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1.
Bacterioplankton play critical roles in biogeochemical cycling. Although spatial and temporal variations in bacterioplankton community compositions (BCCs) within individual habitat have been reported, knowledge gaps remain for studies conducted within different habitats. In this work, we examined the seasonal and spatial variability of BCCs in Nanfei River and Lake Chaohu which had significant environmental heterogeneity using a high-throughput sequencing technique of 16S rRNA gene amplicons. The results showed that spatial variation has a more obvious impact on the BCCs than seasonal changes. The microbial diversity gradually decreased and BCCs changed obviously along water flow direction from Nanfei River to the western and estern parts of Lake Chaohu over all seasons. LEfSe analysis showed that Nanfei River had higer abundance of species belonging to the orders Rhodocyclales, Methylococcales, Campylobacterales and Flavobacteriales, samples from eastern part of Lake Chaohu were abundant in taxonomies including the order Rickettsiales, while the western part had high abundance of taxonomies belonging to the order Chroococcales. The redundancy analysis (RDA) indicated that BCCs in Nanfei River were associated with high nutrient (TP, PO4-P, TN, NH3-N, NO2-N and NO3-N) concentrations and electrical conductivity. Variance partitioning RDA analysis indicated that the combined effects of all variables may be most important affecting BCCs. This study may provide a framework for modeling the change in bacterioplankton communities through different habitats from a river to lake.  相似文献   
2.
We analysed total bacterial number and mean volume of cells at three sites in each of ten floodplain lakes in the Middle Basin of the Biebrza River, North-Eastern Poland to test bacterioplankton communities change according to the distance to the river. The composition of the bacterial communities was determined by fluorescent in situ hybridization method. Total number of bacteria in the lakes ranged from 4.0 to 7.48 cells × 106 mL−1 with dominance by Actinobacteria, the contribution of which was positively correlated with water level. Old river channels (side-arms) featured Alpha- and Gammaproteobacteria. The community of Betaproteobacteria was limited by concentration of dissolved organic carbon. Archaea, in spite of a minor role (<3.65% of DAPI-4′,6-diamidino-2-phenylindole) in the communities, showed a positive relation to floodplain lake isolation. Multivariate analysis demonstrated that bacterioplankton in riverine lakes was similar to that in rivers, while lakes with limited water exchange showed a similarity to fertile lakes. Water level and nutrients were among the factors determining bacterial community structure.  相似文献   
3.
Expression of intracellular ammonium assimilation enzymes were used to assess the response of nitrogen (N) metabolism in bacterioplankton to N-loading of sub-tropical coastal waters of Key West, Florida. Specific activities of glutamine synthetase (GS) and total glutamate dehydrogenase (GDHT) were measured on the bacterial size fraction (<0.8 μm) to assess N-deplete versus N-replete metabolic states, respectively. Enzyme results were compared to concentrations of dissolved organic matter and nutrients and to the biomass and production of phytoplankton and bacteria. Concentrations of dissolved inorganic N (DIN), dissolved organic N (DON), and dissolved organic carbon (DOC) positively correlated with specific activities of GDHT and negatively correlated with that of GS. Total dissolved N (TDN) concentration explained 81% of variance in bacterioplankton GDHT:GS activity ratio. The GDHT:GS ratio, TDN, DOC, and bacterial parameters decreased in magnitude along a tidally dynamic trophic gradient from north of Key West to south at the reef tract, which is consistent with the combined effects of localized coastal eutrophication and tidal exchange of seawater from the Southwest Florida Shelf and Florida Strait. The N-replete bacterioplankton north of Key West can regenerate ammonium which sustains primary production transported south to the reef. The range in GDHT:GS ratios was 5–30 times greater than that for commonly used indicators of planktonic eutrophication, which emphasizes the sensitivity of bacterioplankton N-metabolism to changes in N-bioavailability caused by nutrient pollution in sub-tropical coastal waters and utility of GDHT:GS ratio as an bioindicator of N-replete conditions.  相似文献   
4.
