Application of Microbial BOD Sensors in Marine Monitoring

A strain of yeast, which can endure high osmotic pressure, is employed for the sensitive material of the microbial BOD sensor. Two immobilization methods are used, I.e. Calcium alginate gel be ads and PV A gel beads. The results show that the PVA gel beads is better. The influences of osmosis and heavy metal ions on the yeast entrapped in the PVA gel beads are also studied in the experiment.     

口虾蛄(Oratosquilla oratoria)同工酶的组织特异性及生化遗传分析

采用聚丙烯酰胺凝胶垂直平板电泳方法，研究分析了口虾蛄(Oratosquilla oratoria)肌肉、眼球、腹鳃、心脏和肝胰腺5种器官或组织中LDH、ADH、MDH、SDH、MEP、POD、AMY、GDH、SCD、EST、GLDH和SOD共12种同工酶的表达情况。结果表明，口虾蛄同工酶的表达具有明显的组织特异性。对12种同工酶进行生化遗传分析，获得了基本酶谱。12种同工酶共记录出35个基因座位，其中11个基因座位Ldh-3、Adh-1、Sdh-2、Est-1、Est-2、Est-3、Mdh、Mep-7、Gldh-1、Gldh-2和Scd-1为多态，其多态座位比例为31.43％。     

Screening model for volatile pollutants in dual porosity soils

This paper develops mass fraction models for transport and fate of agricultural pollutants in structured two-region soils. Mass fraction index models, based on a semi-infinite domain solution, are derived that describe leaching at depth, vapor losses through soil surface, absorption, and degradation in the dynamic- and stagnant-water soil regions. The models predict that leaching is the result of the combined effect of the upward vapor-phase transport relative to downward advection, residence time relative to half-life, dispersion, and lateral diffusive mass transfer. Simulations show that leached fraction of volatile compounds does not always decrease monotonically with increased residence time relative to the pollutant half-life, as a result of complex interactions among the different physical and biochemical processes. The results show that leaching, volatilization, and degradation losses can be affected significantly by lateral diffusive mass transfer into immobile-water regions and advection relative to dispersion (i.e. Peclet number) in the mobile-water regions. It is shown that solute diffusion into the immobile phase and subsequent biochemical decay reduces leaching and vapor losses through soil surface. Potential use of the modified leaching index for the screening of selected pesticides is illustrated for different soil textures and infiltration rates. The analysis may be useful to the management of pesticides and the design of landfills.     

华南下二叠统层状硅岩的形成及意义

华南地区下二叠统的层状硅岩分布较稳定，厚度一般为几十米，含有数量不等的放射虫和海绵骨针等，并据此可分为放射虫硅岩、海绵骨针硅岩和贫化石硅岩。化学成分特点和硅氧同位素值，具有生物化学成因硅岩的特征，与火山成因和热水成因的硅岩也有一定联系。层状硅岩的形成明显受断裂控制，硅质生物、上升洋流和水平洋流则是硅质富集、保存和沉淀的主要因素，而海底火山和热水活动的参与也起到一定促进。层状硅岩中有机碳含量丰富，平均０．６９％，是潜在的有利烃源岩。     

凡纳滨对虾(Litopenaeus vannamei)继饥饿后恢复生长期间生化组成及能量收支的动态变化

采用氧弹式热量计和常规的动物生化成分分析方法,测定凡纳滨对虾在恢复生长过程中生化组成和能量分配模式的动态变化。结果表明,饥饿状态下,虾体干重、脂肪含量和能值下降,水分和灰分含量升高,但蛋白质含量没有明显改变。恢复投喂后,能量特定生长率显著提高,而虾体各生化组成在不同时间内均恢复到对照组水平。饥饿使对虾恢复生长过程特别是其前期的能量分配模式发生极显著变化。对虾的摄食能分配于生长的比例增大,而损失于代谢消耗的比例则相应减少;摄食能的增加和消化吸收率提高则仅发生在恢复生长的初期。可以认为,凡纳滨对虾继饥饿后的补偿生长效应主要出现在恢复生长的前期,并随时间延长逐步减弱,其生理机制最主要是得益于代谢耗能比例的减少。     

Effect of size, tissue parts and location on six biochemical markers in the green-lipped mussel, Perna viridis

The effect of size and tissue parts as well as site locations on a suite of six anti-oxidative biochemical markers, viz. the enzyme degrading neural transmitters acetylcholinesterase (AChE), the Phase II transforming enzyme glutathione-S-transferase (GST), the anti-oxidative enzymes superoxide dismutase (SOD) and catalase (CAT), the lipid metabolite malondialdehyde (MDA) and the ratio of CAT/MDA, in the bivalve mollusc, Perna viridis, was studied. Field mussels were collected from the piers of University of Science and Technology (UST), Pak Sha Wan or Hebe Haven (PS) and Sai Kung (SK) in Port Shelter, Hong Kong. Statistically, ANOVA showed that the activity of all six biochemical markers were generally tissue dependent, size independent and location dependent. Tukey multi comparison test indicated that the biochemical activities fell into two groups, with SK the highest activity group in most cases. The significance of these findings in relation to long-term coastal water biomonitoring is discussed.     

