光强度对附着的玻片上的幼鲍饵料生物的影响

幼鲍初期饵料生物在玻片上着生物量的消长与光强度的关系进行了试验，光强度超过２５００Ｌｕ时微生物膜增长较快，膜脱落较早；光强度低于１０００Ｌｕｘ时微生物膜净生产量较低，光强度在１５００－２０００Ｌｕｘ时有利于膜生物量的积累，舟形藻和菱形藻的纯培养在玻片上附着的生物量呈稳定性高速增长并能延迟生物膜脱落现象的发生。     

EFFECT OF LIGHT INTENSITY ON THE TOTAL LIPID AND FATTY ACID COMPOSITION OF SIX STRAINS OF MARINE DIATOMS

The effect of light intensity ( 1500 Ix and 5000 Ix) on the total lipid and fatty acid composition of six strains of marine diatoms Cylindrotheca fusiformis (B211 ), Phaeodactylum tricornutum (Bl14, Bl18 and B221) Nitzschia closterium (B222) and Chaetoceros gracilis (B13) was investigated.The total lipids of B13, Bl14, and B211 grown at 5000 Ix were lower than those grown at 1500 Ix. No evident changes were observed in Bl18, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14:0, 16:0, 16:l(n- 7) and 20:5(n-3) . Cylindrothecafusiformis had high percentage of 20:4n - 6 (9.2 - 10.9% ) . The total polyunsaturated fatty acid /rl all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains except Chaetoceros gracilis.     

A New Combination and Two Varieties of Polystigmate Gomphonema （Gomphonemaceae Bacillariophyta） from Heilongjiang Province, China

The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema tuttis Ehr. vat. latum Fan et Wang. var.nov. and Gomphonema acum/natum var. obtusum Fan et Bao, var. nov., that were distinguished by frustule morphology under LM. The samples were collected from freshwater of Heilongjiang Province, China.     

Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.     

西沙群岛附近海域羽纹硅藻分类研究 Ⅰ

继“西沙、中沙群岛附近海域浮游硅藻类分类的研究”之后,作者又对西沙群岛所属岛、礁附近海域的羽纹硅藻进行了初步整理,共鉴定出羽纹硅藻11属27种及其变种(其中8种是我国首次记录)。本文对首次记录的种类进行了比较详细的说明和绘图。某些在我国其他海域已有报道的种类,如光辉针杆藻、串珠梯楔形藻、海生斑条藻、琴状舟形藻、流苏胸隔藻、双标胸隔藻、钝端双眉藻、细纹斜盘藻等,以往的描述较简单,并且与西沙群岛标本的形态也不完全雷同,为了便于应用和比较,本文根据西沙群岛的标本,又进行了较详细的描述或补充说明。对有图而无说明,或仅有名录的种类的国内资料,在地理分布内注明。为了保持西沙群岛所属海城硅藻分类研究的连续性,本文仍采用 Hendey 提出的分类系统排列。 为便于读者査找,每一种名下列出其同物异名。 标本多用小型浮游生物网采得,有些则取自珊瑚表面。插图均用描图仪描绘。     

中华半管藻繁殖生物学研究──Ⅱ.营养细胞和休眼孢子的形态结构

通过扫描电镜观察中华半管藻的营养细胞和休眠孢子的形态结构显示：营养细胞上下壳各具一个线性裂口而不外突的唇形突,壳壁上的眠纹放射状,爪状连接刺上有零散的单孔,从单孔分泌胶质与相邻细胞连接;休眠孢子双凸透镜形或近球形,壳厚,表面平滑无纹饰,上壳面边缘具8～16根分叉长刺,下壳面具微刺,外围有一裙状物,其上有微孔。两类细胞不同的形态特征反映其不同的生态适应。     

西沙群岛海洋硅藻的研究：Ⅰ.双眉藻属AmphoraEhrenberg二新种…

在西沙群岛潮间带底栖硅藻的研究中发现了２个新种。Ａｍｐｈｏｒａ ｐｕｎｃｔａｔａ．ｎｏｖ，Ａｍｐｈｏｒａｍｉｃｒｏｍｅｔｒｏｉｄｅｓｓｐ．ｎｏｖ，１个待定种和Ａｍ．ａｆ．ｃａｓｔｅｌｌａｔａ等１４个新记录。     

锯缘青蟹NAGase的催化机理的判断

以p-硝基苯酚-β-D-氨基葡萄糖苷（pNP-NAG）为底物，研究产物类似物：p-羧基苯酚和苯酚对青蟹N-乙酰-β-D-氨基葡萄糖苷酶（NAGase，Ec3．2．1．52）活力的影响．结果表明：p-羧基苯酚和苯酚对该酶有抑制的作用，IC50分别为20．0和100．0mmol／dm^3．p-羧基苯酚和苯酚对酶的抑制均表现为竞争性类型，其抑制常数K1分别为5．03和25．67mmol／dm^3．结合产物N-乙酰-β-D-氨基葡萄糖（NAG）抑制作用表现为反竞争性类型，判断NAGase水解pNP-NAG反应为有序双双反应（Ordered Bi Bi）．     

南海晚第四纪的硅藻遗体及其地质意义：V.沟纹藻属

硅藻门沟纹藻属在我国是１个新记录的属。核属最先由Ｋｏｌｂｅ（１９５４）在西太平洋赤道区（２°５２＇Ｓ，８９°５０＇Ｗ，水深３２２５ｍ）的柱状样沉积物中找到１种，订名Ｌ．ｐｅｔｔｅｒｓｓｏｎｉｉ。之后，又在１９５５年从大西洋（０°７＇Ｎ，１９°１２＇ｅ；０°８′Ｎ，１６°１９′Ｅ）和太平洋（６°４４′Ｎ，１２９°２８′Ｗ；２°５２′Ｓ，８９°５′Ｗ）的柱状样沉积物中找到另１种，订名Ｌ．ｈｕｓｔｅｄｔ     

龙感湖地区近3000年来的植被及其气候定量重建

本文依据龙感湖钻孔的孢粉及硅藻资料，探讨了该地区近３０００年来的植被发展及气候变化，通过数值分析方法，重建了年均温及年降水的时间序列，发现气候波动存在的两个阶段，（依１．５ｋａＢ．Ｐ．前后划分）四个旋回（０．８ｋａ的准周期）两个突变事件（３ｋａＢ．Ｐ）前后的冷暖急剧变化及１ｋａＢ．Ｐ的降温事件）。这些变化与敦德冰心及固城湖孢粉反映的气候波动有较好的一致性，具有全球变化意义。