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排序方式: 共有197条查询结果,搜索用时 15 毫秒
1.
麻痹性贝毒(Paralytic Shellfish Poisoning,PSP)毒素由石房蛤毒素(saxitoxin,STX)及其衍生物组成,目前己发现20余种,在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止,限制了国内PSP毒素应用及研究的全面深入展开,因此本文作者对PSP毒素进行了制备,并采用不同方法对制备的PSP毒素进行了研究。
PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放,从而阻止神经冲动的发生和传导,使神经、肌肉丧失兴奋性(Frace et al.,1986;Penzotti et al.,1998)。本文利用神经束膜下记录和全细胞膜片钳技术,报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果,并与STX标准毒素的作用结果进行了比较。
NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞,经分化剂分化后,显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht,1977)和Na+、K+、Ca2+等多种离子通道,已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.,1992; Docherty et al.,1992;Shi et al.,1993;Hu et al.,1997a;Hu et al.,1997b);发育出具有Na+内流支持的锋电位(Hamprecht,1977),该电位是分析作用于膜钠离子通道药物的好材料。 相似文献
2.
据开发应用水产饲料膨化机实践,初步论述了膨化机生产能力与动力匹配,螺杆设计参数长径比,物料在腔内滞留时间,膨化腔设计及其温度调控,并提出改进设计的见解. 相似文献
3.
4.
通过成功地解决深圳市好湾电厂(二期)基础工程H型桩沉入困难问题,使用各种风动潜孔锤在巨石、中粗砂填海区进行了引孔钻进工艺的试验研究及应用,对风动潜孔锤钻进工艺及其机具的使用作了进一步的分析研究,提出了风动潜孔锤使用过程中工艺方面的改进措施。 相似文献
5.
Inclement weather creates a chronic hazard for Canadian travellers. Past studies indicate that road collision rates increase during precipitation, although the magnitude of theincrease varies from study to study, partly as a result of variations in weather and driving conditions,but also because of differences in methods. The goal of the current study is to improve ourunderstanding of the links between weather and travel risk in mid-sized Canadian cities by using astandardized method for analyzing data from six cities with different climates: Halifax-Dartmouth,Ottawa, Québec, Hamilton, Waterloo Region, and Regina. The study has four interrelated objectives: (1) Toconduct a sensitivity analysis to determine the extent to which risk estimates vary depending onthe criteria used to define precipitation events and `normal' conditions; (2) To compare therelative risk of collision and injury during precipitation relative to `normal' conditions; (3) Todetermine the extent to which weather-related risks vary fordifferent Canadian cities; and (4) To explore any differences in collision characteristics between events and controls, especially as theyvary from city to city. Results are based on a matched-pair analysis, using six-hour time blocks over afour-year period, 1995 to 1998. Results indicate only modest sensitivity to the criteria used to define precipitation events and `normal' conditions. On average, precipitation is associated with a 75 percentincrease in traffic collisions and a 45 percent increase in related injuries, as compared to `normal'seasonal conditions, but risk levels vary depending on the characteristics of the weather event.Both sensitivity to specific weather conditions and weather-related accident profiles vary from city tocity in ways that are not easily explained. 相似文献
6.
三维旋转式自动进样器的研制 总被引:1,自引:0,他引:1
介绍了自行研制的三维旋转式自动进样器的结构和特点,提出了三维模式、气动升降臂、旋转定位的自动进样器解决方案,并成功地用于自动进样器的研制。文章中给出了三维旋转式自动进样器的组成,气动式升降臂控制原理和系统的软件流程。 相似文献
7.
旋挖钻进技术作为一种高效优质的钻进方法近年来广泛地被用于公路、铁路及大型水电站基础工程施工建设中,取得了良好的社会效益和经济效益。探讨了在卵砾石、漂石等复杂地层以及坚硬基岩中进行旋挖钻进所用钻头的结构及其施工工艺。 相似文献
8.
重点介绍了福建马坑矿区ZK8321分支孔受控定向钻进技术。施工中运用了国内先进的螺杆钻造斜技术及光纤陀螺测量定向技术,避开上部复杂孔段(松散破碎层、溶洞地层、采空区等)及对勘探工作的影响,达到了施工目的;总结了施工技术难点与措施,分析了施工技术效果,积累了宝贵的施工经验。 相似文献
9.
This study has evaluated the vertical bearing capacity by conducting static load tests for noise-free and vibration-free screw pretensioned spun high-strength concrete (PHC) piles installed using two different methods (end-squirting shoe and pre-boring methods). Vertical bearing capacity differences seem to occur due to the displacement of soils near the external circumference of a pile, depending on the installation method. A method by which to evaluate the bearing capacity of screw concrete piles is suggested by considering the equations that already have been used to calculate the bearing capacity of piles. Based on static load tests and analysis, the pile installed using the end-squirting shoe method was assumed to be a bored pile and it was reasonable to use the equation proposed by the Japanese Geotechnical Society. At the same time, the pile installed using the pre-boring method was deemed a low soil displacement pile and so it was reasonable to apply the equations proposed for calculating the bearing capacity of the driven pile suggested by the Architectural Institute of Japan. 相似文献
10.
The pile-driving method produces considerable noise and vibrations. Hence, an auger-drilled pile method was developed as a low-noise and -vibration substitute. However, this method does not guarantee the bearing capacity of the pile unless some amount of pile-driving is performed. Therefore, the noise and vibration problems cannot be completely solved. In this study, a prebored screw pile method is proposed to solve these problems. In this method, piles are constructed by the rotary penetration of a screw pile into a prebored hole filled with some cement milk and whose diameter is smaller than that of the screw pile. To determine the shape of the screw pile, laboratory tests with model screw piles were conducted. Also, field load tests were conducted on an actual screw pile fabricated based on the laboratory test result and on a smooth-surfaced pile. In addition, the behavior of the screw pile was estimated by using three-dimensional finite element analysis. The results of the field load test and the numerical simulation showed that the ultimate bearing capacity and the unit skin friction of the screw pile are very superior to those of the smooth-surfaced pile and the cement milk is an important factor in the prebored screw pile method. 相似文献