Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

罗氏沼虾育苗用水中Mg2+与Ca2+含量及Mg2+/Ca2+对出苗率的影响

１９９１年３－６月和１９９２年３－６月在南汇县东海水产养殖公司对罗氏沼虾育苗用水调配原则与不同基础水的调配方法进行试验研究。结果表明，育苗用水中Ｍｇ＾２＋与Ｃａ＾２＋含量及Ｍｇ＾２＋／Ｃａ＾２＋必须达到一定的范围，这是调配的基本原则。以鱼塘水作基础水时，需添加６种化学药品，其中Ｍｇ＾２＋与Ｃａ＾２＋含量可适范围分别为３００．０－４４０．０ｍｇ／Ｌ与１７０．０－２４４．０ｍｇ／Ｌ，Ｍｇ＾２＋／Ｃａ＾     

白沙河地下水资源管理模型

针对白沙河地下水含水层的内部结构和本区开采特点以及已出现的环境问题，建立了地下水资源管理模型，确定了优化开采方案。该管理模型针对本区每年２个管理时段的长度及其开采量均不同的特点，导出了新的响应矩阵的公式。管理模型的建立有助于对该区地下水的合理开采。     

Possible Density Change of the Origin of North Pacific Intermediate Water Due to Double Diffusion in the Mixed Water Region

In this study we test Talley's hypothesis that Oyashio winter mixed-layer water (26.5–26.6σ _θ) increases its density to produce the North Pacific Intermediate Water (NPIW) salinity minimum (26.7– 26.8σ_θ) in the Mixed Water Region, assuming a combination of cabbeling and double diffusion. The possible density change of Oyashio winter mixed-layer water is discussed using an instantaneous ratio of the change of temperature and salinity along any particular intrusion (R _l ). We estimate the range of R _l ^DD required to convert Oyashio winter mixed-layer water to the NPIW salinity minimum due to double diffusion, and then assume double-diffusive intrusions as this conversion mechanism. A double-diffusive intrusion model is used to estimate R _l ^DD in a situation where salt fingering dominates vertical mixing, as well as to determine whether Oyashio winter mixed-layer water can become the NPIW salinity minimum. Possible density changes are estimated from the model R _l ^DD by assuming the amount of density change due to cabbeling. From these results, we conclude that Oyashio winter mixed-layer water contributes to a freshening of the lighter layer of the NPIW salinity minimum (around 26.70σ_θ) in the MWR.     

济州岛南部海区的双跃层现象

本文分析了济州岛南部区域温度双跃层现象的类型及其成因。特别阐明了双跃层的波状现象,指出:强流锋区的侧向效应,黑潮次—中层混合水的爬升,黄海冷水团边缘密度环流引起的侧向流动,以及上、下层流速、流向不一致引起的剪切作用,是导致双跃层波状现象的主要原因。发现上、下跃层之间存在着“跃层间环流”,这是一个有趣的海洋学现象,它将导致双扩散的发生和有利于双跃层的维持。     

中华鲟配合饲料适宜蛋白质含量及最佳蛋白能量比研究

以酪蛋白和明胶为蛋白源 ,添加豆油、鱼油、糊精、纤维素以及适量的维生素和矿物质混合剂配制试验饲料 ,饲料配成5个蛋白质水平 ,每个蛋白水平分别包括5个能量级 ,以体质量增长倍数、蛋白质效率和饲料系数为评判依据 ,对体质量为8.73g±0.79g的中华鲟(Acipensersinensis)的适宜蛋白质含量及其蛋白能量比进行了研究。经过为期8周的试验 ,结果表明 :中华鲟幼鲟配合饲料中的适宜蛋白质含量范围为35.7%～41.8% ,饲料的最佳蛋白能量比范围为21.85～30.05ng/J。当试验饲料蛋白质含量为40.3% ,蛋白能量比为27.85ng/J时 ,试验鲟鱼获得最大体质量增长倍数为11.60±1.20,最低的饲料系数为1.68±0.23,以及最高的蛋白质效率为1.58%±0.14%。     

Structure of Subsurface Intrusion of the Oyashio Water into the Kuroshio Extension and Formation Process of the North Pacific Intermediate Water

In order to understand the actual formation process of the North Pacific Intermediate Water (NPIW), structure of subsurface intrusions of the Oyashio water and the mixing of the Oyashio and the Kuroshio waters in and around the Kuroshio Extension (KE) were examined on the basis of a synoptic CTD observation carried out in May-June 1992. The fresh Oyashio water in the south of Hokkaido was transported into KE region through the Mixed Water Region (MWR) in the form of subsurface intrusions along two main paths. The one was along the east coast of northern Japan through the First Branch of the Oyashio (FBO) and the other along the eastern face of a warm streamer which connected KE with a warm core ring through the Second Branch of the Oyashio (SBO). The fresh Oyashio water extended southward through FBO strongly mixed with the saline NPIW transported by the Kuroshio in the south of Japan (old NPIW) in and around the warm streamer. On the other hand, the one through SBO well preserved its original properties and extended eastward beyond 150°E along KE with a form of rather narrow band. The intrusion ejected Oyashio water lens with a diameter of 50–60 km southward across KE axis and split northward into the MWR involved in the interaction of KE and a warm core ring, which were supposed to be primary processes of new NPIW formation.     

黄、东海毗邻海域悬浮体与水团的对应关系及影响因素

对黄、东海毗邻海域由陆架到深海的悬浮体进行了大量加密取样,获得了较准确的结果。给出了悬浮体含量及其三维分布,确定它们与水团及其边界有良好的对应关系。并从物源、动力和成因等解释了这种对应,提出了水团对悬浮体研究的重要意义。研究表明黑潮次—中层混合爬升水及其混合水形成悬浮体含量最低值区,横亘在陆架与深海之间,形成阻隔含有大部分悬浮体的陆架中、下层水体向深海输送的洁净“水障”,台湾暖流北上的顶托也起类似作用,并分析了影响悬浮体含量的各种因素及有关机制。     

多尺度空间线状实体形状相似关系的表达与度量

本文对空间数据多尺度表达中线状实体综合前后的形状相似性进行了描述、分析和研究。通过调查统计,得出了衡量线状实体形状相似程度的两个重要参量SIMⅠ、SIMⅡ,基于这两个参量给出了空间线状实体形状相似性的定义以及在不同尺度下线状实体形状相似性的度量方法,并用实例进行验证。为空间数据多尺度表达时线状实体的形状表达提供了一种分析和评价手段。     

Voronoi图的首最邻近递归收敛特性及其应用

讨论了Voronoi图的首最邻近递归收敛特性,即从任一伪最邻近对象开始,递归查找其首最邻近对象,最后必然收敛于最邻近对象.利用该特性,初步实现了移动目标直接邻近对象的连续查询.