Arsenic Speciation in Lichens and in Coarse and Fine Airborne Particulate Matter by HPLC–UV–HG–AFS

A three-step sequential extraction procedure with Milli-Q, CaCl₂ and H₃PO₄ was applied for extraction of arsenic species in lichen transplants and airborne particulate matter (fine and coarse fractions). The samples used in this work were collected in 1994–1995 near coal-fired power plants. Both transplant lichens and airborne particulate matter were submitted to the same environment simultaneously. Arsenic species identification and quantification was performed by HPLC–UV–HG–AFS. Inorganic forms of arsenic (arsenite and arsenate) were present in significant amounts in most of the samples. Only in lichens also organic forms of arsenic (monomethyl arsonic acid and dimethyl arsinic acid) were identified which may indicate biotransformation of inorganic arsenic.     

Wind as a cooling agent: substrate temperatures are responsible for variable lithobiont‐induced weathering patterns on west‐ and east‐facing limestone bedrock of the Negev

Spatial variability in lithobiont‐induced weathering patterns on desert rocks is aspect‐dependent. While differences between the northern and southern aspects have been extensively studied, little is known concerning the differences between east‐facing (EF) and west‐facing (WF) aspects in deserts, including the Negev Desert. Whereas cobbles on both slopes are inhabited by endolithic lichens, epilithic lichens, which render the bedrock a smooth appearance, and free‐living cyanobacteria, which give the bedrock a rugged microrelief, predominate on WF and EF bedrock, respectively. Following previous research that regarded dew as the principal factor that determines lithobiont distribution, measurements of radiation, temperature, wind and dew were carried out during 2008–2009 in the Negev Desert. The data indicated that albeit slightly higher midday surface temperatures that characterize WF surfaces (cobbles and bedrock), nocturnal temperatures on these surfaces were significantly lower, therefore facilitating higher dew condensation. High amounts of dew result from the relatively rapid drop in temperatures (14:00–20:00) due to the afternoon northwesterly sea‐breeze wind (with a cooling rate of the WF bedrock being 52.9% higher than on EF bedrock, 2.6 °C h^?1 in comparison to only 1.7 °C h^?1), and facilitate the growth of high‐chlorophyll dew‐fed (and rain‐fed) epilithic lichens, which may act as bio‐protectors on WF bedrock. Lack of condensation on EF bedrock results in turn in the growth of rain‐fed free‐living cyanobacteria, responsible for high rock dissolution and subsequently for a rugged microrelief. By affecting the nocturnal bedrock temperatures, wind acts as a cooling agent, impacting in turn the amount of dew, and subsequently lithobiont composition and weathering patterns in the Negev Desert. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.     

Development of new spectral reflectance indices for the detection of lichens and mosses moisture content in the Hudson Bay Lowlands,Canada

Assessing moisture contents of lichens and mosses using ground‐based high‐spectral resolution spectrometers offers immense opportunities for a comprehensive monitoring of peatland moisture status by satellite/airborne imagery. This study investigates the impact of various moisture conditions of the lichens Cladina stellaris and Cladina rangiferina, and the mosses Dicranum elongatum and Tomenthypnum nitens on the spectral signatures obtained. Reflectance and moisture content measurements of these species were made in a laboratory setting, while maintaining the natural moisture conditions of the samples; once the moisture and spectral measurements were complete, the samples were returned to the field and placed in their natural setting, continuously receiving moisture from precipitation and groundwater and losing water through evaporation and drainage. Previously, we correlated the present spectral indices with the moisture contents of the above species, whereas the current study developed new species‐specific indices to improve the detection of the plants' moisture contents. The relationship between the plants' moisture content and the water table position was examined as well. It was found that the lichens are not responsive to variations in the water table position, whereas the mosses, specifically D. elongatum, are quite sensitive to changes in the water table position. Thus, the use of the mosses spectral indices may contribute to an indirect evaluation of the water table position. Overall, the results suggest that the unique spectral signatures of the above species can be detected by satellite and airborne imagery, whereas the mosses, can be used as indicators of peatlands moisture status. Copyright © 2010 John Wiley & Sons, Ltd.     

