NaCl胁迫下杂交稻农艺性状配合力及遗传力分析

采用4×4不完全双列杂交设计,在NaCl胁迫下对杂交水稻主要农艺性状进行了配合力和遗传力分析.结果表明,在调查的10个性状中有4个性状受NaCl胁迫影响差异较大,母本的千粒重及父本的粒厚一般配力差异显著,杂交组合的株高、粒厚、结实率及千粒重特殊配合力差异极显著,其中杂交组合N28S×中花14具有较高的配合力,受NaCl胁迫影响较低。株高及千粒重的广义遗传力和狭义遗传力均较高,可作为杂交水稻耐盐性的选育依据。     

Post-solidification cooling and the age of Io's lava flows

The modeling of thermal emission from active lava flows must account for the cooling of the lava after solidification. Models of lava cooling applied to data collected by the Galileo spacecraft have, until now, not taken this into consideration. This is a flaw as lava flows on Io are thought to be relatively thin with a range in thickness from ∼1 to 13 m. Once a flow is completely solidified (a rapid process on a geological time scale), the surface cools faster than the surface of a partially molten flow. Cooling via the base of the lava flow is also important and accelerates the solidification of the flow compared to the rate for the ‘semi-infinite’ approximation (which is only valid for very deep lava bodies). We introduce a new model which incorporates the solidification and basal cooling features. This model gives a superior reproduction of the cooling of the 1997 Pillan lava flows on Io observed by the Galileo spacecraft. We also use the new model to determine what observations are necessary to constrain lava emplacement style at Loki Patera. Flows exhibit different cooling profiles from that expected from a lava lake. We model cooling with a finite-element code and make quantitative predictions for the behavior of lava flows and other lava bodies that can be tested against observations both on Io and Earth. For example, a 10-m-thick ultramafic flow, like those emplaced at Pillan Patera in 1997, solidifies in ∼450 days (at which point the surface temperature has cooled to ∼280 K) and takes another 390 days to cool to 249 K. Observations over a sufficient period of time reveal divergent cooling trends for different lava bodies [examples: lava flows and lava lakes have different cooling trends after the flow has solidified (flows cool faster)]. Thin flows solidify and cool faster than flows of greater thickness. The model can therefore be used as a diagnostic tool for constraining possible emplacement mechanisms and compositions of bodies of lava in remote-sensing data.     

Occurrence and evolution of the Xiaotangshan hot spring in Beijing, China

Thermal groundwater occurs in bedrock aquifers consisting of the dolomite of the Wumishan Group of the Jixianin System and the Cambrian carbonate in the Xiaotangshan geothermal field near the northern margin of the North China Plain, China. The hot water in the geothermal field of basin-type discharges partly in the form of the Xiaotangshan hot spring under natural conditions. The hot water has TDS of less than 600 mg/L and is of Na·Ca-HCO₃ type. The geothermal water receives recharge from precipitation in the mountain area with elevation of about 500 m above sea level to the north of the spring. Thermal groundwater flows slowly south and southeast through a deep circulation with a residence time of 224 years estimated with the Ra–Rn method. The Xiaotangshan hot spring dried up in the middle of the 1980s owing to the increasing withdrawal of the hot water in the geothermal field in the past decades. The water level of the geothermal system still falls continually at an annual average rate of about 2 m, although water temperature changes very little, indicating that the recharge of such a geothermal system of basin-type is limited. Over-exploitation has a dramatic impact on the geothermal system, and reduction in exploitation and reinjection are required for the sustainable usage of the hot water.     

条斑紫菜(Porphyra Yezoensis Ueda)和孔石莼(Ulve Pertusa Kjellm)对酸耐受力的研究——Ⅰ.pH对条斑紫菜和孔石莼呼吸强度和致死酸度试验

不同pH海水和不同时间处理紫菜、石莼对呼吸强度和致死酸度试验结果表明,紫菜比石莼耐酸力强。在海水温度为18℃条件下,分别用pH_2、pH_3、pH_4、pH_5、pH_6、pH_7、pH_8处理,处理时间20分钟,紫菜除pH_2处理对呼吸强度有抑制作用和培养5天后有20％死亡外,pH_3、pH_4、pH_5、pH_6、pH_7、pH_8各组处理对呼吸强度都有促进作用,培养5天后没有死亡现象,而石莼经pH_2、pH_3处理,对呼吸强度都有抑制作用,培养5天后,pH_2处理组全部死亡,pH_3处理有50％死亡。pH_3和pH_4在海水温度为18℃条件下,处理时间为40分钟时,对紫菜呼吸强度有促进作用,培养5天报,pH_3处理组仅有20％死亡。而pH_3对石莼呼吸强度有抑制作用,培养5天后,全部灭亡。在生产上是否可以考虑用pH_3的海水处理紫菜网帘以达到消除石莼(杂藻)的目的,将是一项具有生产意义的待试验课题。     

