Assessment of Lichen Vitality During a Transplantation Experiment to a Polluted Site

The present work evaluates the stress effects in two epiphytic lichen species with different thallus morphology, the foliose Parmelia caperata and the fruticose Evernia prunastri, as resulting from transplanting from an unpolluted to an air-polluted area. Lichen samples were collected in Portugal in a clean area, during the spring 2003, and transplanted (1) to the same zone nearby and (2) to a polluted area as affected by an industrial complex. Transplant samples were taken periodically during four months in both places. At the same time lichen samples from the clean-air site native (in-situ) populations were also collected. For each sample were measured the chlorophyll content, the chlorophyll degradation and the cell membrane damage, the latter represented by leacheate conductivity. During the experiment the meteorological conditions were registered. The results indicate the absence of stress effects of transplanting as such, and suggest that leachate conductivity may be the more sensitive indicator of general lichen vitality.     

Hydrogeology and hydrochemistry of the Parmelia aquifer,northern Perth Basin,Western Australia

This study aims to characterise the hydrogeology and hydrochemistry of the Parmelia aquifer and to understand controls on recent water-level changes as these are needed to underpin a quantitative analysis of recharge. The Parmelia aquifer, a layered sequence of sand, silt and discontinuous lenses of clay, receives diffuse rainfall recharge on its outcrop and groundwater recharge occurs across the Dandaragan Plateau at different rates. Water levels have risen steadily over the last three decades between 10 and 55 cm/y in response to the replacement of native vegetation with pasture and annual crops. The mean aquifer properties from sediment analyses indicate a very wide range of porosity (8.9 – 49.5 %) with an arithmetic mean of 26% and consequently a very broad range of specific yield (0.0004 – 0.4) with an arithmetic mean of 0.14. Groundwater in the Parmelia aquifer has an underlying meteoric origin with compositional changes due to reactions with silicate minerals and leaching of chloride that has concentrated in the soil by evapotranspiration. The hydrochemistry sampled at different depths and locations in the aquifer indicates that the groundwater is not well mixed, and variations arise due to relatively recent recharge that has undergone evaporation in some areas.     

Bioaccumulation of Cobalt in Parmelia sulcata

Six locations across mainland Portugal were selected for exposing Parmelia sulcata, for a one-year period (8 months for one site), with simultaneous measurement of total (dry + wet) deposition (one-month periods). The exposed lichens and the total (dry + wet) deposition were analysed for cobalt contents by INAA (instrumental neutron activation analysis) and ICP-MS (inductively coupled plasma mass spectroscopy), respectively. The designated wet deposition was evaluated through the collected water volume; the designated dry deposition was assessed after the (dried) residual mass of the wet deposition. An excellent agreement between Co contents in exposed lichens and the cumulative (1) Co contents in the dry deposition, (2) dry deposition, and (3) wet deposition has been found for the locations with alternate drought and precipitation months, high dry deposition, and high Co contents in the latter. Continuous rainfall was found to hinder the Co accumulation in the lichen due to its release from the lichen and/or lower Co contents in the dry deposition. At three locations, P. sulcata Co contents, after subtraction of the background (before exposure), equalled or exceeded the Co contents in the cumulative dry deposition at the end of the exposure time. The optimal exposure period for this species likely depends on the exposure conditions.     

Biomonitoring of Trace Metals by Different species of Lichens (Parmelia) in North-West Italy

The concentrations of Cd, Cu, Mn, Ni, Pb, V, and Zn were measured in four different species of lichens (Parmelia caperata, P. pastillifera, P. saxatilis, P. sulcata) sampled in North-West Italy, in order to obtain maps showing metal distribution and the degree of deviation from background (natural) conditions. Significant differences among the species were found for Zn, Mn, Pb and V, suggesting that the combined use of mixed species in biomonitoring surveys can affect the interpretation of results. Results obtained for Parmelia caperata samples have been compared with background (natural) levels, and maps showing the distribution of each metal expressed as deviation from background levels have been obtained. Overall such maps show a medium to high alteration degree in the study area.