Element-Enrichment Factors in Lichens from Terceira, Santa Maria and Madeira Islands (Azores and Madeira Archipelagoes)

In this study, more than 20 minor and trace elements have been determined by INAA (instrumental neutron activation analysis), in different species of lichens in three Portuguese islands of the Central North Atlantic (Santa Maria, Terceira, and Madeira Islands). The foliose species Parmotrema bangii, Parmotrema robustum, Parmotrema crinitum, Flavoparmelia caperata, Platismatia glauca, and the fruiticose species Ramalina canariensis, Ramalina farinacea, Ramalina implectens, Ramalina pusilla, Cladonia furcata, Cladonia coniocraea, Usnea dasaea, Usnea esperantiana, were the collected species. Samples of superficial soils were also collected and the fraction below 64 m was analysed. The enrichment factors relative to this fraction using Al as the normalising element were calculated. Fruiticose and foliose lichens displayed different EF values concerning bromine and potassium. The first was more enriched in the foliose species while the latter was more enriched in the fruiticose species and was assigned to the physiological response. Enrichment was found for Mg, Cs, As, Hg, Na, Zn, and Cl, in all the species. Lichen-based concentration patterns were shown for Na, Al, Cl, Br, Sb, Ce, and Hg and compared to similar patterns in Portugal mainland. In general, the patterns compared well except for Cl, Na, and Br. Background levels of the epiphytic lichens collected in the islands were obtained.     

Lichens as Biomonitors of Depleted Uranium in Kosovo

This paper reports the results of a study using lichens as biomonitors to investigate the environmental distribution of depleted uranium (DU) at selected Kosovo sites as a consequence of the use of DU ordnance during the conflict of 1999. The results suggested that the use of DU ammunitions did not cause a diffuse environmental contamination in such a way to have caused a detectable U enrichment in lichens. Also isotopic ²³⁵U/²³⁸U measurements did not indicate the presence of DU particles in lichens, except for some samples at a heavily shelled site, which resulted contaminated by DU.     

Weakening of gneiss surfaces colonized by endolithic lichens in the temperate climate area of northwest Italy

A role of lithobionts in geomorphological processes is increasingly argued, but the spatio‐temporal scale of their impact is largely unexplored in many ecosystems. This study first characterizes in the temperate zone (northwest Italy) the relationships between lithobiontic communities including endolithic lichens and the hardness of their siliceous rock substrate (Villarfocchiardo Gneiss). The communities are characterized, on humid and xeric quarry surfaces exposed for decades and natural outcrops exposed for centuries, in terms of lichen and microbial constituents, using a combined morphological and molecular approach, and with regard to their development on and within the gneiss. A lichen species belonging to Acarosporaceae (Polysporina‐Sarcogyne‐Acarospora group, needing taxonomic revision) chasmoendolithically colonizes both the humid and xeric quarry surfaces, on which epilithic cyanobacterial biofilms and epilithic pioneer lichens respectively occur. Light and electron microscopic observations show the development of the endolithic thalli within rock microcracks and the hyphal penetration along crystal boundaries down to depths of 1 to 3 mm, more pronounced within the humid surfaces. Such colonization patterns are likely related to biogeophysical deterioration, while no chemical alteration characterizes minerals contacted by the endolithic lichen. By contrast, on natural outcrops, where the endolithic colonization is negligible, a reddish rind below epilithic lichens indicates chemical weathering processes. Schmidt Hammer measurements highlight that the endolithic lichens deeply affect the hardness of the gneiss (down to ?60% with respect to fresh controls and surfaces only colonized by cyanobacteria), exerting a significantly higher weakening effect with respect to the associated epilithic lithobionts. The phenomenon is more remarkable on humid than on xeric quarry surfaces and natural outcrops, where epilithic lichens are likely involved in long‐term hardening processes supporting surface stabilization. Endolithic lichens are thus active biogeomorphological agents at the upper millimetric layer of siliceous rocks in temperate areas, exerting their weakening action during the early decade‐scaled stages of surface exposure. Copyright © 2015 John Wiley & Sons, Ltd.     

Monitoring of Short-Term Heavy Metal Deposition by Accumulation in Epiphytic Lichens (Hypogymnia Physodes (L.) Nyl.)

Many lichens are very sensitive to air pollution due to their symbiotic nature. However, they are generally less sensitive to toxic effects of trace elements; therefore they can be used as accumulator organisms for estimating concentrations of these elements in the environment. Heavy metal accumulation in lichens is a commonly used bioindication method for assessing heavy metal ambient levels. An active biomonitoring method was used for the determination of short-term accumulation of zinc, lead, arsenic and cadmium by epiphytic lichens transplanted at different localities in Slovenia polluted by heavy metals. The atomic absorption spectrophotometry method was used for the determination of heavy metal content in lichen thalli. The content of heavy metals in lichens collected in the background area with clean air (Rogla, Pohorje Mountains) was used as the reference value. Lichens were transplanted from Rogla using the branch transplantation technique for a period of 6 months in the surroundings of Slovenian thermal power plants (Velenje, otanj, Zavodnje, Veliki Vrh, Vnajnarje, and Dobovec) and close to the lead and zinc ore smelter at erjav. The monthly accumulation of heavy metals was comparable within years at selected locations. Heavy metal pollution was the highest at erjav despite of remediation of lead and zinc ore smelter. The monthly accumulation of all four heavy metals was statistically significantly higher in lichens exposed at erjav than at other localities.     

