The stratiform Cu–Co ore mineralisation in the Katangan Copperbelt consists of dispersed sulphides and sulphides in nodules
and lenses, which are often pseudomorphs after evaporites. Two types of pseudomorphs can be distinguished in the nodules and
lenses. In type 1 examples, dolomite precipitated first and was subsequently replaced by Cu–Co sulphides and authigenic quartz,
whereas in type 2 examples, authigenic quartz and Cu–Co sulphides precipitated prior to dolomite and are coarse-grained. The
sulphur isotopic composition of the copper–cobalt sulphides in the type 1 pseudomorphs is between −10.3 and 3.1‰ relative
to the Vienna Canyon Diablo Troilite, indicating that the sulphide component was derived from bacterial sulphate reduction
(BSR). The generation of during this process caused the precipitation and replacement of anhydrite by dolomite. A second product of BSR is the generation
of H2S, resulting in the precipitation of Cu–Co sulphides from the mineralising fluids. Initial sulphide precipitation occurred
along the rim of the pseudomorphs and continued towards the core. Precipitation of authigenic quartz was most likely induced
by a pH decrease during sulphide precipitation. Fluid inclusion data from quartz indicate the presence of a high-salinity
(8–18 eq. wt.% NaCl) fluid, possibly derived from evaporated seawater which migrated through the deep subsurface. 87Sr/86Sr ratios of dolomite in type 1 nodules range between 0.71012 and 0.73576, significantly more radiogenic than the strontium
isotopic composition of Neoproterozoic marine carbonates (87Sr/86Sr = 0.7056–0.7087). This suggests intense interaction with siliciclastic sedimentary rocks and/or the granitic basement.
The low carbon isotopic composition of the dolomite in the pseudomorphs (−7.02 and −9.93‰ relative to the Vienna Pee Dee Belemnite,
V-PDB) compared to the host rock dolomite (−4.90 and +1.31‰ V-PDB) resulted from the oxidation of organic matter during BSR. 相似文献
We monitored near-surface atmospheric fallout (15-cm above ground) and soil solution (at 15, 35 and 55 cm below ground) derived nanoparticles over an 8-month period by collecting the particles directly onto TEM grids in anthropogenically-influenced (vineyard) and pristine (native forest) sites in France. Particle clusters trapped on the grid were selected randomly and individual particles were binned into eight different groups (euhedral clays, weathered clays, salts, oxi-hydroxides, bacteria, non-living organic matter, aggregates and undetermined). Bacteria represent 9–23% of the collected nanoparticle area (ave. 9.4% and 18% for two atmospheric collection sites and ave. 23% for soil infiltration samples). Bacteria were most often associated with non-living organic matter and comprised a variety of morpho-types. Interestingly, 45% of all the bacteria analyzed by transmission electron microscopy and electron dispersive spectroscopy (TEM-EDX) showed the presence of intracellular grains significantly enriched in lead and phosphorus. Intracellular sequestration of Pb into polyphosphate bodies has been observed in the laboratory, but this is the first observation of this phenomenon in a natural environment. Furthermore, this suggests that microbial-bound Pb may be an important transport mechanism in subsurface environments. 相似文献
The objective of this study was to examine the redox reactions and other transformations of mercury (Hg) species in surface waters, and the factors determining the rates of these reactions. For the redox studies completed at the Chesapeake Biological Laboratory (CBL), two isotopes (199HgII and 202Hg0) were added into different types of filtered water (fresh to seawater) to examine the oxidation and reduction reactions. Further studies of both the redox reactions and methylation/demethylation reactions of Hg were conducted with unfiltered water on board research vessels during cruises in May and July 2005 on the Chesapeake Bay and shelf. While CH3199HgII was added to allow the examination of demethylation, 201HgII was used to examine both reduction and methylation, and 202Hg0 was used to examine oxidation. Overall, the results showed that both Hg oxidation and reduction were simultaneously occurring and were photochemically mediated in the waters investigated. In contrast to the previously assumed “unreactive” nature of Hg0, the studies found that the magnitude of the rate constant for Hg0 oxidation was greater than that for reduction, indicating its importance in estuarine and coastal waters. In addition, both experiments at CBL and on board ship showed that HgII reduction was similar in magnitude, suggesting that biotic processes were relatively unimportant. While no measurable methylation occurred during the incubation period during the on board studies, concentration of CH3199HgII decreased over the time during the experiments. It appeared that the demethylation processes were not dominantly photochemically driven, but could be microbially mediated. Further studies are needed in order to help better understand Hg redox and transformations in natural water systems. 相似文献
The Atacama trench, the deepest ecosystem of the southern Pacific Ocean (ca. 8000 m depth) was investigated during the Atacama Trench International Expedition. Sediments, collected at three bathyal stations (1040–1355 m depth) and at a hadal site (7800 m) were analyzed for organic matter quantity and biochemical composition (in terms of phytopigments, proteins, carbohydrates and lipids), bacterial abundance, biomass and carbon production and extracellular enzymatic activities. Functional chlorophyll-a (18.0±0.10 mg m−2), phytodetritus (322.2 mg m−2) and labile organic carbon (16.9±4.3 g C m−2) deposited on surface sediments at hadal depth (7800 m) reached concentrations similar to those encountered in highly productive shallow coastal areas. High values of bacterial C production and aminopeptidase activity were also measured (at in situ temperature and 1 atm). The chemical analyses of the Atacama hadal sediments indicate that this trench behaves as a deep oceanic trap for organic material. We hypothesize that, despite the extreme physical conditions, benthic microbial processes might be accelerated as a result of the organic enrichment. 相似文献
In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance. 相似文献
Recently, bromodeoxyuridine (BrdU) has been successfully applied to the measurement of bacterial productivity as an alternative
to tritiated thymidine (3H-TdR), which is widely used but often restricted by regulations, particularly in field settings. Here, I report improvements
to existing BrdU methods to simplify procedures and increase sensitivity. The feasibility of the method was tested measuring
bacterial production in low-productive waters. The method provided radioisotope-free measurements of bacterial production
rates at shorter (∼1 h) on-board processing time of samples than previously reported procedures. It was applicable to the
detection of rates ranging from 0.021 to 2.7 pmol BrdU l−1h−1. BrdU incorporation rates measured by immunoassay showed a statistically significant correlation with 3H-TdR incorporation rates measured by radioassay (r = 0.74, n = 24, p < 0.001). The linear regression obtained (BrdU = 0.80[3H-TdR] − 0.016) showed a similar relationship to previously reported regressions (BrdU = 0.65[3H-TdR] + 0.12, [3H-BrdU] = 0.69[3H-TdR] − 0.81). There were no statistically significant differences among these regression lines. These results suggest that
the method described here provides a non-radioisotopic productivity measurement of bacteria in oceanic epipelagic waters,
while retaining continuity of the data with other existing 3H-TdR and BrdU methods. 相似文献
As a result of the collapse of the Aznalcollar mine tailings dam, a large extension of the Guadiamar valley in SW Spain was covered with a layer of pyrite sludge. Although most of the sludge was removed, a small amount remains in the soil, constituting a potential source of water pollution. A column experiment was carried out in order to determine the rate of sludge oxidation in the soil, and the existence of metal retention processes. The column was filled with a mixture of sludge and a sandy soil common in the region. At different time intervals, the column was leached with water and the resulting solution analysed.
The pH of the water dropped to values around 2 after 260 days and then remained constant due to the buffering role of silicate dissolution. The concentration of Zn, Cd and Co in the leachates matched the expected values from flow-through experiments at atmospheric oxygen pressure. This indicates that oxygen diffusion in the pores was complete. Moreover, no efficient processes existed for retaining Zn, Cd and Co, which formed soluble salts and were entirely incorporated into the infiltrating water. During the first 2 months of the experiment, when pH was higher than 4.5, the concentrations of Fe and Al were very low. Saturation indices showed that the solution was in equilibrium with amorphous Fe(OH)3 and Al(OH)3. Subsequently, at lower pH values, jarosite [(Na,K)Fe3(SO4)2(OH)6] formed. This solid phase was identified by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Jarosite was also responsible for the depletion of As, Pb, Sb and probably Tl in the water resulting from infiltration. 相似文献