WOFOST模型对河南省冬小麦模拟的适用性分析

为进一步研究WOFOST模型在河南省冬麦区的适用性,以河南省30个农业气象观测站1991—2014年冬小麦观测资料、历史气象资料和土壤资料为依据,对WOFOST模型进行逐站调参和验证,分别建立了30个站的冬小麦模型参数。其中1991—2010年为模型调参年份,2011—2014年为模型验证年份。各站开花期和成熟期调参模拟的归一化均方根误差NRMSE分别小于5%和3%,验证误差分别为3.7%和2.9%。除潢川和固始外,模型对其余各站产量模拟的归一化均方根误差NRMSE全省各站均小于20.0%,验证误差全省平均为15.2%,大部分站点观测值和模拟值相关系数r通过了显著检验。利用调参后的模型模拟2011—2014年冬小麦生长动态变化可知,模拟地上部总干物重与实测单株干物重、模拟LAI与单株叶面积有较一致的变化趋势,拟合度较高。因此,WOFOST模型对河南省冬小麦主要发育阶段、产量及干物质积累模拟能力较强,具有良好的应用前景。     

考虑双向水平地震作用的结构设计问题研究

分析了抗震设计中考虑双向水平地震作用的必要性,指出了研究双向地震作用的必要性,通过国内外规范相关条文的对比指出目前研究有待完善的关键问题和方向,结合近期研究成果对其中几个值得注意的问题提出建议。     

带深桩基础高层建筑结构的地震动输入问题

带深桩基础高层建筑结构的地震反应分析通常按刚性地基假定选择地震动输入,其合理性一直受到工程界关注。本文探讨了此类结构考虑桩-土-结构相互作用的整体有限元分析的地震激励施加、单元网格划分、积分步长确定、边界处理等问题,通过整体有限元分析模型和常规分析模型的算例分析对比,探讨了常规地震动输入方法的合理程度。结果表明,常规输入法总体偏于安全,是基本可行的,但可能低估结构底部几层的地震反应,应引起注意。     

四川省拐卖儿童犯罪的时空演变过程及影响因素分析

拐卖儿童犯罪给受害者带来身心创伤、给家庭带来巨大悲痛和长期困扰,已成为社会和学界关注的焦点问题。该领域犯罪地理学视角的研究兴起较晚,且主要侧重宏观整体分析,当前亟需聚焦高发源地和重点人群作精细解析。为此,论文针对中国拐卖犯罪重灾源区四川省,综合运用数理统计、空间分析、文本分析、案例分析等方法探究四川省拐卖儿童犯罪的时空演变过程与影响因素。研究发现：① 数量上,被拐男童明显多于女童,被拐儿童主要来自乡村,虽呈现1~6岁和14~17岁的“双峰”型特征,但整体趋向低龄化。② 时间上,犯罪年际分布大致呈倒“V”型波动态势,高发期为1981—2000年;犯罪年内分布集中于夏半年,周内分布周末高发,日期分布每月1日的被拐儿童数量最多。③ 空间上,存在一个被拐高发区和多个被拐次高发区;县域尺度上的犯罪格局呈现“高—高”和“低—低”聚集;随着时间的变化,拐出热点区不断向南部和东部蔓延;宏观贩运路径上,向中东部地区分散拐入,华北和华南是重点拐入的“南北两汇”。④ 对1981—2000年犯罪高发期的原因解析发现,受教育水平与城乡收入差距是影响拐卖儿童犯罪的主导因素。     

2019年6月17日长宁MS6.0地震前后地壳振动异常分析

宽频带地震仪可以连续不断地记录地面振动信号,进而探索地壳振动的变化与地震前兆之间的物理关联性.选取2019年6月17日四川长宁Ms6.0地震前后的震中500km范围内15个宽频带地震仪的连续波形数据,通过傅里叶变换计算分析地震前后的地壳振动低频信号(8×10-5～2×10-4Hz)的振幅谱变化,并对低频信号源进行定位,...     

Growth and feed efficiency of juvenile shrimp Litopenaeus vannamei fed formulated diets containing different levels of poultry by-product meal

