不同氮源对微小亚历山大藻生长和毒素产生的影响

通过尿素、氯化铵、酵母浸出粉和硝酸钠等氮源对微小亚历山大藻(Alexandrium minutum)生长及毒素产生的影响研究,分析了微小亚历山大藻对不同氮源利用状况的差异.结果表明,在氮饥饿条件下,加入硝酸钠和酵母浸出粉能显著促进微小亚历山大藻的生长;高浓度的氯化铵在加入后对微小亚历山大藻有一定的毒性效应,表现为生长停滞,但毒性效应在5 d后消失,并得到与添加硝酸钠及酵母浸出粉相似的增长速率0.21 d-1;添加尿素对微小亚历山大藻的生长没有显著促进作用.在四种氮源中,尿素对微小亚历山大藻毒素产生的刺激作用也最弱,在稳定期每个细胞藻细胞毒素含量维持在6.00~8.00 fmol;添加硝酸钠、氯化铵和酵母浸出粉的藻细胞在稳定期毒素含量分别达到11.85,12.86和14.64 fmol.硝酸钠和氯化铵刺激藻毒素产生的效果比酵母浸出粉更为直接.四种含氮营养盐对微小亚历山大藻毒素组成的影响都很小.     

A spectrophotometric measurement of soil cation exchange capacity based on cobaltihexamine chloride absorbance

Among numerous methods for cation exchange capacity (CEC) determination for soils and sediments, the cobaltihexamine chloride method is frequently used due to its ability to measure CEC at soil pH. After exchange with Co(NH₃)₆³⁺ ions, CEC is estimated via the measurement of the Co remaining in solution. The modified method proposed allows a more rapid determination of CEC based on the measurement of the absorbance at 472 nm of the cobaltihexamine chloride solution before and after exchange. This method has been applied to various soil's horizons from four sites, selected to cover a wide range of CEC and pH values. The model obtained allows one to calculate CEC from absorbance at 472 nm with 95% confidence intervals. As CEC is of relevant meaning in agronomical and environmental purposes, and more recently in ecotoxicological studies, this modified method can be proposed as a rapid test for CEC evaluation.     

Lithium and lithium isotope profiles through the upper oceanic crust: a study of seawater-basalt exchange at ODP Sites 504B and 896A

Ocean Drilling Program (ODP) Hole 504B near the Costa Rica Rift is the deepest hole drilled in the ocean crust, penetrating a volcanic section, a transition zone and a sheeted dike complex. The distribution of Li and its isotopes through this 1.8-km section of oceanic crust reflects the varying conditions of seawater alteration with depth. The upper volcanic rocks, altered at low temperatures, are enriched in Li (5.6-27.3 ppm) and have heavier isotopic compositions (δ⁷Li=6.6-20.8‰) relative to fresh mid-ocean ridge basalt (MORB) due to uptake of seawater Li into alteration clays. The Li content and isotopic compositions of the deeper volcanic rocks are similar to MORB, reflecting restricted seawater circulation in this section. The transition zone is a region of mixing of seawater with upwelling hydrothermal fluids and sulfide mineralization. Li enrichment in this zone is accompanied by relatively light isotopic compositions (−0.8-2.1‰) which signify influence of basalt-derived Li during mineralization and alteration. Li decreases with depth to 0.6 ppm in the sheeted dike complex as a result of increasing hydrothermal extraction in the high-temperature reaction zone. Rocks in the dike complex have variable isotopic values that range from −1.7 to 7.9‰, depending on the extent of hydrothermal recrystallization and off-axis low-temperature alteration. Hydrothermally altered rocks are isotopically light because ⁶Li is preferentially retained in greenschist and amphibolite facies minerals. The δ⁷Li values of the highly altered rocks of the dike complex are complementary to those of high-temperature mid-ocean ridge vent fluids and compatible to equilibrium control by the alteration mineral assemblage. The inventory of Li in basement rocks permits a reevaluation of the role of oceanic crust in the budget of Li in the ocean. On balance, the upper 1.8 km of oceanic crusts remains a sink for oceanic Li. The observations at 504B and an estimated flux from the underlying 0.5 km of gabbro suggest that the global hydrothermal flux is at most 8×10⁹ mol/yr, compatible with geophysical thermal models. This work defines the distribution of Li and its isotopes in the upper ocean crust and provides a basis to interpret the contribution of subducted lithosphere to arc magmas and cycling of crustal material in the deep mantle.     

