全文获取类型
收费全文 | 477篇 |
免费 | 41篇 |
国内免费 | 107篇 |
专业分类
地球物理 | 34篇 |
地质学 | 195篇 |
海洋学 | 286篇 |
综合类 | 72篇 |
自然地理 | 38篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 6篇 |
2021年 | 6篇 |
2020年 | 9篇 |
2019年 | 7篇 |
2018年 | 9篇 |
2017年 | 9篇 |
2016年 | 18篇 |
2015年 | 9篇 |
2014年 | 19篇 |
2013年 | 18篇 |
2012年 | 19篇 |
2011年 | 24篇 |
2010年 | 21篇 |
2009年 | 27篇 |
2008年 | 30篇 |
2007年 | 38篇 |
2006年 | 30篇 |
2005年 | 33篇 |
2004年 | 20篇 |
2003年 | 19篇 |
2002年 | 31篇 |
2001年 | 19篇 |
2000年 | 20篇 |
1999年 | 12篇 |
1998年 | 22篇 |
1997年 | 16篇 |
1996年 | 18篇 |
1995年 | 19篇 |
1994年 | 18篇 |
1993年 | 9篇 |
1992年 | 16篇 |
1991年 | 11篇 |
1990年 | 7篇 |
1989年 | 2篇 |
1988年 | 6篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 6篇 |
1981年 | 4篇 |
排序方式: 共有625条查询结果,搜索用时 15 毫秒
1.
Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress 总被引:4,自引:0,他引:4
Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature. 相似文献
2.
HUANGGuoqiang DONGShuanglin WANGFang 《中国海洋大学学报(英文版)》2005,4(1):56-60
Attractabilities of different diets and dietary selectivity of Chinese shrimp, Fenneropenaeus chinensis were studied through behavior observation and feeding experiment, respectively. The five diets used in the experiment are: Fish Flesh (FF), Shrimp Flesh (SF), Clam Foot (CF), Polychaete Worm (PW), and Formulated Diet (FD). No significant differences of attractability exist between any two different diets when every two natural diets or all five diets are provided simultaneously. On the other hand, significant differences of attractability exist between FD and every single natural diet when they are provided simultaneously. Results of behavioral observation indicate that natural diets are more attractive than FD. In feeding experiment, Chinese shrimp has distinct selectivity on different diets. It positively selects CF and PW, negatively selects FF and SF, and excludes FD absolutely. The results of the present studies indicate that the dietary selectivity of shrimp was based not only on the attractabilities of the diets, but also on the responses such as growth and food conversion. 相似文献
3.
藏北南羌塘盆地毕洛错地区下侏罗统曲色组石膏岩层 总被引:1,自引:0,他引:1
对西藏自治区北部南羌塘盆地毕洛错地区下侏罗统曲色组石膏岩层进行了研究。根据岩石地层和生物地层资料,确认毕洛错地区的石膏岩层和油页岩为曲色组的一部分,地质时代为早侏罗世,并确认下侏罗统曲色组在区域上可能是寻找油气、油页岩资源的重要层位之一。 相似文献
4.
对虾养殖生态系中有机碳的初步研究本研究由 总被引:1,自引:0,他引:1
对于围隔养殖对虾生态系中有机碳的变动进行了研究 ,结果表明 :溶解有机碳 (DOC)含量波动在 5 .2 99~ 13.39mg/ L之间 ,平均为 8.5 3mg/ L± 2 .2 5 mg/ L;颗粒有机碳 (POC)含量波动在0 .6 5~ 6 .6 3mg/ L之间 ,平均为 3.2 5 mg/ L± 1.76 mg/ L;总有机碳 (TOC)含量波动在 6 .92~ 2 0 .0 2mg/ L之间 ,平均为 11.78mg/ L± 3.82 mg/ L ;其中 DOC∶ POC∶ TOC为 0 .72∶ 0 .2 8∶ 1。各种有机碳组分的含量明显高于自然海水中各种有机碳的含量 ;各种有机碳组分的含量在养殖期间总体上呈上升的趋势 ;其变化与 DCOD的变化呈显著线性正相关的关系。 相似文献
5.
6.
