vehicle at higher than escape speed, the 22 May 1965 NASA “FIRE 2” experiment. In preparation of an instrumented airborne and ground-based observing campaign, we examined the expected bolide radiation for the reentry of the Genesis SRC. We find that the expected emission spectrum consists mostly of blackbody emission from the SRC surface (T∼ ∼2630 K@peak heating), slightly skewed in shape because of a range of surface temperatures. At high enough spectral resolution, shock emission from nitrogen and oxygen atoms, as well as the first positive and first negative bands of N₂⁺, will stand out above this continuum. Carbon atom lines and the 389-nm CN band emission may also be detected, as well as the mid-IR 4.6-μm CO band. The ablation rate can be studied from the signature of trace sodium in the heat shield material, calibrated by the total amount of matter lost from the recovered shield. A pristine collection of the heat shield would also permit the sampling of products of ablation.     

Microbial signatures in sabkha evaporite deposits of Chott el Gharsa (Tunisia) and their astrobiological implications

This study investigates the geomicrobiological potential of Upper Pleistocene evaporite deposits of the Chott el Gharsa, a wide continental sabkha in southern Tunisia. Organic and inorganic-derived biosignatures are mostly contained in microcrystalline, laminated gypsum lithofacies consisting of light/dark alternations of concordant laminae, which have precipitated from high salt concentrated waters. These biosignatures include mineralized microbial-interpreted morphologies, such as mucilage, rods, and microfibers, and dumbbell morphologies in the hollow cores of dolomite crystals that are associated with sulfates. Mineral products that are induced by microbial activity and their organic compounds lead to the formation of lenticular-shaped gypsum crystals, with a high length/width ratio, dolomite precipitation and formation of pyrite framboids. Morphological and structural aspects of these biosignatures, and their composition, in laminated, dolomite-rich sulfate deposits could be detected through microscopic investigations and micro-analyses performed by the instrumentation that is planned for ongoing Mars sample return missions.     

Transport of ionizing radiation in terrestrial-like exoplanet atmospheres

The propagation of ionizing radiation through model atmospheres of terrestrial-like exoplanets is studied for a large range of column densities and incident photon energies using a Monte Carlo code we have developed to treat Compton scattering and photoabsorption. Incident spectra from parent star flares, supernovae, and gamma-ray bursts are modeled and compared to energetic particles in importance. Large irradiation events with fluences of 10⁶-10⁹ erg cm⁻² at the conventional habitable zone can occur at a rate from many per day (flares from young low-mass parent stars) to ∼100 per Gyr (supernovae and gamma-ray bursts). We find that terrestrial-like exoplanets with atmospheres thinner than about 100 g cm⁻² block nearly all X-rays, but transmit and reprocess a significant fraction of incident γ-rays, producing a characteristic, flat surficial spectrum. Thick atmospheres (?100 g cm⁻²) efficiently block even γ-rays, but nearly all the incident energy is redistributed into diffuse UV and visible aurora-like emission, increasing the effective atmospheric transmission by many orders of magnitude. Depending on the presence of molecular UV absorbers and atmospheric thickness, up to 10% of the incident energy can reach the surface as UV reemission. For the Earth, between 2×10⁻³ and 4×10⁻² of the incident flux reaches the ground in the biologically effective 200-320 nm range, depending on O₂/O₃ shielding. For atmospheres thicker than ∼50 g cm⁻² in the case of pure Rayleigh scattering and ∼100 g cm⁻² in the case of O₂/O₃ absorption, the UV reemission exceeds the surficial transmitted ionizing radiation. We also discuss the effects of angle of incidence and derive a modified two-stream approximation solution for the UV transfer. Finally, we suggest that transient atmospheric ionization layers can be frequently created at altitudes lower than the equilibrium layers that result from steady irradiation and winds from the parent star. We suggest that these events can produce frequent fluctuations in atmospheric ionization levels and surficial UV fluxes on terrestrial-like planets.