微生物群落是湖泊生态系统的重要组成部分,其结构和功能的稳定性对于生态系统正常执行功能和服务具有至关重要的作用。以营养富集和盐度增加为特征的全球变化深刻影响湖泊生态系统,揭示这些因素对微生物群落结构及其稳定性的影响机理具有重要意义。本研究以浮游细菌群落为研究对象,开展2个营养水平和3个盐度干扰强度水平(对照组、3‰和9‰)的室内模拟实验;基于同一时间点的实验组和对照组之间细胞密度的差异,计算反应力、抵抗力、恢复力和时间稳定性等多个稳定性指标,尝试揭示群落的稳定性机制;借助细菌16S rRNA基因高通量测序对群落结构进行分析,阐明不同营养水平下浮游细菌群落不同类群对盐度干扰的响应。研究发现,浮游细菌的细胞密度和物种丰富度在盐度干扰后降低,对盐度敏感类群相对丰度减少;盐度干扰后水体中溶解性有机碳增加,促进了适应干扰后环境的浮游细菌生长。更高的干扰强度显著降低了细胞密度,导致了更低的抵抗力;盐度干扰和营养水平在反应力、恢复力和时间稳定性方面存在显著交互作用,在9‰盐度干扰下,高营养水平群落表现出最低的稳定性。多数稳定性指标之间表现出了显著的正相关,可以协同指示浮游细菌群落的稳定性;抵抗力和恢复力之间并不显著相关,但是可以从不同角度反映群落稳定性。未来淡水生态系统的富营养和盐度增加将显著降低浮游细菌群落稳定性,多个稳定性指标的应用和高频次观测有助于了解浮游细菌群落对干扰的响应与稳定性机制。  相似文献   
5.
根据2008年夏季(7月)在大亚湾进行的现场综合调查资料,分析表层海水浮游细菌生物量的水平分布特征,探讨细菌生物量与环境变量的关系。大亚湾夏季浮游细菌生物量变化范围为14.00~118.80μg·L-1,海区平均值为55.23±29.43μg·L-1。大亚湾夏季细菌生物量水平分布大体上呈现南部高于北部的趋势,在北纬22°35′以北和以南的海区均各自表现为东南部高于西北部的趋势。水深、硝酸盐、铵盐、亚硝酸盐和总碱度等5个参数与浮游细菌生物量之间存在着中等水平的相关性(0.15相似文献   
6.
We studied the microbial food web in the upper 100 m of the water column in iron-limited sub-Antarctic HNLC waters south-east of New Zealand in the SAGE experiment in 2004, with focus on bacterioplankton. Samples were collected daily from inside and outside the iron enriched patch. Short term enrichment experiments were conducted on board in 4 L polycarbonate bottles with water outside the iron enriched patch to study single and combined effects of micronutrient additions on microbial food web. Low bacterial growth was recorded in the study area with community turnover times of 50 h or more during the study period. Measurements of bacterial standing stocks and production rates in the study show minor responses to the large scale iron enrichment, with increase in rates and stocks after the first enrichment and at the end of the study period after the third iron enrichment when solar radiation increased and wind mixing decreased. The average daily bacterial production rates were 31.5 and 33.7 mgCm−2 d−1 for the OUT and IN stations, respectively; thus overall there was not a significant difference between the control and the iron-enriched patch. In the bottle experiments bacterial thymidine incorporation showed responses to single iron and silicic acid enrichments and a major growth response to the combined iron and sucrose enrichments. Phytoplankton chlorophyll-a showed clear stimulation by single additions of iron and silicic acid and silicic acid enhanced the iron impact. Cobalt additions had no effect on bacteria growth and a negative effect on phytoplankton growth. Low bacterial in situ growth rates and the enrichment experiments suggest that bacteria are co-limited by iron and carbon, and that bacterial iron uptake is dependent on carbon supply by the food web. With the high iron quota (??mol Fe mol C−1) bacteria may scavenge considerable amounts of the excess iron, and thus influence the relative importance of the microbial food web as a carbon sink.  相似文献   
7.
