Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.     

贻贝粉酶解液提取复合氨基酸

报道了以脱脂后的贻贝粉为原料，采用酶解法从其酶解液中分离提取复合氨基酸的工艺。其收率为２０％，纯度约为９０％。其中必需氨基酸（不计色氨酸）约占复合氨基酸总量的５５％，其氨基酸模式与ＦＡＯ／ＷＨＯ组织确定的模式及人乳模式进行了对照，通过适量添加个别氨基酸可使其配比接近人乳模式。     

Total and methylmercury in a Brazilian estuary,Rio de Janeiro

Guanabara Bay, in Rio de Janeiro state, is impacted by organic matter, oil and heavy metals. The present study evaluated the total mercury (THg) and methylmercury (MeHg) concentrations and the MeHg to THg ratio (%MeHg) in water samples from different points of the bay and in 245 organisms of three different trophic levels sampled between 1990 and 2000. Dissolved mercury concentration in estuarine water samples ranged from 0.72 to 5.23 ngl(-1). THg and MeHg in mussel, ranging from 11.6 to 53.5 microg THg kg(-1) wet wt. and 4.5-21.0 microg MeHg kg(-1) wet wt., varied according to sampling point and water quality. Planktivorous fish and mussel presented similar MeHg concentrations, meanwhile THg in planktivorous fish were lower than in mussel. Carnivorous fish showed higher THg and MeHg concentrations (199.5 +/- 119.3, 194.7 +/- 112.7 microg kg(-1) wet wt. respectively) than organisms from other feeding habits and lower trophic levels. There was a significant difference in the %MeHg among trophic levels: mussel presented lower MeHg percentage (33%) than planktivorous fish (54%) and carnivorous fish (98%).     

Tourist farms in Lower Silesia, Poland

In view of the small size of most Polish farms, attempts have been made to encourage diversification into rural tourism. The paper examines the progress made in Lower Silesia which has good resources for domestic and international tourism. Thanks to the promotional work of the Agricultural Consultative Centres over a hundred farms are now providing services. They do not generate a large share of the farm income but business is expanding and some areas are becoming very popular. Thus a base has been established for future expansion. Further development depends on easier access to credit and government support for ecological agriculture. Better promotion is also needed to increase interest in rural tourism in Poland and attract more visitors from abroad. This revised version was published online in July 2006 with corrections to the Cover Date.     

Lessons from stakeholder dialogues on marine aquaculture in offshore wind farms: Perceived potentials,constraints and research gaps

Drawing on a case study in Germany, this contribution explores the practical application of offshore aquaculture within offshore wind farms in view of the different stakeholders involved. Using a transdisciplinary research approach, an understanding of the rationalities and interests among the different involved stakeholder groups was explored. Offshore wind energy is high on the political agenda in Germany. The vast spatial requirements however inherit potential user conflicts with competing, and under current legislation excluded users such as fishermen. Solutions for combining sustainable uses of the same ocean space have thus seen increasing interest within the research community in Germany and in Europe over the past years. This paper was inspired by and presents the outcomes of a stakeholder analysis and in particular a stakeholder workshop. Central focus was placed on academics and private as well as public stakeholders engaged in current research efforts of combining offshore wind farms and aquaculture in the German North Sea. The paper identifies the overall acceptance of such a multi-use scenario in society, opportunities and constraints as perceived by the stakeholders, and key research gaps. The results confirm the assumption that there is a clear need, and also willingness on behalf of the policy makers and the research community, to find sustainable, resource- and space-efficient solutions for combined ocean use.     