氯化钠浮选剂概述

综述了各类氯化钠浮选剂对氯化钠的浮选。并对各类浮选剂的浮选性能进行了比较。     

Laser Ablation ICP-MS Analysis of Geological Materials Prepared as Lithium Borate Glasses

A simple, rapid and precise method is described for determining trace elements by laser ablation (LA)-ICP-MS analysis in bulk geological materials that have been prepared as lithium borate glasses following standard procedures for XRF analysis. This approach reliably achieves complete sample digestion and provides for complementary XRF and LA-ICP-MS analysis of a full suite of major and trace elements from a single sample preparation. Highly precise analysis is enabled by rastering an ArF excimer laser (λ= 193nm) across fused samples to deliver a constant sample yield to the mass spectrometer without inter-element fractionation effects during each analysis. Capabilities of the method are demonstrated by determination of twenty five trace elements (Sc, Ti, V, Ga, Rb, Sr, Y, Zr, Nb, Cs, Ba, REE, Hf, Ta, Pb, Th and U) in a diverse range of geological reference materials that includes peridotites, basalts, granites, metamorphic rocks and sediments. More than 90% of determinations are indistinguishable from published reference values at the 95% confidence level. Systematic bias greater than 5% is observed for only a handful of elements (Zr, Nb and U) and may be attributed in part to inaccurate calibration values used for the NIST SRM 612 glass in the case of Zr and Nb. Detection limits for several elements, most notably La, are compromised at ultra-trace levels by impurities in the lithium borate flux but can be corrected for by subtracting appropriate procedural blanks. Reliable Pb analysis has proved problematic due to variable degrees of contamination introduced during sample polishing prior to analysis and from Pt-crucibles previously used to fuse Pb-rich samples. Scope exists for extending the method to include internal standard element/isotope spiking, particularly where integrated XRF analysis is not available to characterise major and trace elements in the fused lithium borate glasses prior to LA-ICP-MS analysis.     

无水氯化镍及芳烃基二膦酸四乙酯的制备

1，4—二溴苯，4，4’—二溴联苯、4，4’—二溴三联和亚磷酸三乙酯在催化剂无氯化镍催化下，干燥氮气保护．反应得到相应的有机二磷酸四乙酯。     

确定干旱—半干旱地区降水入渗补给量的新方法——氯离子示踪法

在干旱－半干旱地区由于入渗水分大部分滞留在包气带中，强烈地蒸发、蒸作用导致包气带中土壤水的氯离子浓度改变。氯离子示踪方法从质量守恒角度，通过比较土壤水分的氯离子浓度和降水输入的氯离子浓度大小，可以定量确定降水入渗量和降水入渗补给的历史变化过程。本文介绍了目前国外应用较普遍的氯离子均衡法和氯离子累积法，并讨论了方法应用时存在的一些问题。     

A Reflection on Mg, Cd, Ca, Li and Si Isotopic Measurements and Related Reference Materials

This contribution aims to report the reflections we had with the scientific community during two international workshops on reference materials for stable isotopes in Davos (2002) and Nice (2003). After evaluating the isotopic homogeneity of some existing reference materials, based on either certificates, literature data or specific inter-laboratory rounds, we confirm these as primary reference materials or propose new ones relative to which stable isotope compositions should be reported. We propose DSM-3 for Mg, NIST SRM 915a for Ca, L-SVEC for Li and NBS28 for Si. Cadmium does not yet have a well identified delta zero material, although three commercial mono-elemental Cd solutions have yielded the same isotopic composition relative to one another. In order to scale the linearity of any mass spectrometer, some secondary reference materials are also proposed: Cambridge-1 solution for Mg, the "Münster-Cd" and JEPPIM Cd solutions for Cd and the "Big Batch" silicate for Si. The team from Nancy propose to prepare a mixed spike solution for Li isotopes. Well-characterised natural samples such as ocean or continental waters, diatoms, sponges, rocks and minerals are needed to validate the entire analytical procedure, particularly to take into account the effect of sample mineralisation and of chemical manipulations for elemental separation prior to analysis.     

High-pressure experimental growth of diamond using C–K2CO3–KCl as an analogue for Cl-bearing carbonate fluid

High-pressure, high-temperature diamond growth experiments have been conducted in the system C–K₂CO₃–KCl at 1050–1420 °C, 7.0–7.7 GPa. KCl is of interest because of the strong effect of halogens on the phase relations of carbonate-rich systems [Geophys. Res. Lett. 30 (2003) 1022] and because of the occurrence of KCl coexisting with alkali silicate–carbonate fluids in natural-coated diamond [Geochim. Cosmochim. Acta 64 (2000) 717]. We have used system C–K₂CO₃–KCl as an analogue for these mantle fluids in diamond growth experiments. The presence of KCl reduces the potassium carbonate liquidus to ≤1000 °C at 7.7 GPa, allowing it to act as a solvent catalyst for diamond growth at temperatures below the continental geotherm. This is a reduction on the minimum diamond growth temperature reported in the alkali-carbonate–C–O–H system [Lithos 60 (2002) 145]. Diamond growth using carbonate solvent catalysts is characterised by a relatively long induction period. However, the addition of KCl also reduced the period for diamond growth in carbonate to 5 min; no such induction period appears to be necessary. It is suggested that KCl destabilises carbonate, allowing greater solubility and diffusion of carbon.