1990年7~8月,舟山地区的对虾养殖场暴发了流行性对虾黑鳃褐斑综合症,造成对虾大量死亡。作者根据这一病情,对病虾的主要器官组织进行了电镜观察。结果发现,病虾鳃丝细胞中有支原体感染。寄生部位为鳃呼吸上皮细胞的细胞质和核周腔。支原体多形态,呈近圆形、细长条形不等,无细胞壁,仅有单一膜包围,中心为一电子密度较低的区域。 相似文献
7.
对虾一种无包涵体杆状病毒病原的PCR检测 总被引:4,自引:0,他引:4
为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义. 相似文献
8.
东海虾类的生态群落与区系特征 总被引:6,自引:0,他引:6
有关东海的虾类,刘瑞玉(1959,1963,1964)、董聿茂(1959,1980,1986)等已做过大量的调查研究。近10多年来,由于东海传统的主要经济鱼类资源衰退,捕食虾类的鱼类减少,因此,使虾类生存空间扩大,这有利于虾类资源的繁衍生长,使虾类资源发生量增多,数量增长较快。东海区三省一市近几年虾类产量达到80×104~90×104t,其中浙江省为60×104~70×104t,拖虾作业已成为东海区一大作业方式,对促进海洋捕捞业的发展起重要作用。20世纪80年代中后期,我们开展了东海虾类资源开发调查,尔后又继续与拖虾生产船相结合进行监测调查,积累了一些资料,本文就东海虾类生态群落结构与区系特征进行探讨。 相似文献
9.
Florence Geret Angela Serafim Luisa Barreira Maria Joo Bebianno 《Marine environmental research》2002,54(3-5)
Copper (Cu) is an essential element for biological systems, however, when present in excess, is toxic. Metallothioneins can play an important role in Cu homeostasis and detoxification. Moreover, Cu can catalyse the production of toxic hydroxyl radicals that cause lipid peroxidation but defence systems in the cells can limit the oxidative damage. The present study was performed to investigate the effect of three Cu concentrations (0.5, 2.5 and 25 μg l−1) on the response of antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), selenium-dependent glutathion peroxidase and total glutathion peroxidase), total proteins, metallothioneins (MT), malondialdehyde (MDA) concentrations in the gills of the clam, Ruditapes decussatus. The activity of antioxidant enzymes and total proteins, MT and MDA concentrations were measured in the gills of the clams after 1, 3, 7, 14, 21 and 28 days of Cu exposure. Results indicate that Cu only induces an imbalance in the oxygen metabolism during the first week of Cu exposure due to a decrease in mitochondrial SOD and CAT, selenium-dependent and total glutathion peroxidase activities. Cu also causes lipid peroxidation, measured by the MDA formation, that was Cu dependent. In the gills of clams exposed to 25 μg Cu/l, the excess of Cu triggers the induction of MT synthesis after 3 days of exposure. 相似文献
10.
Timothy M. Dellapenna Mead A. Allison Gary A. Gill Ronald D. Lehman Kent W. Warnken 《Estuarine, Coastal and Shelf Science》2006,69(3-4):519
To address the relative importance of shrimp trawling on seabed resuspension and bottom characteristics in shallow estuaries, a series of disturbance and monitoring experiments were conducted at a bay bottom mud site (2.5 m depth) in Galveston Bay, Texas in July 1998 and May 1999. Based on pre- and post-trawl sediment profiles of 7Be; pore water dissolved oxygen and sulfide concentration; and bulk sediment properties, it was estimated that the trawl rig, including the net, trawl doors, and “tickler chain,” excavate the seabed to a maximum depth of approximately 1.5 cm, with most areas displaying considerably less disturbance. Water column profile data in the turbid plume left by the trawl in these underconsolidated muds (85–90% porosity; <0.25 kPa undrained shear strength) demonstrate that suspended sediment inventories of up to 85–90 mg/cm2 are produced immediately behind the trawl net; an order of magnitude higher than pre-trawl inventories and comparable to those observed during a 9–10 m/s wind event at the study site. Plume settling and dispersion caused suspended sediment inventories to return to pre-trawl values about 14 min after trawl passage in two separate experiments, indicating particles re-settle primarily as flocs before they can be widely dispersed by local currents. As a result of the passage of the trawl rig across the seabed, shear strength of the sediment surface showed no significant increase, suggesting that bed armoring is not taking place and the trawled areas will not show an increase in critical shear stress. 相似文献