This feeding trial was conducted to evaluate the potential of poultry by-product meal (PBM) as a protein source in the culture ofLitopenaeus vannamei. Seven isonitrogenous and isoenergetic diets were formulated to near to commercial diet with about 40% protein and 7.5% lipid. Fish meal was replaced by 0, 30%, 40%, 50%, 60%, 70% and 100% of PBM (diets 1-7). The diet with 100% fish meal was used as a control (diet 1). Post-larvae were reared in an indoor semi-closed re-circulating system. Each dietary treatment was tested in 4 replicate tanks (260 L) of 40 shrimp, arranged in a completely randomized design. The shrimps were hand-fed for three times a day to near-satiation (0700, 1200 and 1800) for 60d. Percentage weight gain, survival, feed conversion ratio (FCR), protein efficiency ratio (PER) and body composition of shrimps were measured. There were no significant differences (P>0.05) in growth performance among shrimps fed diets 1-5 (0-60% fish meal replacement). However, shrimps fed diet 7 (100% fish meal replacement) had significantly lower (P<0.05) growth than those fed diets 1-5 (0-60% fish meal replacement). Shrimp fed diets 2-4 (30%-50% fish meal replacement) showed significantly higher growth than those fed diets 6 and 7 (70% and 100% fish meal replacement, respectively). Survival ranged from 94.7% to 100.0% and did not differ significantly (P>0.05) among different experimental diets. No differences in body composition were found among shrimps fed different diets. These results showed that up to 70% of fish meal protein can be replaced by PBM without adversely affecting the growth, survival, FCR, PER and body composition of Litopenaeus vannamei.     

基于客观识别的冬季南支槽强度年际变化及其与南方降水的关联性

利用欧洲中心ERA Interim逐日再分析资料,基于南支槽客观识别方法定义了一个南支槽强度指数,并结合格点降水资料,对1980—2019年冬季南支槽强度的年际变化特征及其与中国降水、大气环流和水汽输送的联系进行了研究。结果表明：① 南支槽强度变化主要呈准4年周期。② 赤道中东太平洋海温是影响南支槽年际变化的一个重要因素,与南支槽强度有很强的负相关关系,其中Nino 3区的海温变化对南支槽强度的影响更显著。③ 南支槽偏强年从云南南部到华南一带降水量较常年偏多,其中广东、湖南和江西南部至福建一带降水量较常年偏多30 mm以上;南支槽偏弱年西南至华南一带降水较常年偏少,其中广东、广西东部降水量较常年偏少30 mm以上。④ 南支槽偏强年时存在两支异常水汽输送路径,一支为从赤道以北至10°N,从60°E延伸至菲律宾附近的异常强西风水汽输送带;另一支位于20°N附近,由南支槽的槽前西南气流将孟加拉湾地区水汽输送到我国境内,是冬季西南与华南的主要水汽通道。     

Rapid,specific and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification targeted to vvhA gene

Vibrio vulnificus is an estuarine bacterial pathogen for human.The rapid,specific and sensitive detection of V.vulnificus is urgently needed for early disease diagnosis and timely treatment of V.vulnificus infection.In the study,a loop-mediated isothermal amplification(LAMP) technique was developed for V.vulnificus detection with a set of primers,composed of two out primers and two inner primers targeted to vvh A gene.The optimal amplification temperature was 63°C and the reaction only took 35 min.The amplification products could not only be detected by agarose gel electrophoresis with ladder-like pattern bands,but also could be visualized using calcein with naked eye directly.Forty-five strains were tested for the specificity of LAMP assay,and all the V.vulnificus strains were identified correctly while other strains were negative results.The sensitive of the new LAMP assay was 100-fold more sensitive than the conventional PCR.Meanwhile,all the V.vulnificus strains were detected correctly in spiked,clinical and environmental samples by the new LAMP assay.Compared with other well-known techniques,the new LAMP assay targeted to vvh A gene was extremely rapid,simple,sensitive and specific for V.vulnificus identification.     

利用扩增vvhA基因的环介导等温扩增技术快速，特异而敏感地检测创伤弧菌

创伤弧菌是一种可感染人类的河口病原菌。建立快速,特异而敏感的检测方法,有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物（包括两对外部引物和两对内部引物）,采用环介导等温扩增技术（LAMP）来检测创伤弧菌。结果显示,本方法的最适扩增温度是63℃,反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出,也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性,其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍,同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较,针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。     

Comparative analysis on microbial community associated with different gastrointestinal regions of wild northern snakehead Channa argus Cantor, 1842

Microbial communities in different gastrointestinal regions(stomach, foregut, midgut, and hindgut) of the northern snakehead C hanna argus(Cantor, 1842) were compared by polymerase chain reaction and partial 16 S r DNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant dif ferences were found in the Sobs, ACE, Shannon, and Simpson indices among samples(P 0.05). The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either H alomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firmicutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant diff erences were found in the compositions of microbial community among the four regions( P 0.05). Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut( P 0.05). Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively(P 0.05). Proteobacteria was the lowest in the hindgut( P 0.05). At genus level, C etobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples( P 0.05). Clostridium and Prevotella were the highest in the midgut(P 0.05). Halomonas, Shewanella, and S phingomonas were the highest in the foregut( P 0.05). Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows: stomach, P aracoccus and Vibrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, C etobacterium and Plesiomonas( P 0.05). At the species level, A cinetobacter rhizosphaerae was only detected in the stomach. P revotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this study indicated that microbial community was not only related to rearing environment but also to the physico-chemical characteristics of each gastrointestinal region.