Genetic Relationships Among One Non-Endemic and Two Endemic Mediterranean Triplefin Blennies (Pisces, Blennioidei)

Abstract. Three triplefin blennies occur sympatrically in the Mediterranean Sea; Tripterygion tripteronotus and T. melanurus are endemic, whereas T. delaisi is also found in the Eastern Atlantic. Although very similar in morphology, ecology and behaviour, some striking differences exist among reproductive strategies. Several authors proposed hypotheses on the evolution of these species. In order to enhance insight into the genetic structure of this genus, an enzyme electrophoretic survey was performed. Samples of 40 to 45 specimens of each species were collected near Calvi (Corsica, France). Twenty-four enzyme loci were analysed, of which 17 appeared polymorphic. T. delaisi showed a high level of average observed heterozygosity (H_o) of 0.140, as opposed to very low levels of 0.009 and 0.021 in T. tripteronotus and T. melanurus , respectively. Nei's genetic distance was 0.21 between T. delaisi and T. tripteronotus , 0.35 between T. delaisi and T. melanurus and 0.45 between T. tripteronotus and T. melanurus . We hypothesise that divergence of these species started before the Pleistocene and that the endemic species survived the Pleistocene glaciations in refugia within the Mediterranean. The possibility that T. tripteronotus and T. delaisi diverged through sympatric speciation is discussed.     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

软土海岸地区测量控制点标志的设立

分析了软土、湿地、滩涂地区特殊地理环境对测量控制点的影响;主要探讨了此类地区控制点标志的设计、施工。借助于建筑桩基技术和道桥施工方法,首次成功实现了某三角洲湿地滩涂地区统一布网。几年来,结合滩涂湿地测量、海岸防护和滩涂开发工程对部分控制点进行了初步检测,给出了部分检测数据。应用和检测均表明:桩基标石可较好地解决软土、湿地、滩涂地区控制点布设难题,显著提高软土海岸地区测量控制点标志的稳固性,从而延长控制网的可使用期。     

HLAFS和T63数值预报产品在暴雨分析中的应用

首次使用ＨＬＡＦＳ数值预报产品对黑龙江省暴雨进行分析，结合Ｔ６３数值预报产品，利用两者提供的物理一０８时和２０时的实况场和预报场格点资料，对黑龙江省一次暴雨过程进行分析，找出了暴雨发生的物理机制，指出深厚的水汽条件以及水汽的辐合，强烈的上升运动和不稳定能量的存贮和释放是产生暴雨的关键因素。     

Mesoscale Diagnosis of a Torrential Rainfall Caused by a Tropical Depression

On August 5, 2001, Shanghai was struck by a torrential rainfall due to the passage of a tropical depression (TD). The rainfall intensity has been the strongest in recent 50 years. In this paper, a set of mesoscale re-analyses data and the planetary boundary layer observation from a wind profiler are used to understand the possible mechanism of such a heavy rain. Results show that the outburst of a southerly jet in the lower atmosphere triggered the explosive development of cyclonically vertical vorticity in the region with steep potential temperature surfaces in front of the TD; while the cyclonic vorticity increased notably at higher levels due to the small atmospheric vertical stability of westerly currents in the vicinity of Shanghai. The simultaneous sharp development of cyclonic vorticity at different levels should be the main cause for the torrential rainfall.     

青藏高原腹地植物碳同位素组成对环境条件的响应

现代植物碳同位素组成是特定环境影响的结果,通过对植物碳同位素组成的研究可以揭示植物生长期环境信息。针对青藏高原腹地高寒草甸~高寒草原过渡区植被碳同位素组成进行研究;该区高山嵩草样δ13C值在-25.63‰~-27.95‰间,平均值-26.63‰;高寒草原区混合样δ13C值于-26.29‰~-27.73‰间,平均值-27.04‰。高山嵩草样δ13C值总体呈现由南东往北西方向正偏趋势,研究区北部高寒草原区混合植物样也呈现出由南向北富重碳同位素趋势。这些变化规律被认为是主要受降水环境影响的结果,而区域内降水条件的展布规律则是受高原夏季风运移方式的控制。对植物δ13C值与地理位置的回归分析表明,该区植被碳同位素组成与地理位置相关,高山嵩草样(r=0.44603,n=29,p<0.05)和混合样(r=0.8112,n=5,p<0.1)均表现出对区域降水环境条件的良好响应。据此,以该区植物δ13C值为背景,进行合理推算,拟定了研究区内干旱区和湿润区界限的位置。     

