东海原甲藻cDNA文库构建及尝试性EST分析

东海原甲藻(Prorocentrumdonghaiense)是中国沿海频繁发生的大规模赤潮的原因种之一,大规模分离鉴定东海原甲藻功能基因是理解东海原甲藻赤潮形成过程和机理的重要基础。取对数生长期藻体,经微量总RNA抽提、cDNA合成、cDNA扩增和克隆等步骤,构建了东海原甲藻cDNA文库并进行了尝试性表达序列标签分析。从含有约5000个转化子的文库中随机取150个测序,获得126条EST序列。经网上BlastN及BlastX分析,共发现11个是已知功能的基因的标签。这些基因与东海原甲藻生长发育、物质转换和能量代谢相关。     

RFID技术在海洋档案中的应用

射频识别(RFID)技术是一种非接触式的自动识别技术,它作为一种新型的自动识别技术将极大地推动信息社会的发展.本文简单介绍了RFID的技术特性,探讨了RFID技术在海洋档案领域的应用,并阐述其在海洋档案大规模使用过程中存在的问题和对RFID技术在海洋档案领域发展趋势的展望.     

空间信息服务在RFID物流管理系统中的应用研究

RFID（射频识别）中间件技术是RFID产业化的关键技术,分布式环境下的信息交换是这项技术的主要研究方向之一。随着企业对空间位置信息的关注需求日益增大,空间信息服务也成为RFID中间件中必不可少的组成部分。本文介绍了RFID中间件和空间信息服务的概念,通过一个RFID物流管理系统应用实例研究了空间信息服务在RFID中间件中提供空间信息操作,处理与交换的应用。     

Evidence of marine mammal predation of the European eel (Anguilla anguilla L.) on its marine migration

Temperature and depth logging tags were implanted into adult eels released on Atlantic west coasts of France and Ireland to study their oceanic migration behavior. For three of the tags, 25 to 256 days after release there was a dramatic rise in temperature from 10 °C to 36 °C and the dive profile changed from depths of 300–1000 m to repeated ascents to the surface. This indicated that the eels carrying the tags had been eaten by a mammalian predator. Two of the tags had sufficient sampling rate to resolve the dives in detail. They recorded a total of 91 dives to maximum depths of 250–860 m lasting 11–12 min and with surface intervals of 5–7 min. More than two thirds of the dives included a rapid descent from approximately 500 m to 600–700 m. From this we infer that the predator was most likely a deep-diving toothed whale. The dives logged while the tags were inside the predator revealed that the temperature usually decreased during dives, and increased again during surface periods. The temperature drops during dives were probably caused by the ingestion of prey or water. These observations provide insights into the behavior of toothed whales foraging in the mesopelagic zone.     

基于物联网GIS的消防智能巡检系统设计与实现

消防设施的高效智能管理是衡量城市智慧化水平的重要指标。为了实现该目标,利用物联网技术以及网络地理信息服务技术,设计并实现了集移动巡检终端与网络后台信息管理系统于一体的消防设施智能巡检系统。该系统利用智能无线传感器、RFID以及蓝牙无线通讯等技术,实现消防设施非接触式自动巡检与巡检信息实时传输;通过基于GIS的消防管线与消防拴拓扑结构分层分级可视化,实现消防设施的统一高效管理。相比于现有巡检方式,基于物联网GIS的消防设施智能巡检系统提高了巡检效率与便捷性以及巡检信息的准确性与实时性,为城市中消防设施的实时动态智慧化管理提供了技术支撑和保障。     

农产品物流监控系统设计与开发研究

对农产品物流的监控可以及时发现农产品在流通环节的问题。结合无线传感网(WSN)技术、GIS技术、GPS技术、RFID技术,集成成熟的无线通信和数据库技术,构建可视化、智能化、通用的农产品物流监控系统,为物流链管理者提供便捷、全面的监控体系。本文介绍了系统的设计与实现。     

哈雷彗星主尾流的研究

哈雷彗星在日彗距较大时出现长而直的主彗尾（尾流），这是很有趣的。尾流一般是指等离子体尾流；但是，当地球接近彗星轨道面时，尘埃尾流可能叠加到主彗尾上。在一般感光波段宽的彗星底片上很难区分这两种尾流。本文选取哈雷彗星在不同日彗距的5条主尾流，作了光度测量和比较分析。得出沿各尾轴及其垂直方向几个截面的亮度分布、亮度半极大全宽、尾轴的视风差角和真风差角及彗尾长度。在所分析的蓝敏底片上，过近日点前的2个尾流肯定是等离子体尾流，而5个尾流的相似性以及其他证据说明它们主要都是等离子体尾流，尘埃彗尾的污染是次要的。     

物联网在人工影响天气装备弹药管理中的应用

本研究以物联网技术为基础,利用条码、二维码和射频电子标签标识、射频扫码技术、声光电自动感应技术、GPS/GIS技术进行信息采集,建立人工影响天气装备弹药物联网管理系统,实现人工影响天气装备弹药从生产、验收、转运、仓储到发射作业的全程监控。在北京、陕西、贵州、河南4个地区进行试点开发研究,根据有源/无源射频识别(RFID)、二维码/条形码、火箭弹/高炮炮弹以及信息采集技术分别开展不同技术模式的应用试验,将弹药信息按照统一格式汇集至国家级物联网系统,有效提高了全国人工影响天气装备弹药信息采集的准确性和时效性,并结合有效传感器、无线通信技术,解决了大范围内的作业数据自动化采集及地面作业信息实时监控,提高人工影响天气作业安全管理的科技水平和业务信息化现代化程度,对全国开展人工影响天气装备物联网建设工作具有较强的参考价值。     

Assessment of a new solution for tracking pebbles in rivers based on active RFID

Low‐frequency passive integrated transponders (PIT tags), are commonly used for monitoring pebble mobility in gravel‐bed rivers. Although early studies reported high recovery rates for PIT tags used in small streams, recovery rates in larger systems remain low, substantially limiting the possibilities for their use in such rivers. These low recovery rates are potentially due to missed detections caused by tag signal collision, burial in the sediment layer deeper than the maximum detection range and insufficient (but still exhausting) field effort to cover the concerned areas. A potential solution for addressing these problems is to use active ultra‐high frequency (a‐UHF) transponders as these have a greater detection range and anti‐collision protocols. In order to assess the potential of such transponders for pebble tracking in rivers, we used 433.92 MHz COIN‐ID and COIN‐HC models (ELA Innovation Company, Montpellier, France). We completed several tests to (i) characterize transponder detection ranges in the water and in saturated sediment and (ii) develop field protocols for locating tags by combining global positioning systems (GPS) sites and transponder received signal strength indication (RSSI) levels. The results showed that (i) the maximum detection ranges are about 2.4 m in the water column and more than 2.6 m in a column of saturated gravelly‐sandy sediment, (ii) RSSI spatial interpolation can be used to determine transponder position with good accuracy (< 1 m), (iii) the desired minimal level of accuracy can be adjusted depending on in‐field effort and signal impulse interval, (iv) the RSSI maximal value observed cannot yet be used to determine transponder burial depth because of the multipath propagation of radio frequencies and the semi‐directional emission of the tag signal. Copyright © 2017 John Wiley & Sons, Ltd.