海洋木栖真菌抗菌活性的初步研究

从福建厦门海沧、集美及漳州浮宫等地采集到的红树林等海洋潮间带的各种腐木样品中分离得到176株海洋木栖真菌,对其进行抗菌活性检测.其结果表明共有96株海洋木栖真菌对大肠杆菌、枯草杆菌及白色假丝酵母中的一种或多种拮抗指示菌具有抑制作用,占供测菌株总数的54.5%.在这些活性菌株中,红树源菌株拮抗比例为30.2%,非红树源菌株拮抗比例为69.8%.具有抗菌活性的菌株主要分布于15个属中,包括木霉Trichoderma、青霉Penicillium、拟青霉Paecilomyces和一些不产孢的分类群.某些稀有真菌如盘多毛孢Pestalotia、茎点霉Phoma也具有一定的抗菌活性.     

棕囊藻的生物学概述Ⅱ．生理生化学

棕囊藻Phaeocystis sp.独特的生理、生态学特征使它能在不同营养条件、光照强度和温度变化的环境中生长,并且由于受自身的毒性和粘液质的保护使它不能作为摄食者的食物而被大规模摄取,这使它不仅成为高纬度和极地海域一种重要的浮游植物类群,而且在中、低纬度海域它也能旺发,达到非常高的细胞密度,或形成赤潮.棕囊藻对不同生存环境的可塑性,归属于它独特的生理适应性和生理学的某些特点.棕囊藻特有的生物学特征使它在全球范围内高丰度、富分布,对改变整个生态环境存在潜在的威胁,应当引起有关单位的重视.     

Assessment of Lichen Vitality During a Transplantation Experiment to a Polluted Site

The present work evaluates the stress effects in two epiphytic lichen species with different thallus morphology, the foliose Parmelia caperata and the fruticose Evernia prunastri, as resulting from transplanting from an unpolluted to an air-polluted area. Lichen samples were collected in Portugal in a clean area, during the spring 2003, and transplanted (1) to the same zone nearby and (2) to a polluted area as affected by an industrial complex. Transplant samples were taken periodically during four months in both places. At the same time lichen samples from the clean-air site native (in-situ) populations were also collected. For each sample were measured the chlorophyll content, the chlorophyll degradation and the cell membrane damage, the latter represented by leacheate conductivity. During the experiment the meteorological conditions were registered. The results indicate the absence of stress effects of transplanting as such, and suggest that leachate conductivity may be the more sensitive indicator of general lichen vitality.     

使用多普勒超声成像比较栉孔扇贝与虾夷扇贝循环生理特征

将先进的多普勒超声成像技术运用于栉孔扇贝(Chlamys farreri)和虾夷扇贝(Patinopecten yessoensis)循环生理学的研究中,首次量化了扇贝心率(HR)、血流加速度(ABF)、收缩峰值速度(PS)、舒张末期速度(ED)、血液最大瞬时血流量(Max-IBF)、血液最小瞬时流量(Min-IBF)、收缩期与舒张期流速比(S/D)和阻力指数(RI)等指标,对比了两种不同耐温性扇贝的循环生理指标差异。结果表明,两种扇贝HR、ABF、PS、ED、Max-IBF、Min-IBF与水温均呈正相关;在10—25°C间,栉孔扇贝和虾夷扇贝HR范围分别为16—39、14—31次/min,ABF范围分别为1.75—15.84、0.99—7.41cm/s2,PS范围分别为3.54—8.09、2.32—8.85cm/s,ED范围分别为1.51—3.15、1.09—4.23cm/s,Max—IBF范围分别为0.051—0.193、0.137—0.316m L/s、Min—IBF范围分别为0.025—0.072、0.022—0.131m L/s、RI范围分别为0.49—0.69、0.42—0.54,S/D范围为2.28—2.83、1.75—2.12。同一温度下栉孔扇贝的HR、ABF、PS、ED指标水平均高于虾夷扇贝,Max-IBF、Min-IBF水平均低于虾夷扇贝;两种扇贝RI和S/D指标均在一定范围内波动。研究显示,虾夷扇贝在较低的心脏和血流搏动节律下保持了高效的血液流通,从而以相对较少的能量消耗维持了其机体代谢对血液循环的需求,这也与其冷水性的生态习性相适应。     

南极菲尔德斯半岛六种藻类和地衣植物的X荧光分析

为了研究南极的植物与环境间的关系 ,探讨植物在南极环境监测中的作用 ,在无标样条件下 ,利用微束 X荧光分析法对南极菲尔德斯半岛的 6种藻类和地衣植物进行了元素分析。其中藻类植物有孔石莼 (Ulva pertusa)、海膜 (H alymenia sp.)和大型鞘丝藻 (Lyngbya major) ,地衣植物有筛石蕊 (Cladonia borealis)、喇叭石蕊 (C.fimbriata)和松萝 (Usnea sp.)。研究结果发现 ,不同的藻类植物对各种元素的吸收能力不同 ,在 XRF(X射线荧光 )谱中 ,孔石莼比海膜含有更多的元素 ,象 As、Br和 Rb,Cu和 Zn的含量也较高。在大型鞘丝藻中 ,K比 Ca少 ,Zn的含量较高。在 3种地衣植物中 ,其 XRF谱中的元素种类组成非常相似 ,只是 Mn、Fe、Cu和 Zn等元素的相对含量有些差异     

Reform of Carbonate Rock Subsurface by Crustose Lichens and Its Environmental Significance

Crustose lichens are distributed extensively in karst areas in Southern China. They can be found on the surface of carbonate rocks. Through biophysical and biochemical processes, crustose lichens reform the subsurface of carbonate rocks and in the meanwhile change their physical and chemical properties: (1) the mechanical strength decreases by 17.04° on average (up to 33.2° ); (2) the chemical solution surface area increases from 28.26% to 75.36% (lichen microholes considered only); and (3) the water-holding capacity is greatly improved. Comparative field experiments between biokarst samples underneath crustose lichens and fresh rock samples with the same composition and texture show that the corrosional rate of carbonate rocks of the former is 1.264-1.643 times higher than that of the latter. Crustose lichens are considered as an activator of the surface corrosion of carbonate rocks.     

