Levels and patterns of genetic variation in Pampus minor:Assessment of a mitochondrial DNA control region sequence

Pampus minor is an important commercial fish. Due to the similarity of external morphological characteristics among the genus Pampus species, P. minor has often been identified as the juvenile group of both P. cinereus and P. argenteus. While little genetic background on this species is known, this study was based on control region sequences and provided the first evaluation of the genetic signature of 264 individuals of P. minor from 11 populations along the coasts of China and Malaysia. The results indicate high genetic haplotype diversity and low nucleotide diversity in this species. Additionally, two differentiated haplotype lineages were identified in the P.minor populations. However, phylogenetic structures corresponding to the geographical locations were unable to be established. Analysis of molecular variance identified a vast majority of the genetic variation occurring within populations. F-statistic test value(FST) of pairwise indicated that great differences existed between the Chinese and Malaysian P. minor populations. For the Chinese populations, the genetic differences were insignificant with the exception of the Xiamen population, which is a marginal population. During the late Pleistocene, a population expansion of P. minor occurred. These expanded populations originated from the glacial refugium in the South China Sea and then rapidly occupied and adapted to their new habitat. The results of this study provide genetic information for ensuring the protection and management of P. minor resources.     

海洋鱼类分子系统地理学研究进展

简要叙述了分子系统地理学的发展简史、研究内容,重点介绍了Avise的5种物种分布模式及中性溯祖理论。并回顾了近年来分子系统地理学的研究进展及其在海洋鱼类研究中的应用,最后对海洋鱼类分子系统地理学研究进行了展望。     

Phylogeographical patterns of the gastropod Siphonaria pectinata (Linnaeus, 1758)

Siphonaria pectinata is a marine gastropod distributed across the shores of the Western and Eastern Atlantic. Although previous studies have indicated that the species could be a recent introduction to Central and North America from Western Africa and Europe, other authors have questioned this statement. In fact, a recent study has shown that individuals from both sides of the Atlantic are genetically disconnected, rejecting the idea of a recent invasion. However, genetic information on this species is very scarce and based only on a single study using a limited number of specimens and sites. The data reported in the present study represent one step forward, collecting information from 119 specimens from 22 sites that cover the entire distributional range of the species. Populations from the Western Atlantic separated from eastern ones around 1.4?1.9 Mya, whereas the centre of dispersion is located on the eastern side of the Atlantic. Siphonaria pectinata should be considered native to the Western Atlantic and cannot be considered as a species with an amphi‐atlantic distribution owing to the lack of genetic connection between both sides of the Atlantic, as previous authors have pointed out. The presence of a star‐shaped phylogeny is discussed in the context of Plio‐Pleistocene glaciations.     

Phylogeographic patterns in Maguimithrax spinosissimus (Decapoda: Mithracidae) from Colombian Caribbean

The Caribbean king crab Maguimithrax spinosissimus is an important artisanal fisheries resource with a distribution range that includes the Florida Keys and Caribbean Sea islands. We carried out a phylogeographic analysis of M. spinosissimus in one oceanic (Old Providence) and two continental islands (Rosario and Tintipán). We analysed 89 and 49 Control Region (CR) and Cytochrome Oxidase I (COI) sequences, respectively. We found genetic differentiation between Old Providence and Rosario?+?Tintipán units (F_ST values of 0.84 for CR and 0.91 for COI gene), and gene flow between Rosario and Tintipán (F_ST values ?0.0085 for CR, P?>?.88; ?0.01 for COI gene, P?>?.99). Our analysis showed two genetic stocks and possibly an isolated biogeographic unit of M. spinosissimus. We suggest managing the species as different populations, and conducting more ecological and biological studies for the determination of possible cryptic species.     

The population genetic structure of the waratah anemone (Actinia tenebrosa) around New Zealand

The genetic structure of populations is often shaped by the reproductive system and larval properties of the species. The waratah anemone (Actinia tenebrosa) reproduces through both asexual clones, which have very short-distance dispersal, and sexual larvae, which are believed to disperse much greater distances. The impact of this mixed strategy on the New Zealand population structure of Actinia tenebrosa was investigated using microsatellite markers. The analysis incorporated 24 sampling locations from around New Zealand and one Australian location, using four microsatellite markers, n = 420. We observed low connectivity and high genetic differentiation between all locations sampled, with a distinct pattern of isolation by distance. The most distinct grouping of locations sampled was the north-east of the North Island from Cape Reinga to East Cape, which was identified by SAMOVA and STRUCTURE analyses as being moderately diverged from the remainder of the country. A tentative correlation was observed between genetic clustering and biogeographic regionalisation, where the distribution of a number of genetic clusters matched previously defined biogeographic regions. Within each location sampled, large numbers of clones were present and a latitudinal cline was observed in the relative contribution of asexually and sexually generated recruits, with an increase in asexual recruits on the South Island's east coast.     

