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Abbondanzi F Campisi T Focanti M Guerra R Iacondini A 《Marine environmental research》2005,59(5):419-434
A study was conducted to determine polycyclic aromatic hydrocarbons (PAHs) distribution and microbial population changes in brackish sediments from an Italian lagoon included in the Ramsar List of Wetlands of International Importance. The presence and level of PAH-degrading bacteria were estimated by the most probable number (MPN) enumeration technique, whereas degradation capability towards target compounds was checked against loss of spiked PAHs (Phenanthrene, Anthracene and Fluoranthene) in MPN tubes after incubation in optimal conditions. Chemical analyses and microbiological counts suggested a potential for PAHs biodegradation by natural occurring populations of sediment microorganisms, thus indicating an "optimal range" in sediment PAHs concentrations, outside of which the natural selection of the indigenous microflora did not occur. The MPN procedure here described, provided an effective and reliable way to simultaneously determine microbial population densities and subsequent confirmation of the biodegradation capability of sediment indigenous microflora when exposed to laboratory and environmental concentrations of PAHs. 相似文献
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Igor E. Agranovski 《洁净——土壤、空气、水》2007,35(1):111-117
A new personal bioaerosol sampler has been developed and verified as an efficient tool for monitoring of viable/non‐viable airborne microorganisms, including bacteria, fungi, and viruses. The operational principle of the device is based on continuous passage of an air sample through porous media submerged into a liquid layer. During motion along narrow and tortuous ways inside the porous media, the air stream is split into a large number of ultra small bubbles with the particulates are being scavenged by these bubbles and, thus, effectively trapped. The device was initially verified for monitoring of viable airborne bacteria and fungi, firstly, under controlled laboratory conditions and later in a field. It was demonstrated that bacterial recovery rates for these two groups of microorganisms were very high and the device was found to be fully feasible for such monitoring. The next step of the device investigation was performed in the laboratory on monitoring viable airborne viruses with a range of sensitivities to physical and biological stresses. As the result, the new personal sampler demonstrated a very high recovery rate even for viruses which are rather sensitive to environmental stress (Avian Influenza, SARS, Mumps, etc.). Some following field studies, undertook in a hospital and animal houses, also demonstrated an excellent performance of the new device for selective and reliable monitoring of viable airborne viruses even in environments highly contaminated by other microorganisms. This paper reviews the main development staged of the new personal bioaerosol sampler. 相似文献
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微生物对生命元素如碳、氮、硫、氧和金属离子的代谢作用能显著的改变微生物周边的外部环境和其内部环境。在一系列的生物地球化学过程中,微生物参与了矿产的沉积(生物成矿)或参与了矿石和岩石的溶解(生物风化)。生物成矿作用有两个途径:一个叫生物诱导成矿,通过这个过程,微生物分泌出代谢产物导致了之后的矿物颗粒的沉积;另一个叫生物控制成矿,在这个过程中,微生物在控制矿物成核和生长上起到了显著作用。微生物成因的矿物总体来说颗粒都很小和/或有着独特的同位素特征。最普遍的生物成因矿物有碳酸盐、硫化物和铁的氧化物。细胞表面和其分泌的胞外聚合物的结构可以为离子的浓缩、聚合和矿化提供模板,并起到重要作用。地球材料的仿生合成帮助我们了解了在人工条件下的生物成矿机制。此外,在地质环境中生物成因的矿物还可以作为一种生物信号,用来重建地球和其他行星的起源和演化。 相似文献
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A natural decay of plant and animal biomass in aquatic environments results in the release of different simple structured organic substances into water. In our laboratory experiments we investigated whether some of them, e.g., starch or peptone, and also a complex yeast biomass can be transformed into humic-like substances by natural assemblages and some pure cultures of aquatic microorganisms. After a 6 months incubation most of the cultural media enriched with those natural organic substrates turned dark in color and humic-like substances (HS) could be isolated. However, the original substrate organic carbon was mainly mineralized in microbial cultures, and only about 3 % C was converted into HS. Total yields of HS differed in dependence of the individual substrate used (peptone > yeast > starch), the origin of inoculum (river > lake > groundwater), and the incubation temperature (20°C > 10°C). According to their elemental composition, and their spectroscopical and electrophoretic characteristics the microbially formed HS resembled natural aquatic humic matter, but were higher in aliphatic constituents (carbohydrates, peptides) and lower in aromatic structures. HS-like substances were also obtained from pure cultures of a bacterium Bacillus sphaericus and a fungus Cladosporium cladosporioides. The 13C and 15N isotopic contents of the microbially produced HS differed in dependence on the microbial inoculum and the type of organic substrates used, but in general they indicated anabolic processes to be mainly responsible for the humification of the simple organic substrates used in our experiments. 相似文献
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Hidekazu Yoshida Koshi Yamamoto Yuki Murakami Nagayoshi Katsuta Toru Hayashi Takeshi Naganuma 《Environmental Geology》2008,55(6):1363-1374
Takashikozo is a phenomenon of Quaternary sediments in Japan. They are cylindrical Fe-oxyhydroxide nodules that form as plaques round
plant roots, where Fe is preferentially concentrated to develop a solid wall. Structural features suggest that after the roots
have decayed, the central space where the roots were situated acts as a flow path for oxidized water. Analysis of microbial
16S rDNA extracted from the nodules identified iron-oxidizing bacteria encrusted round the roots where they are the likely
initiators of nodule formation. Direct microscopic observation revealed an accumulation of Fe-oxyhydroxides that fill the
pore spaces and is also likely to be linked with the encrusting microbial colonies. Geological history and nanofossil evidence
suggest that these Fe-nodules may have been buried at a depth of up to several tens of meters for at least 105 years in reducing Quaternary sediments. Thus Fe-oxyhydroxide nodules that have formed in a geological environment at the
interfaces between water and rock by microbial mediation can persist under reducing conditions. If this is the case, the phenomenon
is significant as an analogue of post-closure conditions in radioactive waste repositories, since it could influence nuclide
migration. 相似文献
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Discharge areas of hydrothermal springs are known to be inhabited by diverse types of microorganisms including archaea, prokaryotes and eukaryotes. A total of 11 hydrothermal samples from the Rio Grande rift and the Valles caldera in New Mexico were analyzed to investigate the correlation between chemical and microbiological parameters of hydrothermal waters. The sampled fluids are categorized into three chemical groups: (I) steam-condensing acid sulfate waters, (II) deep geothermal and derivative waters and (III) thermal meteoric waters. Analyses of the microbial phospholipid fatty acids and denaturing gradient gel electrophoresis of DNA show that acid sulfate waters were populated by thermoacidophilic organisms and had high biomass content. Mineralized deep geothermal and derivative waters exhibited a high degree of microbial diversity, but had low biomass content. Thermal meteoric waters are low in total dissolved solids, and exhibit very low biomass content and microbial diversity. DNA sequences from several previously unknown microbial species were detected. The results of this study support the hypothesis that microbes can be used as tracers for specific types of subsurface environments. 相似文献
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