黄斑篮子鱼去毒相关基因的克隆与肝脏组成型表达分析

从基因水平探讨海洋鱼类对海洋藻毒素的去毒分子机理。采用RT-PCR法成功克隆了黄斑篮子鱼Siganus oramin肝脏I时相代谢酶细胞色素P450 1A(CYP1A)、II时相代谢酶alpha型谷胱甘肽S-转移酶(GSTA)和rho型谷胱甘肽S-转移酶(GSTR)、热休克蛋白70 (HSP70)、alpha 1型钠钾ATP酶(ATP1A1)及β-肌动蛋白(beta-actin, ACT)基因cDNA核心序列,序列分别长879 bp、582 bp、588 bp、660 bp、749 bp和554 bp。序列同源性分析发现,属鲈形目的黄斑篮子鱼CYP1A、GSTA和GSTR与同属鲈形目的牙鲆Paralichthys olivaceus、欧洲鲽Pleuronectes platessa、真鲷Pagrus major、鲤形目的斑马鱼Brachydanio rerio 相应氨基酸序列同源性较高,CYP1A和GSTA与非洲爪蟾(两栖类)、鸡(鸟类)、小鼠、大鼠和人(哺乳类)相应氨基酸序列同源性低,这可能与鱼类I、II时相去毒酶基因承担水环境毒素去毒代谢的特殊功能有关;而HSP70、ATP1A和β-肌动蛋白在鱼类、两栖类、鸟类、哺乳类中均有较高的同源性,这可能与这些基因在机体中承担的最基本的生命功能相关。应用半定量RT-PCR的方法,以β-肌动蛋白作为外参照,在指数期增长范围内分别得到了CYP1A、GSTA、GSTR、HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA (%)的比值,确定黄斑篮子鱼肝脏去毒相关基因的组成型表达水平。其中,黄斑篮子鱼肝脏CYP1A、GSTA和GSTR基因组成型表达相对较高,HSP70和ATP1A1基因组成型表达相对较低,这可能与不同基因在黄斑篮子鱼海洋藻毒素去毒分子机理中承担的作用相关,为海洋藻毒素在海洋鱼类中的积聚及代谢去毒分子机制的研究提供了相关数据。     

Chandra observations of neutron stars: an overview

We present an overview of Chandra X-ray Observatory observations of neutron stars. The outstanding spatial and spectral resolution of this great observatory have allowed for observations of unprecedented clarity and accuracy. Many of these observations have provided new insights into neutron star physics. We present an admittedly biased and overly brief review of these observations, highlighting some new discoveries made possible by the Observatory’s unique capabilities. This includes our analysis of recent multiwavelength observations of the putative pulsar and its pulsar-wind nebula in the IC443 SNR.      

P－350浸渍树脂分离富集Ag，Cd体系及其应用

以Ｐ－３５０吸附于ＤＡ＿（２０１）树脂上，制得了Ｐ－３５０浸渍树脂，通过柱层析法，试验了树脂吸附和解脱Ａｇ，Ｃｄ的条件和干扰离子的影响。建立了Ｐ－３５０浸渍树脂分离－富集Ａｇ，Ｃｄ体系，并应用于岩石样品分析中，效果较好。     

A deep search for pulsar wind nebulae using pulsar gating

Using the Australia Telescope Compact Array (ATCA) we have imaged the fields around five promising pulsar candidates to search for radio pulsar wind nebulae (PWNe). We have used the ATCA in its pulsar-gating mode; this enables an image to be formed containing only off-pulse visibilities, thereby dramatically improving the sensitivity to any underlying PWN. Data from the Molonglo Observatory Synthesis Telescope were also used to provide sensitivity on larger spatial scales. This survey found a faint new PWN around PSR B0906−49; here we report on non-detections of PWNe towards PSRs B1046−58, B1055−52, B1610−50 and J1105−6107. Our radio observations of the field around PSR B1055−52 argue against previous claims of an extended X-ray and radio PWN associated with the pulsar. If these pulsars power unseen, compact radio PWNe, upper limits on the radio flux indicate that a fraction of less than 10⁻⁶ of their spin-down energy is used to power this emission. Alternatively, PSRs B1046−58 and B1610−50 may have relativistic winds similar to other young pulsars and the unseen PWN may be resolved and fainter than our surface brightness sensitivity threshold. We can then determine upper limits on the local interstellar medium (ISM) density of 2.2×10⁻³ and 1×10⁻² cm⁻³, respectively. Furthermore, we derive the spatial velocities of these pulsars to be ∼450 km s⁻¹ and thus rule out the association of PSR B1610−50 with supernova remnant (SNR) G332.4+00.1 (Kes 32). Strong limits on the ratio of unpulsed to pulsed emission are also determined for three pulsars.     

