Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress

Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.     

海水中致病菌检测研究进展

海水中致病菌种类繁多、致病性强,对水产养殖和人体健康存在着潜在危害。传统的检测方法培养过程复杂繁琐、耗时长、效率低,难以满足实际工作的需求。因此,建立海水中致病菌的快速、灵敏、准确的检测技术势在必行。本文综述了流式细胞技术、分子生物学方法、免疫学方法和生物传感器技术等在海水致病菌检测中的应用,总结了上述方法在海水致病菌检测中的研究现状。     

虾类免疫学的基础和应用研究

无脊椎动物免疫学是一门新兴学科 ,甲壳动物免疫学是其中的一个分支［１］。自６０年代后期以来 ,瑞典、美国、日本等国学者发表了大量的论文及专著 ,对甲壳动物免疫机制等问题进行了有益的探索。近十余年来 ,甲壳动物免疫学发展较快 ,正在逐渐形成一门新的学科分支 ,引起国内外学者的高度重视 ,其中虾类免疫学发展最快。鉴于目前虾类病害防治不理想的现状 ,可以认为虾类免疫学的研究是最终战胜虾类病害的重要基础。本文力图对该领域的最新进展作一概述。１免疫系统的研究１．１免疫应答的淋巴器官和组织虾类免疫应答的主要组织和器官为淋巴…     

海水养殖动物弧菌病防治的研究进展

本文综述了近年来有关水产动物弧菌病防治研究的进展情况。抗菌药物仍然是目前控制水产动物弧菌病的主要手段，但药物残留和耐药菌的大量出现制约了其进一步的发展。免疫学方法和生态防治法在水产动物弧菌病防治方面已显示了良好的效果，具有广泛的应用和推广前景。     

皱纹盘鲍对河流弧菌-Ⅱ苗免疫的研究

于１９９３－１９９５年，用免疫法防治皱纹盘鲍脓疱病，河流弧菌－ＩＩ分离自大连水产养殖公司，太平洋海珍品养殖公司的患脓疱病的皱纹盘鲍；健康皱纹盘鲍，由大连水产养殖公司石庙苗种厂提供，鲍血淋巴细胞的体外培养吞噬实验，取健康皱纹盘鲍的血淋巴与肝素和菌液铵一定比例混合后，置湿盒内３７℃培养３０ｍｉｎ，取一滴涂片，吕氏液染色后观察，用该病原菌制做菌苗，大量扩增病原菌，用０．１％－１．０％的甲醛洗下菌苔，处理     

对虾白斑综合症病毒的诊断和检测

对虾白斑综合症病毒（White Spot Syndrome Virus，WSSV）也叫白斑综合症杆状病毒（WSBV）、系统外胚层和中胚层杆状病毒（SEMBV）、日本对虾杆状核病毒（RV-PJ）、对虾杆状DNA病毒（PRDV）、皮下及造血组织坏死杆状病毒（HHNBV）、中国对虾杆状病毒（PCBV）、淋巴样细胞核性杆状病毒（LNBV）（陈秀男等，1994；Wongteerasupaua等，1995；Inouye 等，1994、1996；黄倢等，1995a；战文斌等，1995；陈细法等，1997），是全球对虾养殖业危害性最大的病毒之一。自1992年WSSV暴发性流行以来，造成了对虾养殖业的严重萎缩。鉴…     

创伤弧菌疫苗对青石斑鱼的免疫学效应和安全性

弧菌病是海水网箱养殖石斑鱼Ｅｐｉｎｅｐｈｅｌｕｓ主要疾病之一。本文利用致病菌－创伤弧菌Ｖｉｂｒｉｏ ｖｕｌｎｉｆｉｃｕｓ强毒菌株制备出安全性很高的福尔马林灭活疫苗（ＦＶ）和热灭活疫苗（ＨＶ）。这两种疫苗无论使用佐剂与否，通过肌肉注射接种青石斑鱼Ｅ．ａｗｏａｒａ都能使鱼体在较短时间内产生较强的体液免疫应答，而且抗体效价能维持较长时间。免疫学的青石斑鱼获得了良好的免疫保护力。     

Histopathological Observation of Lymphocystis Disease and Lymphocystis Disease Virus （LCDV） Detection in Cultured Diseased Sebastes schlegeli

Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scat- tered in the cytoplasm, electron-dense particles 70-80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped parti- cles with electron-dense core of 70-80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues.