4种鲷科鱼种内细胞色素b基因序列的保守性

对真鲷 (Pagrosomusmajor)、黄鳍鲷 (Sparuslatus)、黑鲷 (S .macrocephalus)和平鲷 (Rhabdosar gussarba )的线粒体DNA细胞色素b 4 0 5bp序列进行测定。结果发现 ,4种鲷科鱼种内碱基的变异较低 ,真鲷为 0 .2 5% ,黄鳍鲷为 0 .74 % ,黑鲷和平鲷均为 0 ;真鲷有 4种单倍型 ,黄鳍鲷有 2种单倍型 ,黑鲷和平鲷分别为 1种单倍型 ,且单倍型间变异位点很少 ,真鲷有 3个变异位点 ,黄鳍鲷仅有 1个变异位点 ,而黑鲷和平鲷无变异位点。结果表明 ,细胞色素b基因在这 4种鲷科鱼种内是相当保守的。     

蜕壳素对凡纳滨对虾生长及表观消化率的影响

研究了外源性蜕皮激素对凡纳滨对虾生长和表观消化率的影响 ,实验时在基础饲料中分别添加 0 .1%、0 .2 %、0 .4 %的两种市售蜕壳素产品 ,制成 6种外源性脱壳素配合饲料 ,不添加的基础饲料作为对照组 ,试验在 2 6 0L玻璃钢循环水养殖系统中 ,每个剂量组设 3个重复 ,8周生长试验结果表明 :市售蜕壳素产品能有效地促进对虾生长 ,试验结束时 ,试验组虾体增重分别较对照组增加 16 .8%、7.5 2 %、5 .9%、11.4 %、2 9.0 7%、16 .5 3% ,但是凡纳滨对虾对饲料粗蛋白的表观消化率没有明显地提高 ,凡纳滨对虾对脂肪的表观消化率较低。推算凡纳滨对虾饲料中最佳蜕皮酮添加量为 6 0mg/kg。     

STUDY ON THE INTERACTION BETWEEN NDVI PROFILE AND THE GROWING STATUS OF CROPS

Daily and ten-day Normalized Difference Vegetation Index(NDVI) of crops were retrieved from meteorological statellite NOAA AVHRR images ,The temporal variations of the NDVI were analyzed during the whole growing season,and thus the principle of the interaction between NDIV profile and the growing status of crops was discussed,As a case in point,the relationship between integral NDVI and winter wheat yield of Henan Province in 1999 had been analyzed.By putting integral NDVI values of 60 sample counties into the winter wheat yield-integral NDVI coordination,scattering map was plotted. It demonstrated that integral NDVI had a close relation with winter wheat yield.These relation could be described with linear,cubic polynomial ,and exponential regression,and the cubic polynomial regression was the best way,In general ,NDVI reflects growing status of green vegetation ,so crop monitoring and crop yield estimation could be realized by using remote sensing technique on the basis of time serial NDVI data together with agriculture calendars.     

环境因子对L型、S型褶皱臂尾轮虫生长的影响

在实验室条件下，通过种群累积培养的方法，研究了不同盐度和小球藻(Chorella vulgaris)、亚心形扁藻(Platymonas subcordiformis)、微绿球藻(Nannochloris oculata)、波吉卵囊藻(Oocystis borgei)等不同饵料微藻对L型褶皱臂尾轮虫(Brachionus Plicatilis tipicus)和S型褶皱臂尾轮虫(Brachionus Plicatilis rotundiformis)生长的影响，探讨这两种轮虫在生态条件上的差异。结果表明：L型和S型褶皱臂尾轮虫对盐度的适应有显著的差异，生长的最适盐度分别为15和20，大水体培养时，其适宜生长的盐度范围分别是15～20和10～30。饵料微藻对L型和S型褶皱臂尾轮虫生长的影响一致。小球藻培养效果在4种饵料微藻中最好，其生长率明显高于微绿球藻和亚心形扁藻，波吉卵囊藻培养效果较差。     

A New Combination and Two Varieties of Polystigmate Gomphonema （Gomphonemaceae Bacillariophyta） from Heilongjiang Province, China

The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema tuttis Ehr. vat. latum Fan et Wang. var.nov. and Gomphonema acum/natum var. obtusum Fan et Bao, var. nov., that were distinguished by frustule morphology under LM. The samples were collected from freshwater of Heilongjiang Province, China.     

企鹅珍珠贝人工苗生长的初步观察

为了解企鹅珍珠贝生长规律，观察了壳高2.5～5.0mm的出池幼苗，按月测量其生长参数和成活率，以及环境因子。结果表明，企鹅珍珠贝生长最快的月份为7～11月和次年4～6月，壳长，壳高和壳宽月均增加量分别为3.8～13.0mm、4.7～11.2mm、2.3～3.8mm，月成活率97.8%～98.6%。企鹅珍珠贝生长最慢月份是11月至次年3月。     

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

厦门湾沉积物中有害有毒甲藻休眠孢囊及塔玛亚历山大藻的潜在危害

本工作通过分析厦门湾表层沉积物中的甲藻休眠孢囊，发现了1种有毒塔玛亚历山大藻(Alexandrium tamarense)休眠孢囊，及两种有害甲藻休眠孢囊：锥状斯氏藻(Scrippsiella trochoidea)、具刺膝沟藻(Gonyaulax spinifera)．对塔玛亚历山大藻进行了培养。并初步研究了该藻的毒性及贝类对其毒素的积累．研究结果表明，厦门海域沉积物中的塔玛亚历山犬藻是有毒的．它的较适宜生长环境条件是：水温20～25℃．盐度为25～30，光照度为3000～50001x．塔玛亚历山大藻在每年的4月26日至6月10日、10月11日至11月20日可能会形成有害藻华，危害厦门海域的海洋生物并可能威胁到人类的健康和生命安全．