近断层竖向地震动P-Δ效应研究

竖向效应是近断层地震动的主要特点,竖向地震动对工程结构形成的P-Δ效应是造成结构破坏的主要原因。本文以单自由度体系为研究对象,选取汶川地震的近断层强震数据进行研究,分析竖向地震动所产生P-Δ效应的放大作用特征。分析结果表明汶川地震中,相比于中远场区域,近断层区域内竖向地震动作用所产生的P-Δ效应具有明显的放大作用,且近断层内这种放大作用具有普遍性;当自振周期一定时,不同台站获得地震动的放大系数离散性较小,主要分布于均值附近;其分析结果符合地震动的一般规律,分析结果合理。在此基础上建立了以均值为代表值的放大系数谱,可以与抗震设计反应谱结合使用,从而为活断层附近区域内的结构抗震设计问题提供定量化参考依据。     

基于动态图像变形的PP与PS波层位直接匹配

多分量地震资料的矢量偏移、多波地震资料的联合解释与反演均需要估算纵横波的速度比,实现纵波与转换横波在时间或深度域的匹配.基于DTW,本文实现了一种适用于PP与PS波直接匹配的动态图像变形算法.该算法分为三个部分:首先,使用二阶对称动态规划算法逐样点递归计算PP与PS波走时或深度的误差累积和;其次,在以误差累积和为目标函数的回溯阶段设定变形窗,并在纵横波速比约束的变形窗内递归回溯搜索匹配路径;最后,根据最大相关系数判定准则在匹配路径中确定最佳匹配路径,获得使PP与PS波匹配的拉伸或压缩时移量.利用所获得的拉伸压缩时移量计算纵横波速度比就可以实现PP与PS波之间的匹配.模型与实际陆上多分量地震资料测试结果表明:该方法具有较高的匹配精度,且对于信噪比、相似度较低的多分量地震资料,该方法也能产生较好的匹配效果.     

南阿尔金巴什瓦克基性麻粒岩的变质演化 P- T- t 轨迹

大陆碰撞造山带中高压麻粒岩的 P- T- t 轨迹研究对于理解造山带的热演化历史及大陆地壳的形成与演化具有重要的意义,然而,如何连接同位素年龄与变质演化过程是恢复和建立 P- T- t 轨迹的重点和难点。本文通过对南阿尔金巴什瓦克地区基性麻粒岩的详细岩相学研究,认为该基性麻粒岩经历了原岩阶段（M1）、峰期变质阶段（M2）、峰后退变质阶段（M3）以及晚期角闪岩相- 绿片岩相退变质阶段（M4）。其中,传统矿物温压计和矿物微量元素温压计获得该基性麻粒岩所记录的峰期变质条件分别为17. 5~22. 6 kbar,901~985℃ 和17~28 kbar,1012~1049℃,退变质阶段的温压条件为7. 6~10. 7 kbar,750~810℃。此外,锆石U- Pb 年代学结果表明基性麻粒岩的变质时代为491±3. 5 Ma (MSWD=0. 62),结合锆石和石榴子石的微量元素分配系数以及前人的实验岩石学数据,认为该变质时代记录了早古生代高压- 超高温的变质事件,进而恢复了南阿尔金基性麻粒岩所记录的顺时针 P- T- t 轨迹。     

The δ60/58Ni Values of Twenty‐Six Selected Geological Reference Materials

The high‐precision δ^60/58Ni values of twenty‐six geological reference materials, including igneous rocks, sedimentary rocks, stream sediments, soils and plants are reported. The δ^60/58Ni values of all samples were determined by double‐spike MC‐ICP‐MS (Nu Plasma III). Isotope standard solution (NIST SRM 986) and geological reference materials (BHVO‐2, BCR‐2, JP‐1, PCC‐1, etc.) were used to evaluate the measurement bias and intermediate precision over a period of six months. Our results show that the intermediate precision of Ni isotope determination was 0.05‰ (2s, n = 69) for spiked NIST SRM 986 and typically 0.06‰ for actual samples, and the δ^60/58Ni _{NIST SRM 986} values were in excellent agreement with previous studies. Eighteen high‐precision Ni isotope ratios of geological reference materials are first reported here, and their δ^60/58Ni values varied from ?0.27‰ to 0.52‰, with a mean of 0.13 ± 0.34‰ (2s, n = 18). Additionally, SGR‐1b (0.56 ± 0.04‰, 2s), GSS‐1 (?0.27 ± 0.06‰, 2s), GSS‐7 (?0.11 ± 0.01‰, 2s), GSD‐10 (0.46 ± 0.06‰, 2s) and GSB‐12 (0.52 ± 0.06‰, 2s) could potentially serve as candidate reference materials for Ni isotope fractionation and comparison of Ni isotopic compositions among different laboratories.     

Double-difference tomography of P- and S-wave velocity structure beneath the western part of Java,Indonesia

West Java in the western part of the Sunda Arc has a relatively high seismicity due to subduction activity and faults. In this study, double-difference tomography was used to obtain the 3D velocity tomograms of P and S waves beneath the western part of Java. To infer the geometry of the structure beneath the study area, precise earthquake hypocenter determination was first performed before tomographic imaging. For this, earthquake waveform data were extracted from the regional Meteorological, Climatological, Geophysical Agency (BMKG) network of Indonesia from South Sumatra to Central Java. The P and S arrival times for about 1,000 events in the period April 2009 to July 2016 were selected, the key features being events of magnitude > 3, azimuthal gap < 210° and number of phases > 8. A nonlinear method using the oct-tree sampling algorithm from the NonLinLoc program was employed to determine the earthquake hypocenters. The hypocenter locations were then relocated using double-difference tomography (tomoDD). A significant reduction of travel-time (root mean square basis) and a better clustering of earthquakes were achieved which correlated well with the geological structure in West Java. Double-difference tomography was found to give a clear velocity structure, especially beneath the volcanic arc area, i.e., under Mt Anak Krakatau, Mt Salak and the mountains complex in the southern part of West Java. Low velocity anomalies for the P and S waves as well as the v_P/v_S ratio below the volcanoes indicated possible partial melting of the upper mantle which ascended from the subducted slab beneath the volcanic arc.