A review on reflective remote sensing and data assimilation techniques for enhanced agroecosystem modeling

During the last 50 years, the management of agroecosystems has been undergoing major changes to meet the growing demand for food, timber, fibre and fuel. As a result of this intensified use, the ecological status of many agroecosystems has been severely deteriorated. Modeling the behavior of agroecosystems is, therefore, of great help since it allows the definition of management strategies that maximize (crop) production while minimizing the environmental impacts. Remote sensing can support such modeling by offering information on the spatial and temporal variation of important canopy state variables which would be very difficult to obtain otherwise.In this paper, we present an overview of different methods that can be used to derive biophysical and biochemical canopy state variables from optical remote sensing data in the VNIR-SWIR regions. The overview is based on an extensive literature review where both statistical–empirical and physically based methods are discussed. Subsequently, the prevailing techniques of assimilating remote sensing data into agroecosystem models are outlined. The increasing complexity of data assimilation methods and of models describing agroecosystem functioning has significantly increased computational demands. For this reason, we include a short section on the potential of parallel processing to deal with the complex and computationally intensive algorithms described in the preceding sections.The studied literature reveals that many valuable techniques have been developed both for the retrieval of canopy state variables from reflective remote sensing data as for assimilating the retrieved variables in agroecosystem models. However, for agroecosystem modeling and remote sensing data assimilation to be commonly employed on a global operational basis, emphasis will have to be put on bridging the mismatch between data availability and accuracy on one hand, and model and user requirements on the other. This could be achieved by integrating imagery with different spatial, temporal, spectral, and angular resolutions, and the fusion of optical data with data of different origin, such as LIDAR and radar/microwave.     

Interannual variability in the biochemical composition of newly hatched larvae of the spider crab Maja brachydactyla (Decapoda,Majidae)

The spider crab Maja brachydactyla is an important commercial species in Europe and supports intensive fisheries in the NE Atlantic. A field survey was performed to assess long‐term and consecutive interannual (2005–2010) variation of the biochemical composition of newly hatched larvae of M. brachydactyla. Larval biochemical profiles differed significantly among years, with pronounced differences being recorded in 2010. Differences among batches of newly hatched larvae were mainly explained by the contribution of triacylglycerols and, to a lesser degree, by protein and lipid content. The use of different nutrition indices is discussed. The biochemical composition of newly hatched larvae from M. brachydactyla was highly variable, even though surveyed broodstock was obtained from the same local population and was always sampled during the same season. The unpredictable biochemical profiles of newly hatched larvae may condition their survival and recruitment. This largely overlooked aspect of larval variability should be considered in future fisheries management strategies and captive production of marine organisms that still rely on wild seeds.     

养殖海鲡肌肉的生化组成

对海鲡肌肉的营养成分含量及氨基酸、脂肪酸、无机质组成进行了研究，结果表明，海鲡的蛋白质和脂肪含量分别为21.5%和5.5%，必需氨基酸和鲜味氨基酸分别占氨基酸总量的40.9%和37.8%，不饱和脂肪酸占脂肪酸总量的65.2%，其中C205和C226的含量分别为4.5%和12.0%，3.4kg阶段的养殖海鲡肌肉的必需氨基酸和呈味氨基酸的比率最高。     

Effect of Polianthes tuberosa (Amaryllidaceae) on the Reproduction and Biochemical Parameters in the Ovotestis of Snail Lymnaea acuminata (Mollusca,Pulmonata)

The present study elucidates the effect of powder of Polianthes tuberosa bulb and its sapogenins tigogenin and hecogenin on the reproduction of the snail Lymnaea acuminata. It has been reported that the two sapogenins tigogenin and hecogenin caused a significant reduction in fecundity, hatchability, and survival of young snails. Exposure to sublethal concentration caused a small but significant reduction in protein, amino acids, nucleic acids, and phosphatase activities in gonadal tissue of treated snails. Withdrawal after 7 days indicates that the effect of powder of P. tuberosa bulb, tigogenin, and hecogenin on the reproduction and different biochemical parameters is reversible.