韩国济州岛柑橘园景观与空间分布演变（1526—2019年）

韩国济州岛的柑橘园景观具有重要的文化遗产价值,是当地人利用济州岛特殊的气候、水文、植被环境所塑造的独特农业景观。16世纪朝鲜王朝建立柑橘进贡制度,济州官府设置柑橘园,此后至今,济州岛柑橘园区域分布与地理景观都呈现较大变迁。文章结合气候、水资源等自然因素与韩国柑橘产业政策、市场、社会变化与技术进步等人文因素,分析16世纪以来济州岛柑橘园景观与空间分布变化,探讨济州岛柑橘生产空间变迁原因与驱动力。结果显示：16世纪至2019年,济州岛柑橘生产经历了发展、没落、强力复兴的大起大落发展历史,呈现从北到南的生产空间大迁移,柑橘生产空间逐渐扩大。济州岛的柑橘园生产经历从朝鲜时期的官府果园到现代观光体验农场的转变。柑橘生产空间变迁与地理景观变化的原因与驱动力较复杂,水资源是影响朝鲜时期柑橘园分布的主要自然因素。社会制度、交通与市场是16世纪以来影响柑橘园生产空间扩展的主要人文因素,在近500年的柑橘生产空间发展变迁的过程中,人文因素不断加强,影响柑橘空间与地理景观的优化调整。20世纪70年代以来,水资源开发技术的进步改变了现代柑橘园生产空间分布格局。对济州岛柑橘园长时段的生产空间与地理景观变化及其作用机制的研究,可以为中国传统柑橘栽培地区打造具有地方特色柑橘园文化景观与民俗、文化产品,以及中国柑橘产业的升级发展提供参考。     

Solid phase characterization and metal deportment in a mussel shell bioreactor for the treatment of AMD,Stockton Coal Mine,New Zealand

The Stockton Coal Mine, located on the West Coast of New Zealand, is evaluating the use of a mussel shell bioreactor (MSB) to treat acidic metalliferous runoff from acid forming overburden. This novel approach is similar in concept to vertical flow wetlands (VFWs) and successive alkalinity producing systems (SAPS). The MSB system is a trapezoidal pit 2 m deep, 35 m long, 3–10 m wide with 60° angle sides. During operation it contained 160 tonnes (240 m³) of mussel shell material and was saturated with a 100–200 mm water cap. Influent flowed through the reactor at a mean rate of 0.3 L s⁻¹ resulting in a hydraulic retention time (HRT) of ≈6 days. The prototype MSB was in operation for a total of 1027 days, from June 2009 through March 2012, and effectively sequestered 99.7% of Al, 99.3% of Fe, 98.8% of Ni, 98.4% Tl and 99.3% of Zn, as determined from a previous evaluation of MSB performance. The MSB also effectively neutralized acidity, which resulted in an increase in influent pH from 2.8 to 6.9 in the effluent. Based on an examination of several excavated pits, five distinct reaction zones developed within the MSB. The reaction zones consisted of an allochthonous sediment layer (0–330 mm), an oxidized iron-rich ocherous layer (at 330–350 mm depth), an aluminum layer (at 350–600 mm depth) with geochemical variations throughout (350–500 mm and 500–600 mm); and a chemically reduced bottom shell layer (at 600–1100 mm). Representative samples were collected from each layer and analyzed using a combination of geochemical and physical methods to assess the stability of the secondary minerals and trace metal deportment within the MSB. Major elements Fe, Al, Ni, Tl, and Zn where preferentially associated with particular layers within the MSB. Elevated concentrations of Fe (110,000 mg kg⁻¹) were observed in the allochthonous sediment and ocherous precipitate layers, while Al (27,816 mg kg⁻¹), Ni (55 mg kg⁻¹), and Zn (655 mg kg⁻¹) were elevated within the aluminum and lower reduced depths within the MSB. Trace Tl (21 mg kg⁻¹) showed varying concentrations throughout the MSB, but was strongly correlated to lower layers of the system. Microbial biofilms were observed within the reduced portions of the shell layers often proximal to bacterial shaped sulfides. The geochemical assessment of the MSB presented in this study is the first of its kind for a MSB, and supports the argument that this system is another viable option for passive treatment of AMD.