Divergence and flow direction as indicators of subsurface heterogeneity and stage‐dependent storage in alluvial floodplains

Assuming homogeneity in alluvial aquifers is convenient, but limits our ability to accurately predict stream‐aquifer interactions. Research is needed on (i) identifying the presence of focused, as opposed to diffuse, groundwater discharge/recharge to streams and (ii) the magnitude and role of large‐scale bank and transient storage in alluvial floodplains relative to changes in stream stage. The objective of this research was to document and quantify the effect of stage‐dependent aquifer heterogeneity and bank storage relative to changes in stream stage using groundwater flow divergence and direction. Monitoring was performed in alluvial floodplains adjacent to the Barren Fork Creek and Honey Creek in northeastern Oklahoma. Based on results from subsurface electrical resistivity mapping, observation wells were installed in high and low electrical resistivity subsoils. Water levels in the wells were recorded real time using pressure transducers (August to October 2009). Divergence was used to quantify heterogeneity (i.e. variation in hydraulic conductivity, porosity, and/or aquifer thickness), and flow direction was used to assess the potential for large‐scale (100 m) bank or transient storage. Areas of localized heterogeneity appeared to act as divergence zones allowing stream water to quickly enter the groundwater system, or as flow convergence zones draining a large groundwater area. Maximum divergence or convergence occurred with maximum rates of change in flow rates or stream stage. Flow directions in the groundwater changed considerably between base and high flows, suggesting that the floodplains acted as large‐scale bank storage zones, rapidly storing and releasing water during passage of a storm hydrograph. During storm events at both sites, the average groundwater direction changed by at least 90° from the average groundwater direction during baseflow. Aquifer heterogeneity in floodplains yields hyporheic flows that are more responsive and spatially and temporally complex than would be expected compared to more common assumptions of homogeneity. Copyright © 2012 John Wiley & Sons, Ltd.     

Phylogeography of the pelagic fish Seriola lalandi at different scales: confirmation of inter-ocean population structure and evaluation of southern African genetic diversity

The study investigated the global and regional phylogeography of the yellowtail kingfish Seriola lalandi by examining genetic diversity and population genetic structure of this species at inter-and intra-ocean level and on a regional scale. DNA fragments of two mitochondrial genes, cytochrome b (Cytb) and cytochrome c oxidase subunit I (COI) and one nuclear gene, recombination activating gene 1 (RAG1), were sequenced to investigate the global-scale phylogeography of this species. The population genetic structure within the South Pacific, as well as along the South African coastline, was examined further using six microsatellite markers. Three distinct clades were identified for S. lalandi, which correspond with previously described subspecies of the North-East Pacific, North-West Pacific and the Southern Hemisphere. Within the latter, additional divergence was observed between the South Pacific and the South-East Atlantic regions. Divergence estimates were indicative of a Pacific origin for S. lalandi populations, because of Pleistocene vicariant events. Microsatellite analyses revealed overall significant genetic differentiation between South African and South Pacific samples. This corroborates recent findings on the global phylogeography of the species. No population differentiation was observed within South Africa, indicating high levels of gene flow.     

不同定义的湿位涡分析及在台风中的诊断

本文对不同定义的湿位涡做了理论分析,并利用1522号台风“彩虹”的数值模拟结果对各种湿位涡进行了诊断。主要结论有:经典湿位涡、广义湿位涡和改进湿位涡的差异主要是由不同定义的位温造成的,相当位温、广义位温和修改位温的构成均是在位温基础上添加一显含水汽的附加量;经典湿位涡、广义湿位涡和改进湿位涡的构成均能分为干、湿分量两部分,其干分量表达式相同,都与Ertel干位涡的定义一样,水物质相变潜热的影响隐含在位温中;不同定义湿位涡的本质差异表现在不同的湿分量上,湿分量的表达式中显含了水物质的作用。对台风的诊断分析发现,改进湿位涡分布与Ertel干位涡非常相似,呈现中空分布的位涡塔结构,大值区对应眼墙内侧,改进湿位涡湿分量与经典湿位涡的湿分量分布相似,只是湿分量的绝对值更小,这反映了改进湿位涡既能保持干位涡的分布特征,其分布和演变可反映台风的结构和演变,又能合理地体现水汽分布的影响,所以在台风诊断中有更广泛的应用前景。经典湿位涡在低层表现为负值,这与水汽梯度的分布关系很大,但与垂直速度、潜热加热大值区等都没有很好的匹配关系,用其分析台风结构和演变具有一定局限性;广义湿位涡其形式较复杂,仅在近饱和区域才能发挥其诊断优势。