应用16S rRNA基因末端限制性片段长度多态性技术和多元统计方法,探究了南京玄武湖浮游细菌群落结构的季节性变化,并找出了浮游细菌群落组成分布的主要影响因子.结果表明,玄武湖4个季节中夏季Invsimpson指数、Shannon-Wiener指数和Pielou指数最高,而春季最低.非度量多维尺度分析结果显示浮游细菌群落结构存在着明显的季节性变化.Mantel检验、典范对应分析、变量分割分析表明影响玄武湖细菌群落结构最显著的环境因子为温度,其次为pH.营养盐浓度对玄武湖细菌群落结构的影响不显著.总的来说,本研究将有助于更好地理解富营养化水体中的浮游细菌群落组成的季节变化规律.  相似文献   
8.
太湖北部湖区水体中浮游细菌的动态变化   总被引:2,自引:7,他引:2  
冯胜  高光  秦伯强  陈默 《湖泊科学》2006,18(6):636-642
2003年1月-12月对太湖北部湖区8个采样点进行了每月1次、为期一年的水体中浮游细菌数量的测定,并同步对溶解性有机碳(DOC),总氮,总磷,叶绿素a和温度进行了测定,结果表明:浮游细菌数量存在明显的季前变化和空间差异,夏秋季浮游细菌数量比冬春季高,最高值出现在夏季的7月份,平均值为7.43×106cell/ml,最低值出现在3月份,平均值为3.14×106cells/ml,最低值与最高值差异达73%;污染严重的河口区浮游细菌数量明显高于湖心区,最高值出现在河口的6#点,平均值为5.51×106cells/ml,比湖心区最低值8#点高83.2%,并呈现从河口、湾内至湖心随水体污染程度减轻而逐步递减的趋势;浮游细菌数量与温度和浮游植物量显著相关.而与水体中营养盐无关,预示着太湖水体中的营养盐已处于较高的水平,不再是浮游细菌生长的限制因素,而来源于浮游植物的有机碳可能是其生长的重要碳源.  相似文献   
9.
Bacterioplankton abundance (BA) and biomass (BB) from the eutrophic Pearl River Estuary (PRE) to the oligotrophic northern South China Sea (NSCS) were studied in the wet season. BA was significantly higher (p < 0.05) in PRE (12.51 ± 3.52 × 108 cells L−1), than in the continental shelf neritic province (CSNP, 4.95 ± 2.21 × 108 cells L−1) and in the deep oceanic province (OP, 3.16 ± 1.56 × 108 cells L−1). Nutrient-replete PRE waters (DIN > 100 μM and PO4 > 1 μM) resulted in high chl a and BB, whereas nutrient-depleted offshore waters (DIN <5 μM and PO4 < 0.5 μM) had low biomass. Temperature (>26 °C) was not the controlling factor of BA. BB was significantly correlated with chl a biomass both in PRE and NSCS. The bacteria to phytoplankton biomass (BB/PB) ratio increased clearly along the gradient from near-shore PRE (0.15) to offshore CSNP (0.93) and deep OP (2.75), indicating the important role of small cells in the open ocean compared to estuarine and coastal zones.  相似文献   
10.
The purpose of this study was to measure the phytoplankton production (PP) and bacterial production (BP) simultaneously in seven lakes in the middle Rio Doce basin (southeast Brazil) during the dry and the rainy seasons. Limnological monitoring was conducted from 1999 to 2001 as well as both PP (radioactive carbon fixation) and BP (3H-Leucine incorporation) in four specific depths (100%, 10%, 1% of incident radiation, and aphotic zone). Furthermore, trophic state of the lakes was analyzed, considering the index proposed by Salas and Martino (1991) and Carlson (1977). In general, some parameters increased during the rainy season such as pH, conductivity, chlorophyll and total nitrogen. In the period of 1999/2000, the rain caused PP reduction in the lakes, excepting Carioca and Águas Claras Lake, but in the years 2000/2001, the lakes did not show a similar pattern. In contrast, the rainy season of both years caused an expressive bacterioplankton production enhancing. Using Salas and Martino index, we observed that some lakes were affected by the seasonal runoff, therefore the typologie of these lakes changed from oligotrophic to mesotrophic conditions, otherwise considering the Carlson index all lakes were classified as eutrophic in both dry and rainy seasons. Indeed, Salas and Martino index demonstrated to be more appropriate to determine the trophic status of the lakes. Finally, our data indicate that until now, in spite of the lakes location, protected and unprotected area, the lakes still maintain their natural characteristics.  相似文献   
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