Direct mid-IR images of Gl 2591,W51-IRS2 and S140

We present the results of direct mid-IR images of the luminous young stellar objects GL 2591, S140-IRS1, and W51-IRS2. The sources show an extended mid-IR emission at the limit of our spatial resolution indicating the presence of dense circumstellar dust disks.     

大黄鱼倍性检测研究

于2001—2004年对人工诱导大黄鱼的不同发育阶段和不同组织进行倍性检测，胚胎眼泡期采用染色体计数法测定，鱼苗和成鱼阶段用流式细胞法，结果表明，胚胎染色体数目2n=48条为二倍体，3n=72条为三倍体．采用流式细胞法测定鱼苗和成鱼个体时，若受测样品的DNA相对含量值与已知对照组二倍体的比值约为1．5：1，其个体为三倍体；比值约为1：1，则为二倍体，无论用肌肉或尾鳍组织，皆可检测出其个体的倍性。     

藻类分子生物技术两年评─—与藻类活性物质研究有关的生物技术

根据１９９２年６月－１９９４年６月国内外发表资料，评述海洋藻类活性物质研究的生物技术及最新成果。认为，海洋生物活性物质研究的重心正转向与海洋生物技术有关的研究。运用生物技术筛选、提取并生产藻类活性物质已经成为这一领域的主流。综合文献表明，藻类活性物质的生物技术正走向成熟，大量的活性物质被发现，光生物反应器将成为主要的下游生产技术，通过藻类基因工程生产药物已初现端倪。     

中国沿海亚历山大藻(Alexandrium)核糖体rDNA部分序列分析及该藻属分子系统进化研究

分析了几株自南海及东海分离的亚历山大藻的rDNA部分序列信息,其中包括核糖体大亚基(LSU)rDNA的5′端D1-D2区序列,以及5.8SrDNA和ITS区序列;同时也对实验室保种的部分来自其它国家和地区的亚历山大藻相关序列进行了测序和分析,并以此作为序列分析中的参考。采用ClustalX及MEGA2软件对所得到的序列信息进行了综合分析与对比。结果表明,分离自南海的塔玛亚历山大藻(Alexandriumtamarense)和分离自东海的链状亚历山大藻(A.catenella),即便是在ITS区和LSUrDNA等高变区,其序列信息也完全一致。与基因库中搜索到的其它亚历山大藻rDNA序列信息相比较,中国沿海的塔玛/链状亚历山大藻序列更接近于塔玛复合种的“亚洲温带”基因型。对于分离自南海的另外两株未定种的亚历山大藻,通过对比序列信息,发现它们与相关亚历山大藻(A.affine)非常接近。分离于我国台湾地区的微小亚历山大藻(A.minutum)在序列上与分离自新西兰的藻株相似,而与分离自欧洲的微小亚历山大藻藻株相差较大。中国沿海亚历山大藻rDNA序列信息的获得为针对有毒藻种设计特异性核酸探针,发展灵敏快速的生物检测技术奠定了基础。     

藻类分子生物技术两年评─—与藻类活性物质研究有关的生物技术

根据1992年6月—1994年6月国内外发表资料，评述海洋藻类活性物质研究的生物技术及最新成果。认为，海洋生物活性物质研究的重心正转向与海洋生物技术有关的研究。运用生物技术筛选、提取并生产藻类活性物质已经成为这一领域的主流。综合文献表明，藻类活性物质研究的生物技术正走向成熟，大量的活性物质被发现，光生物反应器将成为主要的下游生产技术，通过藻类基因工程生产药物已初现端倪。     

从第九届国际光合作用大会看藻类分子遗传学发展趋势

根据１９９２年８月３０日－９月４日在日本名古层如开的第九届国际光合作用大会上交流的以藻类为材料的研究论文，综述了分子遗传学方面的研究成果。认为这些成果体现了光合作用研究的最新进展，蕴含有一定的应用前景；表明类分子遗传学不仅是藻类学研究领域的带头学科，而且已经活跃在光合作用等重大生物学问题研究的前沿；反映了藻类分子遗传学向解决生物学重大问题和推动藻类资料开发方向发展的趋势。     

Morphological variation and reproductive biology of Polaskia chende (Cactaceae) under domestication in Central Mexico

The columnar cactus Polaskia chende, endemic to Central Mexico, occurs in the wild and in silviculturally managed populations where artificial selection for better edible fruits operates by sparing and favouring desirable phenotypes during vegetation clearance. Wild and managed populations were compared in morphology and reproductive biology to analyse if human selection has influenced phenotypic divergence, if it has modified breeding systems and if reproductive isolation exists between them. Fruits from managed populations were larger (6·36±0·17 cm³), heavier (19·62±1·32), sweeter (9·07±0·25 °Brix), and with thinner peel (2·09±0·13 mm) than those from wild populations (5·77±0·18 cm³, 15·73±0·85 g, 8·23±0·31 °Brix, and 2·58±0·14 mm, respectively). Both population types bloom synchronically, show diurnal anthesis, with bees as the most likely pollinators, and have breeding system predominantly self-incompatible. Spatial and temporal barriers to pollen exchange between wild and managed populations are unlikely, and morphological differences would be related with an ongoing process of artificial selection.     

贵州织金新华含稀土磷矿床稀土元素地球化学及生物成矿基本特征

对贵州织金新华含稀土磷矿床进行的稀土元素及微量元素分析结果表明，磷块岩中普遍富集稀土元素，稀土总量∑REE较高，并富集Y元素及La，Nd等轻稀土元素。LREE／HREE比值较高。含稀土磷块岩普遍具Ce负异常，显示其对源区的继承性。指示成磷环境处于氧化程度相对较高状态。稀土元素球粒陨石标准化模式曲线、北美页岩标准化模式曲线、Ce元素异常、LREE／HREE比值、微量元素特征及岩石矿物特征表明，织金新华含稀土磷矿床具以正常海相生物-化学沉积等为主伴有海相热水沉积混合成因的特征。在磷矿成矿过程中沉积物沉积时，活体生物及死亡残骸不同程度地摄取和富集了La，Nd，Ce等稀土元素、Y元素及其他微量元素，沉积在含磷层位，构成大量的含稀土、含生物碎屑白云质磷块岩。     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.