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为研究青鱼暴发性死亡原因,从病症典型青鱼(Mylopharyngodon piceus)肌肉、肝脏和肾脏处进行细菌接种,分离获得一株细菌(HZ2015),对其进行了形态观察、生化特性检测和16S r RNA序列分析,通过动物回归实验、毒力检测和药敏试验了解其对青鱼的致病性、毒力及药物敏感性。所得菌株为类志贺邻单胞菌(Plesiomonas shigelloides),动物回归实验能复制出类似症状,且能从体内再次分离到该菌株;分离株对小鼠的半数致死量(LD50)为106.6 CFU;对头孢呋辛、头孢哌酮、庆大霉素、卡那霉素、复达欣高度敏感。研究表明,菌株HZ2015为青鱼体表溃烂病病原,本研究系类志贺邻单胞菌致青鱼体表溃烂病的首次报道。 相似文献
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青鱼为海产冷水性中上层鱼,属于硬骨鱼类的鲱鱼科,学名:太平洋鲱鱼(Clupea pullasi Cuv.&Val)当地俗称青鱼,其体形小而侧扁,背部为深灰蓝色,具有背鳍、胸鳍、臀鳍、尾鳍为正尾形。一般年成,青鱼个体质量多为150-200g。20世纪70-80年代,鱼群中偶尔有约250g/条的青鱼(极少数),属中上等鱼,肉质极富油性。每年2-6月份洄游至我国黄海北部各湾产卵。 相似文献
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Ustadi K. Y. Kim S. M. Kim 《中国海洋大学学报(英文版)》2005,4(3):198-204
The protease inhibitor was purified from five different fish eggs. The molecular weights of Pacific herring, chum salmon, pond smelt, glassfish, and Alaska pollock egg protease inhibitors were 120, 89, 84.5, 17, and 16.8kDa, respectively. The specific inhibitory activity of glassfish egg protease inhibitor was the highest followed by those of Pacific herring and Alaska pollock in order. The specific inhibitory activity and purity of glassfish egg protease inhibitor were 19.70 U mg^- 1 protein and 164.70 folds of purification, respectively. Glassfish egg protease inhibitor was reasonably stable at 50 - 65℃ and pH 8, which was more stable at high temperature and pH than protease inhibitors from the other fish species. Glassfish egg protease inhibitor was noncompetitive with inhibitor constant (Ki) of 4.44 nmol L^-1 相似文献
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