Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).     

Response of antioxidant systems to copper in the gills of the clam Ruditapes decussatus

Copper (Cu) is an essential element for biological systems, however, when present in excess, is toxic. Metallothioneins can play an important role in Cu homeostasis and detoxification. Moreover, Cu can catalyse the production of toxic hydroxyl radicals that cause lipid peroxidation but defence systems in the cells can limit the oxidative damage. The present study was performed to investigate the effect of three Cu concentrations (0.5, 2.5 and 25 μg l⁻¹) on the response of antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), selenium-dependent glutathion peroxidase and total glutathion peroxidase), total proteins, metallothioneins (MT), malondialdehyde (MDA) concentrations in the gills of the clam, Ruditapes decussatus. The activity of antioxidant enzymes and total proteins, MT and MDA concentrations were measured in the gills of the clams after 1, 3, 7, 14, 21 and 28 days of Cu exposure. Results indicate that Cu only induces an imbalance in the oxygen metabolism during the first week of Cu exposure due to a decrease in mitochondrial SOD and CAT, selenium-dependent and total glutathion peroxidase activities. Cu also causes lipid peroxidation, measured by the MDA formation, that was Cu dependent. In the gills of clams exposed to 25 μg Cu/l, the excess of Cu triggers the induction of MT synthesis after 3 days of exposure.     

温度变化对刺参同工酶的影响

采用同工酶电泳方法,对在不同温度处理下刺参(Apostichopus japonicus)的乳酸脱氢酶(LDH)、酯酶(EST)和超氧化歧化酶(SOD)的表达情况进行分析。本文结果显示刺参组织内无LDH同工酶的表达。在10℃到20℃的温度突变处理中,12 h内EST和SOD均有新增酶带,在20℃到10℃的温度突变1 h内EST表达有新增酶带。3 h内SOD表达有新增酶带。在不同变温幅度的处理中,EST和SOD只有1条酶带。这表明,刺参对温度突变可产生积极的响应,对于其适应环境温度的变化具有重要意义。     

新疆温泉县北达巴特斑岩铜钼矿地质特征及成因初探

北达巴特斑岩铜钼矿产于华力西期流纹斑岩中,矿体呈脉状,铜矿体地表为氧化物,钼矿体主要赋存于深部的流纹斑岩中,其矿化作用呈上铜下钼的双层矿化结构模式,矿化为细脉浸染状。矿床的成因类型为斑岩型铜钼矿。     

利国矿区铜（金）成矿条件的研究

利国矿区是江苏省重要铁矿基地之一,矿区位于秦岭一昆仑纬向构造带东延的南侧与新华夏构造体系第三隆起带的交接复合部位。铁矿床主要产于燕山期闪长玢岩杂岩体与下奥陶统碳酸盐地层的接触带及其附近灰岩中,矿体呈似层状一透镜状等,产状与围岩一致,具层控矿床的特征。笔者主要从成矿地质特征着手,分析总结了该区铜、金矿化特征及主要成矿规律,指出了利国矿区铜（金）矿的找矿方向和有望靶区。     

二三维联动的虚拟校园设计与实现

传统的2维地理信息系统技术成熟但缺乏3维可视化和分析的能力,而目前的3维地理信息系统发展可拥有3维可视化分析能力,然而还缺少2维地理信息系统的便捷。以二三维联动为开发思路的地理信息系统能够解决这对矛盾。本文基于二三维联动GIS的设计方案,以虚拟校园设计与实现为应用实例,利用ArcEngine（AE）实现二三维联动GIS系统的开发设计,对GIS系统的开发做出了一种新的、有益的尝试。     

三峡库区高切坡致灾因素及防护对策——以重庆市万州区为例

三峡库区百万移民的搬迁建设导致大量高切坡的形成。未及时治理或治理不当的高切坡,不同程度地发生破坏,影响了人们的正常生活,甚至引发了地质灾害。因此,科学认识及治理高切坡十分必要。重庆市万州区高切坡主要有风化及差异风化、崩塌和土体滑塌等破坏形式。影响破坏的因素主要为工程地质条件、气候及时间效应。针对高切坡不同的破坏形式,以安全、经济及环境和谐的人文理念作为设计指导思想,充分论证了高切坡的防护方案,即：削坡、排水以及格构防护较适合万州区高切坡的防护。这些措施,既能很好地解决高切坡的防护问题,又能兼顾环保、降低造价。研究结果可以作为三峡地区高切坡类地质灾害防护设计的参考。也可为其它流域类似问题借鉴。     

含盐量对松嫩平原碳酸盐渍土抗剪强度的影响

松嫩平原分布着大量的碳酸盐渍土,其不良的工程性质给穿越其中的道路质量造成不同程度的影响。由于盐渍土所含易溶盐成分主要为碳酸氢钠,该研究通过向碳酸盐渍土内加入碳酸氢钠制备不同含盐量的盐渍土,进行室内直剪试验。试验表明：随着含盐量的增加土的粘聚力减小,内摩擦角有先减小后变大的趋势;随着含水量的增加,土的含盐量越高,土的粘聚力减小越明显。易溶盐在土中的状态形式是影响其抗剪强度参数变化的一个重要原因。     

GM（1，1）动态模型在吴江市地下水水位预测中的应用

以吴江市地下水水位预测为例,详细阐述了地下水水位时间序列的GM（1,1）动态模型的原理和建立过程,并根据模型的预测值和实测值,对模型的精度进行了检验,结果表明,模型的预测精度达到了99．27％,等级属于Ⅰ级,具有实际的应用价值,为地下水资源的科学管理提供了依据。