Factors influencing the distribution of coastal lichens Hydropunctaria maura and Wahlenbergiella mucosa

Responses to abiotic and biotic stresses that potentially drive the vertical zonation of the intertidal lichens Hydropunctaria maura, an upper littoral lichen, and Wahlenbergiella mucosa, a lower littoral lichen, were investigated in field and laboratory experiments. When transplanted, both lichens exhibited an inability to survive outside their normal vertical distribution range. W. mucosa appeared to be unable to tolerate prolonged periods of desiccation following translocation from lower to upper littoral regions, whereas H. maura was unable to survive in lower littoral zones possibly owing to increased grazing pressure. The effect of desiccation in both lichens was compared using pulse amplitude modulated chlorophyll fluorescence and infra‐red gas analysis; results indicated a more hydration‐dependent nature of W. mucosa. Photosynthetic (algal) pigments and phenolic compounds were determined in both lichen thalli, and a range of additional coastal lichens occupying a natural gradient from upper to lower shore levels. Pigment composition and concentration in both lichen thalli were similar whereas levels of phenolic compounds were up to three times higher in W. mucosa than H. maura. Pigment and phenolic concentration and composition exhibited some seasonality across 13 different lichens originating from different shore levels. Phenolic concentration increased towards the lower shore, suggesting a potential anti‐herbivory function. This marks the first study of pigments and phenolics in coastal lichen communities, and prompts further investigations on the particular physiological features of marine and maritime lichens that enable them to thrive in this extreme environment.     

移植操作胁迫对天鹅湖大叶藻存活、生长及光合色素含量的影响

2009年4月利用植株枚订移植法在山东荣成天鹅湖海域开展大叶藻植株移植,于5~10月监测移植大叶藻植株的存活率,并逐月采集自然和移植大叶藻植株,比较了植株生长、叶绿素含量和类胡萝卜素含量的变化,分析了移植操作胁迫对大叶藻存活、生长、叶绿素含量和类胡萝卜素含量的影响。结果显示,移植后1~2个月,移植植株的平均成活率较高,至移植后4个月,平均成活率降至57.8%,之后保持稳定。移植后6个月内,叶长移植植株均极显著低于自然植株;根长和茎节长在移植后1个月时移植植株显著低于自然植株,移植2个月后与自然植株无明显差异,甚至显著高于自然植株;叶绿素含量和类胡萝卜素含量在移植后1个月时移植植株显著低于自然植株,移植2个月后则与天然植株无明显不同,甚至显著高于自然植株,但10月份移植植株的叶绿素含量明显下降。结果表明,移植操作对大叶藻植株的地上部分产生了严重的胁迫作用和较长的胁迫时间,而移植植株的地下部分及叶片叶绿素和类胡萝卜素在经过短期胁迫后,能够通过自身的补偿机制分别实现快速生长和显著增加,从而利于植株的扎根、固着和提高植株的光合作用能力。     

4种典型滨海植物的耐盐能力

滨海植物是海岸生态系统的重要组成部分,而绝大部分滨海植物的耐盐能力与生态适应机制尚不清楚,本研究选择马鞍藤(Ipomoea pescaprae)、小刀豆(Canavalia cathartica)、全缘冬青(Ilex in?tegra)、大叶银边海桐(Pittosporum pentandrum)4种滨海典型植物,通过...     

胡麻生理生化代谢指标对干旱胁迫的响应及其模拟模型的研究

为进一步研究胡麻生理生化代谢指标响应干旱胁迫时对胡麻产量的影响,采用盆栽控水法模拟胡麻干旱胁迫,依据2013—2014年甘肃省定西市西巩驿镇胡麻试验数据,建立并检验胡麻干旱胁迫模型,模拟干旱胁迫时对胡麻生理生化指标及产量影响,利用均方根误差（RMSE）和决定系数（R²）描述模型拟合度。结果表明：（1） 模型产量模拟值的RMSE为41.3159~155.6685 kg·hm^-2,平均值为80.1837 kg·hm^-2;R²为0.8929~0.9894,平均值为0.9387,该模型具有较好的拟合度、可行性也较强。（2） 在重度干旱胁迫下,抗氧化代谢指标——超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）中,CAT活性表现趋势为下降,终花期POD活性增幅（26.09%~28.00%）最大;渗透调节的3种物质含量均显著上升,其中脯氨酸增幅最大,达236.22%。（3） 呈现出极显著相关性的各指标有3组,分别是脯氨酸与叶绿素、丙二醛和CAT活性;可溶性蛋白与丙二醛和CAT活性;POD活性与SOD活性。胡麻生理生化指标响应不同程度干旱胁迫与胡麻的产量有极强的关联,此模型的建立是对胡麻生理生化指标响应干旱胁迫的科学补充,进一步为胡麻的高效生产管理及农业生产系统提供理论依据和支持。