Metabolised Tritium and Radiocarbon in Lichens and Their Use as Biomonitors

Lichens are now well known for their potential as bio-indicators of environmental pollution, but less is known about their suitability as quantitative biomonitors for atmospheric emissions of tritium (mainly as tritiated water, HTO) and radiocarbon (as ¹⁴CO₂) from nuclear facilities, although both radionuclides could result in non-trivial individual or collective radiation doses due to their high environmental mobility and their long half-lives. ³H and ¹⁴C are fixed in lichens mainly by the photosynthesis of the algal partner and then stored in the organic molecules of both alga and fungus. They have the advantage of allowing the monitoring of atmospheric water vapour without interference of soil water or soil organic substances as long as soil-inhabiting species are avoided. Lichens were collected in the surroundings of (military and civil) nuclear facilities, in areas away from any direct source of contamination and some were transplanted from a contaminated area to a non-contaminated one. The influence of the nuclear facilities can be clearly traced, sometimes in a spectacular way and the first results of analyses after transplants give a base for estimating the effective half-life of ³H in lichens.     

Sorption Characteristics of Inorganic, Methyl and Elemental Mercury on Lichens and Mosses: Implication in Biogeochemical Cycling of Mercury

The sorption studies of Hg²⁺, CH₃Hg⁺ and elemental mercury (Hg⁰) were carried out on lichen (Parmelia sulcata) and moss (Funaria hygrometrica) samples under laboratory conditions. Desorption studies with HCl indicate that inorganic mercury (Hg⁺²) and methyl mercury could be completely desorbed with 1 M HCl and 0.5 M HCl, respectively. Samples loaded with elemental mercury, however, needed 4–5 M HCl concentration for complete desorption of the adsorbed elemental mercury. When similar desorption studies were carried out with field samples collected around a thermometer factory with elevated levels of mercury (8 mg/kg), it was found that only about 10–15% of total mercury was desorbed with 1M HCl, while 4–5 M acid was required for complete desorption. We have tried to correlate this information to understand the transformations of mercury species that may occur either in the atmosphere or on the biomonitors. The results indicated that the elemental mercury, the principal form of mercury contamination around the thermometer factory, is converted into a strongly held form by some chemical binding agents on the surface of lichen/moss, or elemental mercury could diffuse into the cells of the lichen/moss, which then needs the stronger acid to release it. Sorption capacity studies suggest that the lichens and mosses can also be used as sorbent material for the decontamination of inorganic and methyl mercury from aqueous solutions.     

Lichens and mosses moisture content assessment through high‐spectral resolution remote sensing technology: a case study of the Hudson Bay Lowlands,Canada

Assessing moisture contents of lichens and mosses using ground‐based high‐spectral resolution spectrometers offers immense opportunities for a comprehensive monitoring of peatland moisture status by satellite/airborne imagery. This study investigates the impact of various moisture conditions of common subarctic lichen and moss species upon the spectral signatures obtained. The lichens are Cladina stellaris and Cladina rangiferina, and the mosses are Dicranum elongatum and Tomenthypnum nitens. Reflectance and moisture content measurements of these species were made in a laboratory setting, while maintaining the natural moisture conditions of the samples; once the moisture and spectral measurements were complete, the samples were returned to the field and placed in their natural setting, continuously receiving moisture from precipitation and groundwater and losing water through evaporation and drainage. Changes in reflectance of the visible to shortwave infrared (SWIR) range (400–2500 nm) at various moisture contents were examined, as well as the potential of current spectral reflectance indices to evaluate the plants' moisture contents was examined. Results indicate that the SWIR region is useful in identifying variations in plants moisture conditions, while the unique spectral signatures of the lichens and mosses in the visible and near‐infrared range suggest that these species may be detected by satellite and airborne imagery. Of current spectral indices, the normalized difference infrared index (NDII) was most successful in identifying the above plants' moisture content (details are discussed in the paper). Future study should focus on the development of improved moisture content spectral indices, as well as upscaling reflectance data and spectral indices. Copyright © 2010 John Wiley & Sons, Ltd.