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1.
Cross-flow ultrafiltration (CFF) is often used to obtain separation and concentration of colloids from bulk natural water samples. Application of the ultrafiltration permeation model allows the quantitative determination of the low molecular weight material (LMW, < 1 kDa) and colloids in bulk dissolved organic matter (DOM) from measurements of time series permeate samples obtained from CFF. Detailed analysis of a Yukon River water sample shows that DOM absorption coefficient and fluorescence follow the permeation model and that the complex spectral optical properties of LMW DOM can be reconstructed from CFF data. A combination of measured and modeled data indicates that the LMW contribution to bulk DOM optical properties obtained from CFF can be grossly underestimated by the use of a low concentration factor (CF, the ratio of initial sample volume to retentate volume). Even at a relatively high CF of 19, optical properties of LMW DOM calculated from measurements of the retentate or integrated permeate would underestimate true values by 5–36%. In the Yukon River sample, LMW dissolved organic carbon represented 26% of the bulk concentration, but only 3–14% of the colored DOM was in the LMW fraction while 31–33% of bulk DOM florescence was due to LMW DOM. The contrasting optical properties of LMW and colloidal DOM support the concept that analysis of bulk DOM absorption and fluorescence properties reveals information about DOM molecular weight.  相似文献   
2.
Application of Atomic Force Microscopy to Observation of Marine Bacteria   总被引:3,自引:0,他引:3  
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   
3.
The conditional acid dissociation constants (pKa′) of two sulfonephthalein dyes, thymol blue (TB) and m-cresol purple (mCP), were assessed throughout the estuarine salinity range (0<S<40) using a tris/tris–HCl buffer and spectrophotometric measurement. The salinity dependence of the pKa′ of both dyes was fitted to the equations (25 °C, total proton pH scale, mol kg soln−1):
The estimated accuracy of pH measurements using these calculated pKa′ values is considered to be comparable to that possible with careful use of a glass electrode (±0.01 pH unit) but spectrophotometric measurements in an estuary have the significant advantage that it is not necessary to calibrate an electrode at different salinities. pH was measured in an estuary over a tidal cycle with a precision of ±0.0005 pH unit at high (S>30) salinity, and ±0.002 pH unit at low (S<5) salinity. The pH increased rapidly in the lower salinity ranges (0<S<15) but less rapidly at higher salinities.  相似文献   
4.
Desertification is a severe stage of land degradation, manifested by “desert-like” conditions in dryland areas. Climatic conditions together with geomorphologic processes help to mould desert-like soil surface features in arid zones. The identification of these soil features serves as a useful input for understanding the desertification process and land degradation as a whole. In the present study, imaging spectrometer data were used to detect and map desert-like surface features. Absorption feature parameters in the spectral region between 0.4 and 2.5 μm wavelengths were analysed and correlated with soil properties, such as soil colour, soil salinity, gypsum content, etc. Soil groupings were made based on their similarities and their spectral reflectance curves were studied. Distinct differences in the reflectance curves throughout the spectrum were exhibited between groups. Although the samples belonging to the same group shared common properties, the curves still showed differences within the same group.Characteristic reflectance curves of soil surface features were derived from spectral measurements both in the field and in the laboratory, and mean reflectance values derived from image pixels representing known features. Linear unmixing and spectral angle matching techniques were applied to assess their suitability in mapping surface features for land degradation studies. The study showed that linear unmixing provided more realistic results for mapping “desert-like” surface features than the spectral angle matching technique.  相似文献   
5.
关于绝对重力仪的发展和我们的思考   总被引:1,自引:3,他引:1  
介绍了近年来出现的新型绝对重力仪,认为该仪器是控制地球重力场信息的重要工具,特别对FG5型的如何改进及我国绝对重力仪的如何发展,提出了建议。  相似文献   
6.
岩矿石吸收系数是钻孔电磁波法解决地质问题的重要的物性参数。岩矿石有无吸收系数的差异,是开展钻孔电磁波法能否奏效的前提.了解岩矿石的吸收系数可对钻孔电磁波法的透距和分辨力作出估计,并可为钻孔电磁波的反演解释提供重要的依据.本文不但给出了岩矿石吸收系数的计算方法,也给出了室内岩矿石标本吸收系数的测定方法和野外实测岩矿石吸收系数的方法。  相似文献   
7.
The annual and semi-annual variations of the ionosphere are investigated in the present paper by using the daytime F2 layer peak electron concentration (NmF2) observed at a global ionosonde network with 104 stations. The main features are outlined as follows. (1) The annual variations are most pronounced at magnetic latitudes of 40–60° in both hemispheres, and usually manifest as winter anomalies; Below magnetic latitude of 40° as well as in the tropical region they are much weaker and winter anomalies that are not obvious. (2) The semi-annual variations, which are usually peak in March or April in most regions, are generally weak in the near-pole regions and strong in the far-pole regions of both hemispheres. (3) Compared with their annual components, the semi-annual variations in the tropical region are more significant.In order to explain the above results, we particularly analyze the global atomic/molecular ratio of [O/N2] at the F2 layer peak height by the MSIS90 model. The results show that the annual variation of [O/N2] is closely related with that of NmF2 prevailing in mid-latitudes and [O/N2] annual variation usually may lead to the winter anomalies of NmF2 occurring in the near-pole region. Moreover, NmF2 semi-annual variations appearing in the tropical region also have a close relationship with the variation of [O/N2]. On the other hand, the semi-annual variations of NmF2 in the far-pole region cannot be simply explained by that of [O/N2], but the variation of the solar zenith angle may also have a significant contribution.  相似文献   
8.
石墨炉原子吸收分光光度法测定土壤样品中痕量镉   总被引:2,自引:0,他引:2  
董丽  沈谷苗 《江苏地质》2004,28(1):42-44
研究了土壤样品中痕量镉的石墨炉原子吸收分光光度法测定最佳条件以及磷酸二氢铵与硝酸镁作基体改进剂对测定镉的基体改进效应,并比较了两种试样分解体系。方法检出限为0.01pg/L,精密度(RSD,n=8)为3.29%-7.69%,经国家一级标准物质分析验证,结果与标准值符合。  相似文献   
9.
正20140786Deng Zhenping(Institute of Karst Geology,Chinese Academy of Geological Sciences,Guilin 541004,China);Yang Wen-qiong Application of Stripping Voltammetry with a Solid Amalgam Electrode for Determination of Copper in a Tracer and Groundwater Tracing Experiment(Rock and Mineral Analy-  相似文献   
10.
正20142093Chen Daohua(Guangzhou Marine Geological Survey,Guangzhou 510075,China);Diao Shaobo The Latest Progress of Geological Marine Testing Technology in China(Rock and Mineral Analysis,ISSN0254-5357,CN11-2131/TD,32(6),2013,p.850-859,105refs.)Key words:chemical analysis,China  相似文献   
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