南海带鱼遗传变异及其分类的研究

带鱼是我国海洋鱼类中最重要的经济鱼类,过去进行了大量的研究,多认为中国近海的带鱼为一个种,而不同的学者分别认为存在3,4和5个种群。从1976年开始,我们试图主要用生化方法并结合形态学资料研究带鱼的分类。1983和1988年报道了我们通过带鱼肌浆蛋白和形态学部分资料的研究认为中国近海带     

山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析

采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场，各20尾，取全血或肌肉组织，以酚-仿抽提方法提取基因组DNA，利用筛选获得的10对微卫星引物进行PCR扩增，反应产物经8％非变性聚丙烯酰胺凝胶电泳分离、EB显色，用ImageMasterl D Elite(Version 3．01)软件分析电泳结果，并计算了相应的遗传学参数。结果表明，在自然和养殖群体中，10个基因座位的等位基因平均数(α)分别为6．7和6．1，每个基因座位有效等位基因数(αe)分别为1．8—6．8和2．5—6．7，群体平均杂合度(H)分别为0．8120和0．7310；两个群体间的遗传相似性系数、遗传距离和基因分化系数为0．8558、0．1557和0．0558；自然群体内每个座位上的多态信息含量(PIC)为0．59—0．84、个体识别率(DP)为0．54—0．86、非父排除率(PPE)为0．41—0．72，其累积个体识别率和非父排除率均达到0．9999，表明所选座位属中高识别力的遗传标记，可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。     

中华乌塘鳢的群体生物学研究 Ⅱ.生化遗传变异

用同工酶谱法发现中华乌塘鳢Bostrichthy sinensis四个群体的遗传变异水平很低,在所检测的30个基因座位中,多态座位比例只有0—10％,平均杂合度仅为0.2—0.5％,物种的平均杂合度为0.33±0.10％。四个群体共享常见的等位基因。虽然其中三个群体拥有特有的等位基因,但其基因频率都未超过5％。四个群体的Nei氏遗传距只有1.68—12.13×10~(-5)。据分析,近交可能是遗传变异水平低和形态特征分化差的主要原因。种苗生产上应注意广泛采集不同地理群体的鱼作亲鱼,以丰富养殖群体的基因库。     

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification

An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.     

Mooring optimization of floating platforms using a genetic algorithm

This paper presents a new procedure for the optimization of the mooring design of floating platforms, in which an automatic design sequence is also established. Regarding the optimization philosophy, the following aspects are dealt with:

• The optimization of the platform heading and its mooring pattern, taking into account the environmental force spreading;

• optimum line length or line tension for each mooring line, associated to the optimization of the mooring line materials and sizes.

Basically, the main goal of this paper is to introduce a new method, which will provide the quickest way to find the best mooring system, defined here as that which minimizes platform responses.A genetic algorithm (GA) is applied in this contribution, and this paper describes exactly the procedure of developing a GA code directed toward the solution of mooring design optimization problems. In order to prove the efficiency and the vast potential of the proposed algorithm as a design tool, sample moorings are analyzed for different environmental conditions and the final results, including the time required to run them, are presented.     

翘嘴红鲌(Erythroculter ilishaeformis Bleeker)种群遗传多样性的RAPD分析

采用RAPD技术对翘嘴红鲌养殖群体进行了DNA多态性检测.共采用40个随机引物进行扩增,其中14个引物可获得清晰且重复性好的扩增图谱,共扩增出121个RAPD位点,具有多态现象的位点为40个,多态位点比例为33.06%.翘嘴红鲌群体的遗传相似系数为 0.8878-0.9600,平均为0.9201,遗传变异度为0.0799.Shannon多样性指数为10.8824,Shannon多样性值为0.0899.研究表明,翘嘴红鲌目前的种质资源状况令人堪忧,改变目前人工繁育模式,建立原种、良种场,丰富物种遗传多样性是资源保护的基础.     

利用RAPD和AFLP标记初步构建太平洋牡蛎的遗传连锁图谱

利用RAPD和AFLP标记 ,以一回交家系 [(Miyagi×Hiroshima)×Miyagi]为作图群体 ,构建了太平洋牡蛎的遗传连锁图谱。用经过筛选的 3 3个RAPD引物和 1 1个AFLP引物组合 ,对父母本和 80个子代个体进行了遗传分析。共得到母本分离标记 1 93个 ,其中 1 44个符合1∶1孟德尔分离规律。父本分离标记 1 5 6个 ,其中 1 1 1个符合 1∶1孟德尔分离规律。雌性框架图包括 99个遗传标记 ,定位在 1 2个连锁群中 ,覆盖 985 2cM ,标记间平均间隔 1 1 3cM。另外有 3个三联体 ,7个连锁对 ,图谱共覆盖 1 1 6 5 7cM。雄性框架图包括 72个遗传标记 ,分布在 8个连锁群 ,覆盖 81 1cM ,标记间平均间隔 1 2 7cM。另外有 4个三联体 ,3个连锁对 ,图谱共覆盖93 1 8cM。     

高识别力的微卫星标记系统在鳜属(Siniperca)物种鉴定中的应用

根据自行分离的翘嘴鳜微卫星序列（GenBank登录号：DQ789247-DQ789306）设计并合成20对微卫星引物,对鳜属鱼类4个物种即翘嘴鳜、大眼鳜、斑鳜和暗鳜共80个个体进行了物种鉴定分析.结果表明,20对微卫星引物共检测到293个等位基因,大小在80-301bp之间.它们在4个物种中的多态性位点百分率分别为90%、75%、85%和85%,累积个体识别率和非父排除率均达到0.9999,属于高识别力的微卫星遗传标记系统,可以用来进行鳜属鱼类物种的鉴定分析.UPGMA聚类分析表明,翘嘴鳜与暗鳜之间亲缘关系最近,可归属于第Ⅰ类;大眼鳜为第Ⅱ类;斑鳜独自为第Ⅲ类.本研究可为鳜属鱼类的分类及进化关系、群体遗传结构分析等提供理论支持,为野生原种鳜类遗传多样性的监测和评估以及鳜类优良的种质资源得到合理保护和开发利用奠定基础.     

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po