浙江枝吻纽虫（Dendrorhynchus zhejiangensis）凝溶胶蛋白和肌动蛋白基因的克隆与序列分析

利用已构建的浙江枝吻纽虫cDNA文库,通过PCR技术扩增得到gelsolin和actin基因的全长cDNA序列。其中,gelsolin的cDNA全长1947bp,5′-非翻译区62bp,3′-非翻译区778bp,开放阅读框1107bp,编码369个氨基酸;actin的cDNA全长为1830bp,5′-非翻译区167bp...     

Oxygen radicals production and actin filament disruption in bivalve haemocytes treated with benzo(a)pyrene

Haemocytes play an essential role in the internal defence of molluscs. It has been reported that organic xenobiotics commonly found as pollutants in the marine environment impair defence capabilities of haemocytes. The purpose of the present study was to investigate the effects of benzo(a)pyrene [B(a)P] on the integrity of the actin cytoskeleton and on endocytosis in haemocytes and to see if these effects are related to generation of reactive oxygen species. Haemocytes were exposed in vitro to B(a)P (0.5–40 μg/ml) for 1 h. Cell viability (using 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide or XTT assay) indicated that selected doses were sublethal. Uptake of neutral red was significantly decreased in a dose-dependent manner in B(a)P-treated haemocytes. Distribution of actin filaments, labeled with rhodamine-conjugated phalloidin, was altered in haemocytes treated with 20 or 40 μg/ml B(a)P. These effects could be related to an increased production of superoxide anion during B(a)P metabolism, as detected by the nitroblue tetrazolium (NBT) reduction assay in haemocytes treated with 10 μg/ml B(a)P.     

微丝解聚剂及微管阻断剂对藓羽藻细胞重建过程的影响

某些种类的海洋多核绿藻的原生质被挤出后,其细胞器在人工海水中可立即发生聚集,随后其细胞组分发生重建,形成新的细胞膜与细胞壁,这些重建的细胞在最佳培养条件下,最多有40%能够发育成成熟藻体。细胞骨架系统是细胞内有序性的维系者,也是细胞及细胞器运动的动力源,这些过程都涉及细胞骨架的解聚与重聚合的动态过程。本文利用微丝解聚剂细胞松弛素B及微管阻断剂秋水仙素对藓羽藻(Bryopsis hypnoides)细胞重建过程的影响进行了研究。结果显示,细胞器聚集及聚集体变圆的动力不来自于细胞骨架系统,而原始微丝体系的解聚是细胞器高效聚集的基本条件;细胞松弛素B及秋水仙素都显著抑制细胞膜的形成及细胞内组分有序性重建等后继发育过程,表明细胞骨架系统在这些过程中扮演着重要角色。