假单胞菌(Pseudomonas sp.cn4902)磷酸甘油磷酸酯酶基因克隆与鉴定

采用随机克隆、功能筛选、逐次排除和同源比较的基因克隆新策略进行克隆假单胞菌(Pseudomonas sp．cn4902)磷酸甘油磷酸酯酶基因的研究。结果表明，该结构基因长819bp，与铜绿假单胞菌的磷酸甘油磷酸酯酶基因的核苷酸一致性达61．5％，氨基酸同源性为56．2％。该基因已输入GenBank数据库，收录号AF348165。将该基因转化大肠杆菌，受体菌在含NaCl 1．0mol／L的培养基中甘油含量升高2．9倍，最终菌浓度提高3．6倍。可见这是一个与生物耐盐性相关的主基因，以其转化、培育耐盐农作物的前景十分光明。     

一类典型模糊阵及其在传递闭包化简中的应用

本文研究模糊矩阵传递闭包的化简问题。通过定义 1类典型阵 ,给出传递闭包能够进一步化简的充要条件 ,为网络最大流分析提供了 1种新的模式。     

6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达

本研究根据6-磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物，以假单胞杆菌(Pseudonmonas XM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析，将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Escherichia coli)JM101后检测其蛋白表达及菌株生长耐盐性。     

Physiological and behavioral responses of Bathynerita naticoidea (Gastropoda: Neritidae) and Methanoaricia dendrobranchiata (Polychaeta: Orbiniidae) to hypersaline conditions at a brine pool cold seep

Bathynerita naticoidea (Gastropoda: Neritidae) and Methanoaricia dendrobranchiata (Polychaeta: Orbiniidae) are two of the most abundant invertebrates associated with cold‐seep mussel beds in the Gulf of Mexico. At the methane seep known as Brine Pool NR‐1 (27 °43.415 N, 91 °16.756 W; 650 m depth), which is surrounded by a broad band of mussels (Bathymodiolus childressi), these species have distinctly different patterns of abundance, with the gastropod being found mostly at the outer edge of the mussel bed (average density in November 2003: 817 individuals·m⁻² in outer zone, 20·m⁻² in inner zone) and the polychaete being found almost exclusively near the inner edge (average density in November 2003: 3155 individuals·m⁻² in inner zone, 0·m⁻² in outer zone), adjacent to the brine pool itself. The salinity of the brine pool exceeds 120, so we hypothesized that M. dendrobranchiata should be more tolerant of high salinities than B. naticoidea. The opposite proved to be true. The gastropods were capable of withstanding salinities at least as high as 85, whereas the polychaetes died at salinities higher than 75. Both species were osmoconformers over the range of salinities (35–75) tested. Behavioral responses of B. naticoidea to salinities of 50, 60, and 70 were investigated in inverted vertical haloclines. Gastropods generally did not enter water of salinity greater than 60, but tolerated short periods at 60. Behavioral avoidance of brine should limit the vertical distribution of B. naticoidea in the inner zone to the top 2.5–5 cm of the mussel bed. Behavior is also a likely (though unproven) mechanism for controlling horizontal distribution of this species across the mussel bed. Methanoaricia dendrobranchiata can tolerate short excursions into the brine, but probably avoids hypersaline conditions by aggregating on the tops of the mussels.     

近海海洋石油平台导管架喷涂金属层防腐

锌、铝及其合金涂层对钢铁的防护，不仅对阴极起保护作用(Gartland，1987)， 对涂层本身也具有良好的抗腐蚀性能。此外，涂层中金属微粒表面形成的致密氧化膜，也起到了防腐蚀的作用。在不同大气环境下，锌、铝有良好的耐蚀性，其腐蚀速率比钢铁要低得多(李言涛，1998)。采用热喷涂锌、铝及其合金涂层对钢铁构件和构筑物进行长效防护早在20世纪20年代就已开始应用，至今仍是普遍采用的防护措施，并在继续发展(李守本等，1989)。20年代初，法国首先用于海水闸门的防腐；40年代美国用于墨西哥湾的海上井架和海上输油管以及舰船的防腐；60年代英、法、德等国海军将这一技术扩大应用到舰船的上部结构和船壳。而金属热喷涂技术在海洋工程中使用非常有限，第一次使用热喷铝涂层防护近海平台的实例是Conoco公司在北海的Murchison结构上的锥形塔，并且已获得了4a良好的使用效能(Fisher et al.，1987；Shaw et al.，1985)。1984年6月，Hutton张力支柱平台(TLP)在北海下水(水深148 m)安装。系链、升降机和锥形塔均采用火焰热喷涂技术喷涂铝涂层进行防护(Tyson，1985)。1992年6月，Hotton平台使用8a后，对升降机绳索进行观察，在飞溅区没有发现腐蚀现象，也没有检测到褐色渗漏效应(Fisher et al.，1995)。