Visualizing Patterns of Genetic Landscapes and Species Distribution of Taxus wallichiana (Taxaceae), Based on GIS and Ecological Niche Models

The Chinese yew (Taxus wallichiana), which is widely distributed in the Himalayas and in southern China, is now on the edge of extinction. In order to understand the evolutionary processes that control the current diversity within this species at the genetic and ecological levels, its genetic patterns and range dynamics must first be identified and mapped. This knowledge can then be applied in the development of an effective conservation strategy. Based on molecular data obtained from 48 populations of T. wallichiana, we used GIS-based interpolation approach for the explicit visualization of patterns of genetic divergence and diversity, and a number of potential evolutionary hotspots have been specifically identified within the genetic landscape maps. Within the maps of genetic divergence and diversity, five areas of high inter-population genetic divergence and six areas of high intra-population genetic diversity have been highlighted in a number of separate mountain regions, and these evolutionary hotspots should have the priority to be protected. Furthermore, four geographical barriers have been identified: the eastern Himalayas, the Yunnan Plateau, the Hengduan Mountains and the Taiwan Strait. According to ecological niche modeling (ENM), the populations of T. wallichiana within the Sino-Himalayan Forest floristic subkingdom experienced westward expansion from the periods of Last Inter-glacial to Last Glacial Maximum (LGM). Following the LGM, the distribution range overall became reduced and fragmented. These findings challenge the classic mode of contraction-expansion in response to the last glaciation. In conclusion, our findings suggest that the changes in geographical landscapes and climate that occurred during the Quaternary resulted in current genetic landscape patterns.     

Significant genetic differentiation among meroplanktonic barrel jellyfish Rhizostoma pulmo (Cnidaria: Scyphozoa) in the Mediterranean Sea

Molecular data have shown that jellyfishes are more geographically restricted and evolutionarily divergent than previously thought. We examined genetic variation and divergence within the meroplanktonic barrel jellyfish Rhizostoma pulmo in the Mediterranean Sea; specific sampling areas were the northern Adriatic, western Mediterranean and Tunisian coast. A total of 19 sampling sites and 68 sequences of the mtDNA cytochrome c oxidase subunit I (COI) gene were used. Of the 68 COI sequences, 45 were newly collected specimens which originated from nine sampling sites along the Tunisian coast. A total of 24 haplotypes were obtained and the specimens sampled were characterised by relatively high levels of haplotype diversity (h = 0.866) and low levels of nucleotide diversity (π = 0.004). Haplotype network analysis showed the presence of three distinct phylogenetic lineages (populations), with separate geographic ranges in the northern Adriatic, western Mediterranean and Tunisian coast. The observed genetic differentiation between these three lineages was supported by the presence of significant genetic differentiation between the 19 populations (F_ST = 0.757, p < 0.001). The high level of genetic differentiation detected in the barrel jellyfish investigated could be attributed to either intrinsic and/or extrinsic barriers to genetic exchange between different populations that may have adapted to different environmental conditions.     

Contrasting genetic diversity with phenotypic diversity in coloration and size in Xantho poressa (Brachyura: Xanthidae), with new results on its ecology

The ecology of Xantho poressa (Olivi, 1792) (Brachyura) was studied during field trips to the Mediterranean Sea, the Black Sea and the Spanish Atlantic Ocean. Our results reveal that X. poressa lives from the intertidal to the shallow subtidal zone, and inhabits relatively protected rocky shores, often with pebble underground, from juvenile to adult stages. A mark–recapture experiment revealed a high population density in this habitat. All stages, but predominantly juveniles, show a variability of colour patterns, which allow the crabs to blend in with the rocky substratum, thereby hiding from predators as passive defence. Adulthood can be reached with a carapace length smaller than 6 mm. The morphometric analysis of the species revealed allometric growth in carapace shape. Variability in overall size could be observed at different collecting sites. Neither the colour morphs nor the size differences could be attributed to differences of Cytochrome Oxidase subunit I mitochondrial DNA sequences, suggesting that ecological rather than genetic patterns are responsible for the different phenotypes.     

Genetic structure and recent population expansion in the commercially harvested deep-sea decapod,Metanephrops challengeri (Crustacea: Decapoda)

ABSTRACT

Determining the genetic structure and population dynamics of harvested species are key aspects of effective fisheries management. New Zealand scampi (Metanephrops challengeri) is a species of nethropid lobster that supports a commercial fishery on the continental shelf and slope around New Zealand. To determine the genetic structure and historical population demography of New Zealand scampi, we sequenced a 623 base-pair fragment of the mitochondrial gene cytochrome c oxidase subunit one (CO1) from 321 individuals across six widely separated sites off the coast of mainland New Zealand and the subantarctic Auckland Islands, representing much of the species’ distribution. The Auckland Islands population was found to be genetically distinct from all other sampled populations, potentially resulting from an absence of continuous suitable habitat between the central-eastern South Island and the Auckland Islands. An isolation-by-distance pattern of genetic structure was also detected. With the exception of the Mercury Islands, all sampled populations showed signs of recent population expansion, potentially linked to changes in habitat availability during the glacial-interglacial cycles of the Pleistocene. We recommend that the current NZ scampi management areas and harvesting rates for each remain unchanged.